Fermentation method for producing N-acetylneuraminic acid

A technology of acetylneuraminic acid and fermentation method, applied in the field of fermentation for producing N-acetylneuraminic acid, can solve problems such as low yield, limited application scope, high cost, etc., and achieve the effects of increasing yield and improving synthesis rate

Active Publication Date: 2019-04-16
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI +1
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Although the cost of NeuAc derived from genetically engineered bacteria has been greatly reduced, the yield is still relatively low, and the high cost limits its application range

Method used

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  • Fermentation method for producing N-acetylneuraminic acid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The fermentation method for producing N-acetylneuraminic acid provided in this embodiment includes the following steps:

[0043] 1 Strain activation:

[0044] The frozen N-acetylneuraminic acid-producing strains (preservation number CCTCC No: M2013494) were streaked and inoculated onto solid LB medium and cultured at 37°C.

[0045] 2 Cultivation of primary seeds:

[0046] When a single bacteria grows out, pick a single colony, inoculate it into an Erlenmeyer flask containing 50 mL of liquid LB seed medium, and cultivate it overnight at 37°C at 200 rpm to obtain a first-level seed liquid.

[0047] 3 Cultivation of secondary seeds:

[0048] The first-level seed liquid was inoculated into an Erlenmeyer flask containing 50 mL of liquid seed culture medium at an inoculum of 1%, and incubated at 200 rpm and 37° C. for 6 hours to obtain a second-level seed liquid.

[0049] The liquid LB medium contains: peptone 10g / L, yeast extract powder 5g / L, sodium chloride 10g / L, pH 7.0.

[0050] The s...

Embodiment 2

[0066] The fermentation method for producing N-acetylneuraminic acid provided in this embodiment is basically the same as that of Embodiment 1, except that the feed solution in this embodiment contains 50% glycerol and 2.5% N-acetylglucosamine.

[0067] The results showed that the NeuAc yield was 82.3g / L and the average synthesis rate was 16.46g / L*12h after 60h of fermentation using the fermentation method of this example.

Embodiment 3

[0069] The fermentation method for producing N-acetylneuraminic acid provided in this example is basically the same as that in Example 1, except that the feed solution in this example contains 50% glycerol and 1% polyphosphoric acid.

[0070] The results showed that the NeuAc yield was 76.8 g / L and the average synthesis rate was 15.36 g / L*12h after 60 hours of fermentation using the fermentation method of this embodiment.

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Abstract

The invention discloses a fermentation method for producing N-acetylneuraminic acid and relates to the technical field of N-acetylneuraminic acid production. The fermentation method for producing N-acetylneuraminic acid comprises the steps of adding supplementary feed liquid to a fermentation culture medium inoculated with N-acetyl-neuraminic acid bacteria during fermentation, wherein the feed liquid contains glycerin and an additive, the additive is selected from one or more of N-acetylglucosamine and polyphosphoric acid substances. The fermentation method improves the synthesis rate of N-acetylneuraminic acid and meanwhile improves the yield of the N-acetylneuraminic acid by optimizing the composition or use amount of the feed liquid.

Description

Technical field [0001] The present invention relates to the technical field of N-acetylneuraminic acid production, in particular to a fermentation method for producing N-acetylneuraminic acid. Background technique [0002] N-acetyl neuraminic acid (N-acetyl neuraminic acid, NeuAc) is an acidic amino sugar with 9 carbon atoms and a pyranose structure. It is one of the main members of the sialic acid family. NeuAc is widely distributed in mammals and is an important part of cell membrane proteins. It participates in various physiological functions of the body: 1) anti-recognition effect; 2) improving infants’ intelligence and memory; 3) inhibiting leukocyte adhesion and anti-inflammatory; 4) anti-tumor , Anti-virus; 5) Anti-Alzheimer's, etc. NeuAc is widely used in the field of food, health products, cosmetics, pharmaceutical precursors and disease diagnosis, etc., and has significant market value. [0003] At present, the main production methods of N-acetylneuraminic acid include:...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/02C12N1/19
CPCC12P7/6436C12P13/02
Inventor 吴金勇陈祥松李翔宇姚建铭王纪袁丽霞王煜朱薇薇
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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