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A kind of purification method of Rhodorin

A technique of anacardoside and purification method, applied in the field of natural medicinal chemistry, can solve the problems of low industrialization, large equipment investment, high cost of solvent extraction, and achieve the effects of less organic residues, high separation ability and high purity

Inactive Publication Date: 2011-12-21
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing production processes of flavonoid glycosides mainly include solvent extraction, supercritical extraction and resin method, among which the cost of solvent extraction is high, the investment in supercritical extraction equipment is large, and the current degree of industrialization is low

Method used

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  • A kind of purification method of Rhodorin

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take 1kg of the whole herb of Rhizoma chinensis, crush it, add 7 times the amount of 60% ethanol solution for ultrasonic extraction for 30 minutes, extract twice, the extraction frequency is 40KHz, concentrate the extract under reduced pressure to recover ethanol, adjust the pH of the concentrated solution to 3 with hydrochloric acid, filter, add The polyamide resin column is adsorbed. After the adsorption is completed, the impurities are first washed with water, and then eluted with 6 times the amount of 50% ethanol solution. Antimony oxide ceramic membrane filtration, the filtrate is separated by a hollow fiber membrane with a molecular weight cut-off of 1000, and the permeate is obtained, concentrated and dried, and the membrane separation component is obtained; chloroform, methanol, and water are mixed in a volume ratio of 3:10:4, After sufficient stratification, remove the lower phase and fill the high-speed countercurrent chromatographic column, turn on the host, a...

Embodiment 2

[0021] Take 1kg of the whole herb of Rhizoma chinensis, crush it, add 6 times the amount of 60% ethanol solution for ultrasonic extraction for 30 minutes, extract twice, the extraction frequency is 60KHz, concentrate the extract under reduced pressure to recover ethanol, adjust the pH of the concentrated solution to 4 with hydrochloric acid, filter, add The polyamide resin column is adsorbed. After the adsorption is completed, the impurities are first washed with water, and then eluted with 4 times the amount of 50% ethanol solution. Antimony oxide ceramic membrane is filtered, and the filtrate is separated by a hollow fiber membrane with a molecular weight cut-off of 1000 to obtain the permeate, which is concentrated and dried to obtain the membrane separation component; chloroform, methanol, and water are mixed in a volume ratio of 5:10:2, After sufficient stratification, remove the lower phase and fill the high-speed countercurrent chromatographic column, turn on the host, a...

Embodiment 3

[0023] Take 1kg of the whole herb of Rhizoma chinensis, crush it, add 12 times the amount of 60% ethanol solution for ultrasonic extraction for 60 minutes, extract once, the extraction frequency is 60KHz, concentrate the extract under reduced pressure to recover ethanol, adjust the pH of the concentrated solution to 3 with hydrochloric acid, filter, add The polyamide resin column is adsorbed. After the adsorption is completed, the impurities are first washed with water, and then eluted with 5 times the amount of 50% ethanol solution. Antimony oxide ceramic membrane filtration, and the filtrate is separated by a hollow fiber membrane with a molecular weight cut-off of 1000 to obtain the permeate, which is concentrated and dried to obtain the membrane separation component; chloroform, methanol, and water are mixed in a volume ratio of 3:5:2, After sufficient stratification, remove the lower phase and fill the high-speed countercurrent chromatographic column, turn on the host, adj...

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Abstract

The invention discloses a method for purifying Rhododendron, which is characterized in that: the round leaves of Rhizoma chinensis are used as raw materials, crushed, and 55-70% ethanol is added for ultrasonic extraction 1-3 times, the extract is concentrated under reduced pressure, and the concentrated solution is adjusted to pH 3- 4. Filtrate, add polyamide resin to the filtrate for adsorption, elute with ethanol water, concentrate the collected solution to recover ethanol, filter the concentrated solution through a high-speed centrifuge, and separate the filtrate through a membrane separator. The obtained components are separated by high-speed countercurrent chromatography and detected by an ultraviolet detector , collect the target components, and dry under reduced pressure to obtain Rhodorin. The method of the invention has the advantages of simple operation, short production period, large preparation amount and high efficiency, and is suitable for the preparation of high-purity rhodium.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry, and in particular relates to a method for purifying rhodol. Background technique [0002] Rhododendron (potengriffioside A) is a flavonoid glycoside compound with a melting point of 257-258°C and molecular formula C 27 h 30 o 14 , the structural formula: [0003] [0004] Rhododendron is derived from the dried leaves of Saccharomyces japonicus. It has anti-tumor and anti-hypertensive effects, and can be used as a xanthine oxidase inhibitor and antioxidant. [0005] The existing production processes of flavonoid glycosides mainly include solvent extraction, supercritical extraction and resin method. Among them, the cost of solvent extraction is high, the equipment investment of supercritical extraction is large, and the current degree of industrialization is low. The production of domestic flavonoid extracts basically adopts the resin adsorption method. [0006] The ultrafiltrati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
Inventor 刘东锋万冬梅
Owner NANJING ZELANG MEDICAL TECH
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