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Method for simultaneously separating, purifying and preparing plurality of flavonoid substances by utilizing industrial chromatographic technology

A technology of simultaneous separation and industrial chromatography, applied in the field of separation and purification of active components of traditional Chinese medicine, can solve problems such as undiscovered, and achieve the effects of short production cycle, cost saving, and improved utilization of medicinal materials

Pending Publication Date: 2021-06-29
JIANGZI QINGFENG PHARMACEUTICALS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no prior art for the simultaneous preparation of privetin and rhoiferin reference substances

Method used

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  • Method for simultaneously separating, purifying and preparing plurality of flavonoid substances by utilizing industrial chromatographic technology
  • Method for simultaneously separating, purifying and preparing plurality of flavonoid substances by utilizing industrial chromatographic technology
  • Method for simultaneously separating, purifying and preparing plurality of flavonoid substances by utilizing industrial chromatographic technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: the preparation of the extract of the round leaf of Shanxiang

[0030] Take an appropriate amount of round leaves of Shanxiang, add 10 times the volume of 70% ethanol to reflux for extraction twice, each time for reflux extraction for 1 hour, and filter; combine the filtrates, recover ethanol from the filtrates, and absorb the aqueous solution with a non-polar macroporous adsorption resin, and use 20% The following ethanol (such as 18%) elutes to remove impurities, and then elutes with 70% ethanol, collects the eluate, reclaims ethanol, concentrates, and dries to obtain the extract of Rhizoma chinensis, wherein the content of nyguletin is about 8.0% ( W / W), the Rhodorin content is about 6.0% (W / W) see figure 1 .

Embodiment 2

[0031] Embodiment 2: the preparation of the extract of the round leaf of Shanxiang

[0032] Take an appropriate amount of round leaves of Shanxiang, add 5 times the volume of 30% ethanol to reflux and extract once, each time reflux extracts for 1 hour, and filter; combine the filtrate, recover ethanol from the filtrate, and absorb the aqueous solution with a non-polar macroporous adsorption resin, and use 20% The following ethanol (such as 15%) elutes to remove impurities, and then elutes with 25% ethanol, collects the eluent, reclaims ethanol, concentrates, and dries to obtain the extract of Rhizoma chinensis, wherein the content of privetin is about 2.0% ( W / W), the content of Rhodorin is about 1.0% (W / W).

Embodiment 3

[0033] Embodiment 3: the preparation of the extract of the round leaf of Shanxiang

[0034] Take an appropriate amount of round leaves of Shanxiang, add 15 times the volume of 90% ethanol to reflux and extract twice, each reflux extracts for 2 hours, and filter; combine the filtrate, recycle the ethanol from the filtrate, and absorb the aqueous solution with a non-polar macroporous adsorption resin, and use 20% The following ethanol (such as 10%) elutes to remove impurities, and then elutes with 90% ethanol, collects the eluate, reclaims ethanol, concentrates, and dries to obtain the extract of Rhizoma chinensis, wherein the content of privetin is about 6.0% ( W / W), rhozolin content is about 3.0% (W / W).

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Abstract

The invention discloses a method for simultaneously separating, purifying and preparing plurality of flavonoid substances by utilizing an industrial chromatographic technology. The purity of nuezhenoside and rhoifolin monomers prepared at the same time is 99.5% or above. According to the method, real-time online monitoring of a separation process can be realized; the method is good in process reproducibility, easy to operate, short in preparation time, small in organic solvent dosage, capable of achieving large-scale industrial production, capable of preparing two reference substances at the same time, capable of improving the utilization rate of medicinal materials and environmentally friendly.

Description

technical field [0001] The invention belongs to the technical field of separation and purification of active ingredients of traditional Chinese medicines, and in particular relates to a method for simultaneously separating and purifying various flavonoids by using industrial chromatographic technology. Background technique [0002] Ligustinoside is a flavonoid glycoside compound, which is one of the main active ingredients of Ligustrum lucidum and Cymbidium round leaves, and has the effect of inhibiting influenza virus infection and replication and inhibiting neuraminidase. Rhodoglucoside is a flavonoid glycoside compound, and it is one of the main active ingredients of the round leaves of Cymbidium sinensis and the fruits of Rutaceae plants (such as orange red, etc.), and has anti-oxidation, anti-tumor, anti-hypertensive and xanthine oxidase inhibitory effects. [0003] At present, the control of the quality of medicinal materials, the screening of pharmacological effects, ...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08C07H17/07
CPCC07H17/04C07H1/08C07H17/07
Inventor 刘地发王章伟黄冰峰古福生徐滢王振周鹏刘尧奇张功俊钟仁清李仁政
Owner JIANGZI QINGFENG PHARMACEUTICALS INC
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