Fungus with straw degradation capability and application thereof in composting

A straw and fungus technology, applied in the fields of application, fungi, organic fertilizers, etc., can solve the problems of inability to degrade pectin and hemicellulose components, achieve good application value, accelerate degradation speed, and increase organic matter content.

Inactive Publication Date: 2013-12-25
SHANGHAI JIAO TONG UNIV
View PDF4 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this technology cannot achieve the degrada

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fungus with straw degradation capability and application thereof in composting
  • Fungus with straw degradation capability and application thereof in composting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Isolation, purification and preservation of strains

[0024] The rotten straw and soil of the paddy field of Pujiang Base of Jiaotong University, Pujiang Town, Minhang District, Shanghai were taken as samples. Shake flask enrichment culture was adopted, and 10 g of different samples were ground and mixed, placed in 100 mL enrichment medium, and incubated at 30°C and 150 rpm. Every 5 days, 10% of the inoculum was transferred to another enriched medium, and the acclimatization was repeated 4 times. The enrichment medium adopts the medium with CMC-Na as the sole carbon source.

[0025] Take 100 μL of the enrichment solution and spread it on the CMC-Congo red medium plate, place it in a constant temperature and humidity incubator at 28°C and culture it upside down, and observe after 5 days. Pick the bacteria with a larger diameter of the hydrolysis circle and inoculate them on the PDA medium until a single colony is obtained. Then the single bacterium colony obtained is ...

Embodiment 2

[0028] Identification of Penicillium oxalicum strains

[0029] Combined with physiological and biochemical identification and 18S rDNA ITS sequence comparison analysis ( figure 2 shown), the bacterium was identified as Penicillium oxalicum, and the taxonomic species name was Penicillium oxalicum.

[0030] The strain was named JSD-17, and it was preserved in the General Microbiology Center (CGMCC) of the China Committee for the Collection of Microbial Cultures (CGMCC) on June 19, 2013. Address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China Science Microbiology Research Institute, the deposit number is CGMCC No.7743.

Embodiment 3

[0032] Determination of Enzyme Production Activity of Penicillium oxalicum

[0033] Using the DNS method, the activities of the two cellulase CMCase and FPase in the optimized fermentation broth were determined to be 72.69U / mL and 40.55U / mL respectively; the activities of pectinase and xylanase were determined to be 5.86U / mL and 3.26U / mL. U / mL. Wherein, one enzyme activity unit (U) is the amount of enzyme that generates 1 μg of monosaccharide product per milliliter of enzyme liquid catalyzed reactants per minute.

[0034] ABTS, veratrol, Mn 2+ As the reaction substrate, the activities of laccase (Lac), lignin peroxidase (Lip) and manganese peroxidase (Mnp) in the fermentation broth were determined by spectrophotometry. The activities of the three enzymes were determined to be 15.8U / L, 1.94U / L, and 5.54U / L, respectively. It is defined here that 1 μmol of substrates (ABTS, veratrol, Mn 2+ ) is an enzyme activity unit (U).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a fungus with straw degradation capability and application thereof in composting and relates to the technical field of biological environmental protection. The straw degradation fungus JSD-17 is penicillium oxalicum (Penicillium oxalicum) and has a preservation number of CGMCC No.7743; the straw degradation fungus is further used for fermenting and composting agricultural waste straw. The penicillium oxalicum disclosed by the invention not only has strong cellulose enzyme synthesis capability, but also has the capability of decomposing hemicellulose and pectin components in the straw. By adopting the fungus disclosed by the invention, laccase (Lac), lignin peroxidase (Lip) and manganese peroxidase (Mnp) can be synthesized; the degradation speed of lignin components in the straw can be greatly increased.

Description

technical field [0001] The invention relates to a method in the technical field of biological environmental protection, in particular to a fungus with straw degrading ability and its application in composting. Background technique [0002] my country is a large agricultural country, and the annual output of various crop straws is about 700 million tons. Since the late 1990s, with the increase in crop yields, the total amount of straw has increased rapidly. In order to catch up with the farming season, rush to sow seeds, and save trouble, the majority of farmers have begun to burn straw in most areas. Large-scale straw burning not only causes great environmental pollution, but also wastes precious resources. [0003] The main components of straw are cellulose, lignin, pectin, hemicellulose, crude protein and ash. The structure of cellulose, pectin, especially lignin in straw is very stable, and it degrades very slowly in nature. Microorganisms in the environment have the c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/14C05F17/00C05F11/08C12R1/80
CPCY02W30/40
Inventor 周培冯海玮支月娥罗艳青卫星毛亮
Owner SHANGHAI JIAO TONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap