Fermenting preparation method of oosporein

A technology of oosporin and bacterial strain, which is applied in the field of oosporin fermentation and preparation, can solve the problems of unreachable industrialized production, high production cost, and low oosporin output, and improve cell concentration and product synthesis efficiency , the effect of increasing production

Inactive Publication Date: 2018-09-04
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the yield of oosporin in the above-mentioned fermentation research is still low, and its production cost is very high, far below the requirements of industrialized production

Method used

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  • Fermenting preparation method of oosporein
  • Fermenting preparation method of oosporein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Use 10 1000mL Erlenmeyer flasks, each containing 300mL medium A (glucose 3.5%, peptone 1.0%, soluble starch 3.0%, peanut meal 1.0%, corn meal 2.2%, soybean meal 0.2%, potato extract juice 1.5%, Magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.05%), sterilized at 120°C for 30 minutes, inoculated with activated Chaetomium cupreum (Chaetomium cupreum) CH-1 spore liquid after cooling, and cultured in shake flasks at 25°C for 42 hours. Inoculate the cultured strain solution into a 100L fermenter with 50L sterile medium B in a 10% inoculum, (medium B: 3.0% starch, 1.0% glycerol, 1.0% lactose, 2.5% sucrose, sulfuric acid Ammonium 0.05%, glucose 3.0%, peptone 1.5%, soybean powder hydrolyzate 1.0%, peanut cake powder 2.5%, soybean protein 2.0%, fermented soybean meal 1.0%, corn flour 1.5%, yeast powder 0.1%, dipotassium hydrogen phosphate 0.2 %, iron sulfate 0.01%, magnesium sulfate 0.01%, sodium chloride 0.1%), for fermentation production. After the strains were inocu...

Embodiment 2

[0053] Use 10 1000mL triangular flasks, each containing 300mL medium A (2.5% glucose, 1.5% peptone, 3.0% soluble starch, 1.5% peanut meal, 2.2% corn meal, 0.2% soybean meal, 1.5% potato extract juice, Magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.05%), sterilized at 120°C for 30 minutes, inoculated with activated Chaetomium cupreum (Chaetomium cupreum) CH-1 spore liquid after cooling, and cultured in shake flasks at 25°C for 42 hours. Inoculate the cultured strain solution into a 100L fermenter with 50L sterilized medium B in a 10% inoculum, (medium B: 3.0% starch, 1.0% glycerol, 2.0% lactose, 2.5% sucrose, sulfuric acid Ammonium 0.05%, glucose 4.0%, peptone 1.5%, soybean powder hydrolyzate 2.0%, peanut cake powder 2.5%, soybean protein 2.0%, fermented soybean meal 1.0%, corn flour 2.5%, yeast powder 0.1%, dipotassium hydrogen phosphate 0.2 %, iron sulfate 0.01%, magnesium sulfate 0.01%, sodium chloride 0.1%), for fermentation production. After the strains were in...

Embodiment 3

[0057]Use 10 1000mL Erlenmeyer flasks, each containing 300mL medium A (glucose 3.5%, peptone 1.0%, soluble starch 3.0%, peanut meal 1.0%, corn meal 2.2%, soybean meal 0.2%, potato extract juice 1.5%, Magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.05%), sterilized at 120°C for 30 minutes, inoculated with activated Chaetomium cupreum (Chaetomium cupreum) CH21-2-20 spore liquid after cooling, and cultured in shake flask at 25°C for 42 Hour. Inoculate the cultured strain solution into a 100L fermenter with 50L sterile medium B inside at an inoculum size of 10%, (medium B: starch 3.0%, glycerol 1.5%, lactose 2.5%, sucrose 2.5%, sulfuric acid Ammonium 0.05%, glucose 3.5%, peptone 1.5%, soybean powder hydrolyzate 2.5%, peanut cake powder 3.5%, soybean protein 3.5%, fermented soybean meal 1.0%, corn flour 1.5%, yeast powder 0.1%, dipotassium hydrogen phosphate 0.3 %, iron sulfate 0.01%, magnesium sulfate 0.01%, sodium chloride 0.2%), for fermentation production. After the...

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Abstract

The invention relates to a fermenting preparation method of oosporein. The method is characterized in that chaetomium cupreum and genetic improved strain CH-1 are utilized and processed by two-stage fermenting culture, and the culture medium is subjected to material supplementing and feeding, so that high carbon to nitrogen ratio in strain growing and product synthesizing can be maintained, and high-yield oosporein is obtained. The fermenting and extracting processes are simple; and the operation is convenient.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for preparing oosporin by fermentation. technical background [0002] Oosporein (Oosporein), also known as oosporin, is a diphenoquinone compound with a chemical formula of C 14 h 10 o 8 , whose chemical structure is shown in figure 1 ., is a physiologically active secondary metabolite produced by fungi such as soil fungi, entomogenous fungi, and basidiomycetes. It was named after Kogl. . [0003] In 1955, G.Lloyd et al. isolated oosporin from the fermentation broth of Chaetomium aureum; in 1959, P.V.Divekar et al. found that a fungal strain produced red substance, which was identified as Oosporin, the fungus is Acremonium (Acremonium SP.), and the acetylation and formylation of oosporin were also studied; in 1962, Vining L C K W J et al. Osporin was isolated from Beauveria Bassiana; in 1974, Richard J. et al. isolated a strain of Chaetomium tril...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/66C12N1/14C12R1/645
CPCC12N1/14C12P7/66C12N1/145C12R2001/645
Inventor 谭红钟娟周金燕杨杰舒丹罗笛
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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