46 results about "Rhoifolin" patented technology

The present invention mainly relates to a preparation method of Mao pomelo peel total flavone, Mao pomelo peel total flavone dispersing tablet, naringoside and rhoifolin and their application. The pomelo peel is a high-grade Chinese medicinal material for relieving cough and resolving phlegm. The flavone component in the Mao pomelo peel is its main effective component, in the Mao powder peel total flavone the naringoside and rhoifolin contents are higher, and are above 85% of the total flavone. Said invention provides a solvent method to make extraction, and makes the extract undergo the processes of concentration, precipitation and recrystallization so as to obtain the above-mentioned products.

The invention provides medicinal exocarpium tea and a process for preparing the same. By the aid of the medicinal exocarpium tea and the process, problems that single exocarpium or berry tea is not suitable to be drunk for a long term and the like can be solved, and rhoifolin in exocarpium and dihydromyricetin in berry tea can be sufficiently utilized. The process includes steps of flowery-flavor concentrated liquid extraction, exocarpium extract extraction, aroma enhancement, mixing, packaging and the like to obtain a tea product. The medicinal exocarpium tea and the process have the advantages that functional ingredients of exocarpium tea and the berry tea can be sufficiently utilized, the medicinal exocarpium tea is suitable to be drunk by people for a long term and has good taste, and tea soup is good in color and luster; owing to heating aroma enhancement modes, the medicinal exocarpium tea is also suitable to be drunk by people who suffer from pollen allergy, accordingly, the service range of scented tea can be expanded, and the medicinal exocarpium tea and the process are favorable for integration and healthy development of various fresh flower and tea industries; combined merits of the traditional tea and instant tea can be sufficiently utilized, the dissolution speed of first-brewing can be particularly increased, and the quality of the product can be improved.

The invention discloses a UPLC fingerprint spectrum detection method of xuefu zhuyu decoction and capsules, and belongs to the field of column chromatography. According to the detection method, ultra-high performance liquid chromatography analysis is conducted on contents of xuefu zhuyu powder or capsules and a reference substance solution. Chromatographic conditions are that gradient elution is conducted by taking acetonitrile as a mobile phase A and taking a 0.1% formic acid aqueous solution as a mobile phase B, the detection wavelength is 280 nm, the column temperature ranges from 30 DEG C to 50 DEG C, the flow velocity ranges from 0.3 mL.min<-1> to 0.5 mL.min<-1>, and the injection volume is 2 microlitres. Fingerprint spectrum information of the xuefu zhuyu decoction and capsules is analyzed, 30 common peaks are totally obtained, the relative retention time and relative peak area of the common peaks are calculated by taking rhoifolin as a reference peak, and 16 common peaks are identified through a standard substance comparison method. The UPLC fingerprint spectrum detection method of the xuefu zhuyu decoction and capsules is comprehensive, rapid and reliable, fingerprint spectrum information of multiple components can be comprehensively obtained by means of the method, and therefore a reference basis is provided for quality control of the xuefu zhuyu decoction and capsules.

The invention relates to a rhoifolin and a prepared medicament application thereof. The method for extracting the rhoifolin comprises the following steps of: taking turpinia formosana leaves or exocarpium citrl grandis, adding 5-15 times of 30-90 percent ethanol for carrying out refluxing extraction for 1-3 times and filtering, wherein each refluxing extraction is carried out for 1-3 hours; combining filter liquid and recycling ethanol; eluting an aqueous solution by a macroporous resin column, collecting an eluted part, depressurizing for concentrating and drying to obtain the mixed total glycoside of the rhoifolin; and separating, combining rhoifolin fractions and crystallizing to obtain pure rhoifolin. The rhoifolin can be used as a neuraminidase inhibitor for preventing and treating influenza and made into a pharmaceutically acceptable formulation.

The invention belongs to the field of pharmacy, and provides application of three flavone glycoside compounds luteolin-7-O-[alpha-L-rhamnose-(1->2)]-beta-D-glucoside (trivial name is lonicerin), apigenin-7-O-[alpha-L-rhamnose-(1->2)]-beta-D-glucoside (trivial name is rhoifolin) and luteolin-6-C-beta-D-glucose-8-C-beta-D-xyloside in preparing medicament or health-care food for preventing and treating hepatitis.

The invention discloses a method for extracting rhoifolin from turpinia formosana leaves. The method comprises the following steps of: crushing the turpinia formosana leaves serving as a raw material into 40 to 80 meshes, adding the powder into a supercritical extraction kettle, introducing liquid CO2 and entrainer, extracting for 2 to 3 hours under the pressure of 20 to 30MPa and at the temperature of between 40 and 50 DEG C, regularly resolving the extract, reclaiming the reagent, dissolving the obtained extract into hot water, adding active carbon for decolorization, adding polyamide resin for adsorption into the decolorized solution, performing gradient elution by using an aqueous solution of ethanol, collecting the target component, concentrating under reduced pressure, crystallizing the concentrate, filtering the crystal, dissolving the crystal into the hot water, performing re-crystallization, drying, and thus obtaining the rhoifolin. The method for producing the rhoifolin is low in energy consumption, low in pollution, simple in steps and high in purity of the obtained product, and is suitable for industrialized production.

The invention relates to an acute turpinia leaf general flavone ethanol reflux extract, which is prepared from acute turpinia leaves through ethanol reflux extraction, wherein an extraction method comprises the following steps: taking acute turpinia leaves; adding 30 to 90 percent of ethanol accounting for 5 to 15 times of the volume for carrying out reflux extraction for 1 to 3 times, wherein each time of reflux extraction lasts for 1 to 3 hours; carrying out filtration; merging filter liquid; recovering the ethanol from the filter liquid; using nonpolar macroporous absorbent resin for absorbing water solution; using the ethanol lower than 20 percent for elution to remove impurities; then, using 25 to 90 percent of ethanol for elution; collecting elution liquid; recovering the ethanol; and carrying out concentration and drying to obtain the acute turpinia leaf general flavone ethanol reflux extract. The general flavone content is higher than 50 percent, and the flavone type major chemical ingredients are one kind or several kinds of materials in nuezhenoside, rhoifolin, hyperin, meletin, cyanidenon, apiolin and glucoside or aglycon thereof. The extract can be used as a neuraminidase inhibitor to be used for preventing and treating flu, and can be made into pharmaceutically acceptable preparations.

The invention relates to rhoifolin and usage of a drug prepared by the rhoifolin. The rhoifolin extraction method includes the steps: taking folium turpiniae or pummelo peel, adding 5-15 times of amount of ethanol with the concentration of 30%-90%, performing reflux extraction for 1-3 times, each reflux extraction takes 1-3 hours and filtering; and combining filtrate, recovering the ethanol, eluting water solution by macroporous resin, collecting eluted portions, depressurizing, concentrating and drying to obtain mixed total glycoside of the rhoifolin prior to separation, combining rhoifolin fluid parts, crystallizing and obtaining a rhoifolin pure product. The rhoifolin can serve as neuraminidase inhibitor to prevent and treat influenza, and can be made into dosage forms acceptable in pharmacy.

The invention provides a method for extracting and separating volatile oil and flavonoid from pummelo pee leaves. The method comprises the following steps: crushing and sieving the pummelo pee leaves,so as to obtain powder, and uniformly filling a powder wet sample into a glass chromatographic column; firstly extracting volatile oil with low polarity with a petroleum ether and ethanol mixed solvent with low polarity according to a like dissolves like principle, then extracting flavonoid with solvent ethanol aqueous solution with medium polarity, combining extracting solution, leaving the solution to stand for layering, and ensuring that an upper layer contains the volatile oil and the lower layer contains two types of flavone which are naringin and rhoifolin. According to the method, twomain types of substance are continuously extracted, and target products are respectively dissolved in two liquid phases because petroleum ether is automatically layered in a condition of the existenceof water, so that the purpose of separation is achieved. The method is simple in technical process, small in amount of used solvent and high in extraction efficiency, and can sufficiently use leavesusually used as waste to produce a large amount of volatile oil and flavonoid.

The invention discloses a method for purifying Rhododendron, which is characterized in that: the round leaves of Rhizoma chinensis are used as raw materials, crushed, and 55-70% ethanol is added for ultrasonic extraction 1-3 times, the extract is concentrated under reduced pressure, and the concentrated solution is adjusted to pH 3- 4. Filtrate, add polyamide resin to the filtrate for adsorption, elute with ethanol water, concentrate the collected solution to recover ethanol, filter the concentrated solution through a high-speed centrifuge, and separate the filtrate through a membrane separator. The obtained components are separated by high-speed countercurrent chromatography and detected by an ultraviolet detector , collect the target components, and dry under reduced pressure to obtain Rhodorin. The method of the invention has the advantages of simple operation, short production period, large preparation amount and high efficiency, and is suitable for the preparation of high-purity rhodium.

The invention discloses a method for preparing two chemical reference substances of Rhoifolin and naringin simultaneously. The method comprises the following steps of simultaneously extracting Rhoifolin and naringin in exocarpium leaves with an ethanol solution, after vacuum concentration of an extracting solution, extracting with an n-butyl alcohol-petroleum ether mixed solution to remove chromophyll, putting the lower part in a refrigerator for standing, washing with cold water and centrifuging, removing impurities such as water-soluble polysaccharide and proteins, dissolving a crude product with methyl alcohol, then using a high performance liquid preparative chromatograph for separation, and thus obtaining the two reference substances of Rhoifolin and naringin with the purity of more than 98%. According to the method, the reference substances of Rhoifolin and naringin can be prepared simultaneously, the processing step is simple, the purity is high, the cost is low, and the preparation quantity is large.

The invention relates to a nuezhenoside-rhoifolin-hyperin composite, in particular to a composite which is prepared from the following components by weight percent: 80%-40% of nuezhenoside, 5%-45% of rhoifolin and 1%-40% of hyperin. The composite can be used as neuraminidase inhibitor in the prevention and treatment of influenza and be used to prepare pharmaceutically acceptable dosage forms.

The invention relates to a nuezhenoside and rhoifolin composition, which is prepared by mixing 20%-80% by weight of nuezhenoside with 80%-20% by weight of rhoifolin, the composition can be used for preventing and treating influenza as a neuraminidase inhibitor and be prepared into pharmaceutically acceptable formulations.

The invention discloses a method for synthesizing tilianin from naringin as a raw material. The method comprises the following steps: S1, synthesizing rhoifolin; S2, synthesizing a compound, namely 5,7-dyhydroxy-2-(4-methoxy-phenyl)-4H-chromene-4-ketone; S3, synthesizing acetoxyl methyl)-6-((5-hydroxy-2-(4-methoxy-phenyl)-4-oxo-4H-chromene-7-yl) alkoxy) tetrahydro-2H-pyran-3,4,5-triester triacetate; S4, synthesizing a compound, namely 5-hydroxy-2-(4-methoxy-phenyl)-7-(((2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl) tetrahydro-2H-pyran-2-yl-yl) alkoxy)-4H-benzopyran-4-ketone. By adopting the method, the naringin is adopted as the raw material to synthesize the tilianin through reactions such as oxidation, methylation and hydrolysis.

The invention relates to a method for extracting rhoifolin and toxicodendron succedaneum wax from toxicodendron succedaneum. The method comprises the following steps: extracting dried toxicodendron succedaneum with petroleum ether; refluxing and extracting filter residues with a dilute acid alcohol solution; filtering, and concentrating to dry; dissolving with acid water; performing resin absorption, stepwise elution, concentration and crystallization to obtain purified products of rhoifolin and toxicodendron succedaneum wax. The method provided by the invention is simple in operation, greatly increases yield of rhoifolin and toxicodendron succedaneum wax, greatly reduces production costs of rhoifolin and toxicodendron succedaneum wax, simplifies production processes, and can also obtain large quantities of oily substances; the toxicodendron succedaneum is used as an extraction material to not only improve utilization efficiency of the toxicodendron succedaneum, but also increase an added value of the toxicodendron succedaneum.

The invention discloses a method for preparing naringenin and apigenin by converting exocarpium citrus grandis leaf flavone by using microorganism. The method comprises the steps of making bacteria and mold naturally growing in exocarpium citrus grandis material into suspension liquid, inoculating the suspension liquid into a potato liquid medium for culturing to acquire seed bacterium culturing solution, inoculating seed bacteria into a conversion medium for culturing, and performing high performance liquid chromatography. A result shows that the quantities of the naringenin and the apigenin are improved above ten times; and the pure naringenin and apigenin are respectively acquired by performing organic solvent extraction and macroporous resin separation and purification on conversion liquid. According to the method provided by the invention, respective extraction of naringin and rhoifolin is not needed, and the microorganism is directly used for converting naringin and rhoifolin substrates synchronously existing in exocarpium citrus grandis leaves. The method is simple and easy to operated, a pollution problem caused by acid hydrolysis is also avoided; as the strain is taken from elements naturally growing on exocarpium citrus grandis branches or fruits, so that the method has the advantages of strong selectivity and high conversion rate, and purities of the naringenin and apigenin prepared according to the method can be more than 98%.

The invention provides a method for extracting and purifying fustin and rhoifolin from rhus punjabensis var. sinica rehd seeds. The method comprises the following steps: performing enzyme and microbial fermentation treatment, performing reflux extraction by adopting alkali alcohol water, filtering, concentrating till being dry, dissolving in alcohol water and then pouring onto a macroporous resin column, crystallizing under high temperature and re-crystallizing, thereby acquiring the pure products of fustin and rhoifolin. Through an enzyme method and alkali extraction, the extraction ratio of fustin and rhoifolin is greatly increased and the influence of the protein in the rhus punjabensis var. sinica rehd on the extraction is reduced. The combination of column chromatography and crystallization is adopted for separating, so that the purity of fustin and rhoifolin is greatly increased, the operation steps are simplified and the production cost of the fustin and rhoifolin is reduced.

The invention belongs to the field of pharmacy, and provides application of three flavone glycoside compounds luteolin-7-O-[alpha-L-rhamnose-(1->2)]-beta-D-glucoside (trivial name is lonicerin), apigenin-7-O-[alpha-L-rhamnose-(1->2)]-beta-D-glucoside (trivial name is rhoifolin) and luteolin-6-C-beta-D-glucose-8-C-beta-D-xyloside in preparing medicament or health-care food for preventing and treating hepatitis.

The invention belongs to the field of medicine and relates to a composition of nuezhenoside, rhoifolin and hyperoside. Specially, the composition comprises, by weight, 40-90% of the nuezhenoside, 5-40% of the rhoifolin and 5-30% of the hyperoside, and the composition has good treatment and / or prevention effects on neurodegenerative diseases and can overcome side effect defects of existing medicine.

The invention discloses scented tea with the flavor of exocarpium citri rubrum and siraitia grosvenorii and a preparing method of the scented tea. The scented tea is prepared from, by weight, 20-35 parts of slices of exocarpium citri rubrum, 45-80 parts of seeds of siraitia grosvenorii and 0-30 parts of freeze-drying flowers of exocarpium citri rubrum. The slices of exocarpium citri rubrum are obtained by conducting steam re-moisturizing on dried fruits of slices of exocarpium citri rubrum, slicing, atomizing, adding hydromel, and conducting low-temperature vacuum drying; the flowers of exocarpium citri rubrum are subjected to freeze drying to be prepared into the reeze-drying flowers of exocarpium citri rubrum; the obtained slices of exocarpium citri rubrum are mixed with the freeze-drying flowers of exocarpium citri rubrum and the seeds of siraitia grosvenorii to obtain the scented tea with the flavor of exocarpium citri rubrum and siraitia grosvenorii. By means of the scented tea with the flavor of exocarpium citri rubrum and siraitia grosvenorii and the preparing method of the scented tea, the effective components like general flavone of exocarpium citri rubrum, naringin and rhoifolin can be effectively stored, and the hydromel is utilized to cover up the bitter flavor of exocarpium citri rubrum and improve the mouthfeel; the scented tea of exocarpium citri rubrum is yellowish in soup color, and limpid and bright, has the sweetness of siraitia grosvenorii and the fragrance of the flowers of exocarpium citri rubrum, is pure in mouthfeel, and quickly becomes sweet after taste. The scented tea has the functions of reliving internal heat, moistening the lung, lubricating the intestines, relaxing the bowels, and relieving cough and reducing sputum, can be used for of relieving internal heat, and removing symptoms like cough due to wind-cold evil, itchy laryngeal and excessive phlegm, dyspepsia and sickness from excessive drinking amount, and vomiting and stuffiness,and the scented tea has high application and popularization value.

The invention relates to an acute turpinia leaf general flavone ethanol reflux extract, which is prepared from acute turpinia through ethanol percolation extraction. A percolation extraction method comprises the following steps: taking acute turpinia leaf coarse powder; adding 30 to 90 percent of ethanol as extracting agents; carrying out soaking for 12 to 72 hours; using the speed of 1 to 8 ml / kg / minute for percolation; collecting percolation liquid accounting for 4 to 15 times of the amount of medical materials; recovering the ethanol; using nonpolar macroporous absorbent resin for absorbing water solution; using the ethanol lower than 20 percent for elution to remove impurities; then, using 25 to 90 percent of ethanol for elution; collecting elution liquid; recovering the ethanol; and carrying out concentration and drying to obtain the acute turpinia leaf general flavone ethanol reflux extract. The general flavone content is higher than 50 percent, and the flavone type major chemical ingredients are one kind or several kinds of materials in nuezhenoside, rhoifolin, hyperin, meletin, cyanidenon, apiolin and glucoside or aglycon thereof. The extract can be used as a neuraminidase inhibitor to be used for preventing and treating flu, and can be made into pharmaceutically acceptable preparations.

The invention discloses dried citrus reticulate blanco outer bark and jasminum tea. The dried citrus reticulate blanco outer bark and jasminum tea consists of the following components in parts by weight: 15-35 parts of dried citrus reticulate blanco outer bark slices, 20-40 parts of tender leaves of hydrangea strigosa or h.angustipetala gayata, and 40-55 parts of jasminum. A preparation method ofthe dried citrus reticulate blanco outer bark and jasminum tea comprises the following steps of sequentially enabling the dried citrus reticulate blanco outer bark to be wet with steam, performing dicing, performing atomization with hydromel, and performing low-temperature vacuum drying so as to obtain the dried citrus reticulate blanco outer bark slices; and mixing the dried citrus reticulate blanco outer bark slices with the tender leaves of hydrangea strigosa or h.angustipetala gayata so as to obtain the dried citrus reticulate blanco outer bark and jasminum tea. According to the dried citrus reticulate blanco outer bark and jasminum tea, effective components of dried citrus reticulate blanco outer bark total flavonoids, naringin and rhoifolin can be effectively preserved, the tea soupis pure, clear and bright in color and has the fragrance of the jasmine, the bitterness of the dried citrus reticulate blanco outer bark can be improved, and the mouth feel is pure and sweet after taste. The dried citrus reticulate blanco outer bark and jasminum tea has the health-care efficacy of invigorating the spleen, regulating vital energy, clearing heat, removing toxicity, soothing the nerves, relieving cough, reducing sputum and nourishing the throat.

The invention relates to a rhoifolin and hyperin composition which is a composition prepared from 20%-80% of rhoifolin and 80%-20% of hyperin in percentage by weight. The composition can be used as a neuraminidase inhibitor for preventing and treating flu and can be made into pharmaceutically acceptable formulations.

The invention relates to the technical field of medicinal chemistry, and particularly discloses an exocarpium citri grandis extract and application thereof to preparation of an anti-cancer drug. The extract is prepared from the following active ingredients in percentage by weight: 30% to 40% of nobiletin, 20% to 30% of kaempferol and 20% to 30% of rhoifolin. The exocarpium citri grandis extract disclosed by the invention is definite in active ingredient and content and obvious in anti-hepatoma effect.

The invention provides a method for preventing and controlling pomelo tree planting insect pests, which belongs to the field of plant disease and insect pest control, and comprises the following steps: before the beetle lays eggs, mix the soil with trichlorfon and cultivate the soil: mix the trichlorfon with the topsoil, cultivate it on the base of the trunk 5 cm, and then Conical soil, the height is 20cm, and the bottom radius is 30cm. After the beetle lays eggs, the eggs are manually scraped off and the larvae are killed by hooking. 30 days before the harvest of Huajuhong, inject 1.5mL of imidacloprid liquid per cm trunk diameter. The invention can improve the spawning position of the star beetle and make the distribution of the eggs relatively concentrated, which reduces the difficulty of manually scraping the eggs and killing the larvae, and improves the control efficiency at the same time, and the pesticide residues in the fruit are always within the safe range. There was no significant effect on the active ingredients of citrus red (naringin, anacardin).

The invention discloses a method for simultaneously separating, purifying and preparing plurality of flavonoid substances by utilizing an industrial chromatographic technology. The purity of nuezhenoside and rhoifolin monomers prepared at the same time is 99.5% or above. According to the method, real-time online monitoring of a separation process can be realized; the method is good in process reproducibility, easy to operate, short in preparation time, small in organic solvent dosage, capable of achieving large-scale industrial production, capable of preparing two reference substances at the same time, capable of improving the utilization rate of medicinal materials and environmentally friendly.

The invention relates to the technical field of medicinal chemistry, and particularly discloses an exocarpium citri grandis extract and application thereof to preparation of an anti-cancer drug. The extract is prepared from the following active ingredients in percentage by weight: 30% to 40% of nobiletin, 20% to 30% of kaempferol and 20% to 30% of rhoifolin. The exocarpium citri grandis extract disclosed by the invention is definite in active ingredient and content and obvious in anti-hepatoma effect.

The invention relates to application of rhoifolin-hyperin composition in preparing medicine for treating and / or preventing neurodegenerative diseases.

The invention discloses an application of rhoifolin in preparation of a drug for preventing and treating osteolytic diseases. In-vitro experiments show that the rhoifolin can significantly inhibit theosteoclast formation and bone absorption induced by RANKL and also inhibit the expression of osteoclast-related genes. Western blotting experiments show that the rhoifolin can inhibit NF-[kappa]B andMAPK signaling pathways and two transcription factors of NFATc1 and c-fos; further studies show that the rhoifolin can inhibit P65 from entering nucleus and the activity of NFATc1 and NF-[kappa]B cells. In-vivo experiments show that the rhoifolin can reduce the number of TRAP-positive osteoclasts and bone destruction of skull in a titanium particle-induced skull model. Therefore, the rhoifolin can improve osteolysis induced by osteoclasts, and can become a potential drug for treatment of joint prosthesis loosening, and provides a new treatment option for aseptic loosening of prosthesis.

The invention discloses a biodegradable color printing ink auxiliary which comprises the following main raw materials in parts by weight: 5-10 parts of menthol, 2-6 parts of glaucocalyxin, 17-22 parts of hasmanine, 20-30 parts of 13-dehydroxyindaconintine, 8-17 parts of gomisin G, 15-25 parts of rhoifolin, 11-14 parts of scopolin, 5-9 parts of 2-chlorin-6-fluorophenol, 7-12 parts of chloromethyl methyl ether, 0.5-0.8 part of boron trifluoride methyl alcohol, 2-4 parts of pentobarbital sodium salt, 2-4 parts of 2-aminophenol-4-(2-carboxyl) sulphonanilid and 2-4 parts of cis-dichloride (diethyl sulfide) platinum. The biodegradable color printing ink auxiliary has the advantages of environmental protection, harmless effect, high safety, stable property and good printing effect. When the wastes are recycled, under the effect of a decolorizing agent, the ink can be quickly decolorized, the problem of volatilization of the organic solvent in the oil-based ink can be eliminated and the problems of high deinking difficulty of wastepaper and serious sewage discharge can be solved.