311 results about "Isopentadiene" patented technology

Provided herein are methods for a robust production of isoprenoids via one or more biosynthetic pathways. Also provided herein are nucleic acids, enzymes, expression vectors, and genetically modified host cells for carrying out the subject methods. Also provided herein are fermentation methods for high productivity of isoprenoids from genetically modified host cells.

The present invention is directed to variant squalene synthase enzymes, including Saccharomyces cerevisiae squalene synthase enzymes, and to nucleic acid molecules encoding these variant enzymes. These variant enzymes produce squalene at a lower rate than the wild-type enzyme, allowing more farnesyl pyrophosphate to be utilized for production of isoprenoid compounds, while still producing sufficient squalene to allow the S. cerevisiae cells to grow without the requirement for supplementation by sterols such as ergosterol. These variant enzymes, therefore, are highly suitable for the efficient production of isoprenoids.

Provided herein are compositions and methods for improved production of acetyl-CoA and acetyl-CoA derived compounds in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a phosphoketolase (PK), and a functional disruption of an endogenous enzyme that converts acetyl phosphate to acetate. In some embodiments, the host cell further comprises a heterologous nucleotide sequence encoding a phosphotransacetylase (PTA). In some embodiments, the enzyme that converts acetyl phosphate to acetate is a glycerol-1-phosphatase. In some embodiments, the glycerol-1-phosphatase is GPP1/RHR2. In some embodiments, the glycerol-1-phosphatase is GPP2/HOR2. The compositions and methods described herein provide an efficient route for the heterologous production of acetyl-CoA-derived compounds, including but not limited to, isoprenoids, polyketides, and fatty acids.

The invention provides for methods for the production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in cells via the heterologous expression of phosphoketolase enzymes.

A non-toxic biologically active compound is provided having the following general formula (I):

wherein n is an integer from 1 to 14, preferably from 2 to 4, and R is an organic moiety, preferably a group different from but structurally substantially similar to 2-methyl naphthoquinone, or a group P—C(R₁)—P, where each P stands for a—PO(OH)₂ group and R₁ is a (poly)isoprenyl group, hydroxy, halogen (preferably chloro or bromo), or hydrogen, or a pharmaceutically acceptable derivative thereof. These compounds are useful for the treatment or prevention of certain disorders in a mammal, especially a human being, for example postmenopausal loss of bone in women, juvenile or senile osteoporosis in men and women, cardiovascular calcification and other ectopic calcifications.

The present invention relates to detergent compositions containing a specific blend of isoprenoid-based surfactants to deliver easy-rinse (low sudsing) cleaning performance.

Methods of treatment and prophylaxis of various diseases and disorders, and in particular diseases and disorders of lipid and bone metabolism, involving the administration of prenyl transferase (farnesyl pyrophosphate synthase) and/or isopentenyl pyrophosphate isomerase inhibitor compounds are disclosed.

Provided are methods of preparing polymers, such as polyisoprene, polybutadiene, polypiperylene, polycyclohexadiene, poly-β-farnesene, or poly-β-myrcene, using iron complexes. Also provided are novel iron complexes, pre-catalysts, intermediates, and ligands useful in the inventive polymerization system.

An automatic hot-melt lamp adhesive for plastic, metal and glass automobile lamps comprises the following components in percent by mass: 20-30% of styrene-isopentadiene-styrene block copolymer with styrene content of 14-16%, 2030% of styrene-ethylene-butene-styrene block copolymer with styrene content of 30-33%, 25-35% of tackifying resin, 5-10% of light calcium carbonate, 5-10% of thermoelectric blast furnace fly ash, 3-8% of process additive, 2-5% of plasticizer and 0.5-1% of antioxidant. The materials sequentially added into a horizontal kneading machine according to a proportion for heating and kneading, injected in a square mould for molding and encapsulated by using release paper after cooled to demould. The automatic hot-melt lam lamp adhesive has the advantages of high bonding strength, good vibration resistance, low cost and environmental protection, and the like.

In accordance with the present invention, a novel aromatic prenyltransferase, Orf2 from Streptomyces sp. strain CL190, involved in naphterpin biosynthesis has been identified and the structure thereof elucidated. This prenyltransferase catalyzes the formation of a C—C bond between a prenyl group and a compound containing an aromatic nucleus, and also displays C—O bond formation activity. Numerous crystallographic structures of the prenyltransferase have been solved and refined, e.g., (1) prenyltransferase complexed with a buffer molecule (TAPS), (2) prenyltransferase as a binary complex with geranyl diphosphate (GPP) and Mg²⁺, and prenyltransferase as ternary complexes with a non-hydrolyzable substrate analogue, geranyl S-thiolodiphosphate (GSPP) and either (3) 1,6-dihydroxynaphthalene (1,6-DHN), or (4) flaviolin (i.e., 2,5,7-trihydroxy-1,4-naphthoquinone, which is the oxidized product of 1,3,6,8-tetrahydroxynaphthalene (THN)). These structures have been solved and refined to 1.5 Å, 2.25 Å, 1.95 Å and 2.02 Å, respectively. This first structure of an aromatic prenyltransferase displays an unexpected and non-canonical (β/α)-barrel architecture. The complexes with both aromatic substrates and prenyl containing substrates and analogs delineate the active site and are consistent with a proposed electrophilic mechanism of prenyl group transfer. These structures also provide a mechanistic basis for understanding prenyl chain length determination and aromatic co-substrate recognition in this structurally unique family of aromatic prenyltransferases. This structural information is useful for predicting the aromatic prenyltransferase activity of proteins.

The invention provides a rare earth complex shown in formula (I) and a preparation method thereof and a rare earth catalyst system. The invention further provides a preparation method of butadiene-isoprene copolymer. The rare earth catalyst system is used for preparing the butadiene-isoprene copolymer. The rare earth catalyst system comprises alkyl benzene sulfonic acid rare earth complex and aluminum alkyl, so that the catalyst system does not contain halogen and does not have a corrosion action, and is environment friendly during the process of preparing the butadiene-isoprene copolymer. Experimental results show that the content of cis-1,4 in butadiene and isoprene of the butadiene-isoprene copolymer prepared by the rare earth catalyst system is larger than 95%.

The invention discloses a nitrogenous functionalized rare earth styrene/isoprene/butadiene copolymer and a preparation method thereof. The weight-average molecular weight of the copolymer is 1*10<4>-120*10<4>; by 100% of total quantity of the copolymer, the mass percent of sum of bound styrene content and styrene derivative content is 5%-60%, the mass percent of butadiene content is 10%-70%, and the mass percent of isoprene content is 10%-70%; by 100% of sum of bound styrene content and styrene derivative content, the mass percent of bound styrene content is less than 100%, and the rest is a styrene derivative; by 100% of total quantity of polyisoprene, the mass percent of 1, 4-polyisoprene content is 70%-98%; by 100% of total quantity of polybutadiene, the mass percent of 1, 4-polyisoprene content is 70%-98%; the styrene derivative is selected from nitrogen-atom substituent group-containing styrene and at least contains one tertiary amine group substituent group; the substituent group can be directly connected to the ortho-position, meta-position or para-position of the styrene.

Disclosed is a carboxyl group-containing polyurethane (A) produced from a polyol (b) containing at least 10 mol% (relative to the total (100 mol%) of the polyol (b)) of a polyol (b1) which: (i) has a number-average molecular weight of 500 to 50,000; (ii) has 1 to 10 hydroxyl groups per molecule; and (iii) is one or a combination of two or more polyols selected from the group consisting of polybutadiene polyol, polyisoprene polyol, hydrogenated polybutadiene polyol and hydrogenated polyisoprene polyol. The carboxyl group-containing polyurethane (A) is suitable as materials of cured products, for example cured films, that are excellent in adhesion with substrates, low warpage, flexibility, plating resistance, soldering heat resistance and long-term reliability.

The invention provides an improved perovskite type catalyst which has a structure as shown in a formula (I): ABO3/X(I), wherein A is selected from one or more of La, Ce, Gd, Pr, Nd, Ca, Mg, Sr and Ba;B is selected from one or more of Ti, V, Cr, Cu, Fe, Mn, Ag, Nb, W and Mo; and X is selected from one or more of boron, phosphorus and sulfur. The ABO3 atomic structures in the catalyst have the characteristic of high thermal stability; structure and composition are regulated in a diverse manner by means of doping and/or close atomic substitution; by then selecting special doped improved components, the perovskite type catalyst with the ABO3/X structure is obtained. The water resistance of the catalyst is improved, the loss rate of active components of the catalyst is improved, the service life of the catalyst is prolonged, corresponding production cost is lowered, and a foundation is laid for industrial continuous long-term production.

A mouth guard which is excellent in impact absorbability and durability, is thin and has good fittability, and is able to easily chew thereupon, and does not give vomiting feel; and a sheet for mouth guard which is suitable for producing the mouth guard. The mouth guard or the sheet for mouth guard comprises at least one block copolymer selected from the group consisting of a block copolymer (a-1) comprising polymer blocks A made of a vinyl aromatic compound, and polyisoprene blocks B having a content of 1,2-bonds and 3,4-bonds of 40% by mol or less; a block copolymer (a-2) comprising polymer blocks A, and polymer blocks C which contain an isoprene unit and a butadiene unit in a ratio of from 5/95 to 95/5 (% by weight) and have a content of 1,2-bonds and 3,4-bonds of 40% by mol or less; and a block copolymer (a-3) comprising polymer blocks A, and polybutadiene blocks D having a content of 1,2-bonds of 60% by mol or more, wherein a content of vinyl aromatic compound units in each of the copolymers is from 10 to 40% by weight, and a hydrogenated product thereof (b-1) to (b-3).