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Method for continuously extracting and separating volatile oil and flavonoid from pummelo pee leaves

A technology for separating volatilization and flavonoids, which is applied in the field of natural medicinal chemistry, can solve the problems of complicated process, complicated purification process, and low extraction efficiency, and achieve the effect of low energy consumption, less solvent consumption, and simple process

Inactive Publication Date: 2018-05-04
GUANGDONG UNIV OF PETROCHEMICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing methods for extracting two types of volatile oils and flavonoid target components with different polarities from the same material adopt separate extraction methods, resulting in complex processes, low extraction efficiency, many extracted impurities, and complicated subsequent purification processes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The invention provides a method for continuously extracting and separating volatile oil and flavonoids from the red leaves of oranges, comprising the following steps:

[0025] (1) Collect the leaves of Huajuhong, wash them, dry them in the sun or in an oven at 50°C to constant weight, crush them through a 40-mesh sieve, and obtain leaf powder for later use;

[0026] (2) Select a glass chromatography column with a diameter of 2.0cm;

[0027] (3) Put 30 g of Huaju Hongye powder on the column with petroleum ether ethanol mixture and soak in the column for 1 hour;

[0028] (4) Continuously add petroleum ether ethanol mixture to wash to make it permeate the material, flow from top to bottom, leach active ingredients, and collect 3 times the volume of eluent;

[0029] (5) Switch to 50% ethanol solution and repeat step (4), and collect 5 times the volume of the eluate;

[0030] (6) Combine the eluents from step (4) and step (5), mix well, extract and separate layers, the upp...

Embodiment 2

[0038] The invention provides a method for continuously extracting and separating volatile oil and flavonoids from the red leaves of oranges, comprising the following steps:

[0039] (1) Collect and wash the red leaves of tangerine, dry in the sun or in an oven at 60°C to constant weight, crush through a 60-mesh sieve to obtain leaf powder, and set aside;

[0040] (2) Select a glass chromatography column with a diameter of 3.0 cm;

[0041] (3) Put 50 g of Huaju Hongye powder on the column with petroleum ether ethanol mixture and soak in the column for 2 hours;

[0042] (4) Continuously add petroleum ether ethanol mixture to penetrate the material, flow from top to bottom, leach active ingredients, and collect 5 times the volume of eluent;

[0043] (5) Repeat step (4) with a volume fraction of 70% ethanol solution, and collect 7 times the volume of the eluate;

[0044] (6) Combine the eluents from step (4) and step (5), mix well, extract and separate layers, the upper layer c...

Embodiment 3

[0052] The invention provides a method for continuously extracting and separating volatile oil and flavonoids from the red leaves of oranges, comprising the following steps:

[0053] (1) Collect and wash the red leaves of tangerine, dry in the sun or in an oven at 55°C until constant weight, crush through a 50-mesh sieve to obtain leaf powder, and set aside;

[0054] (2) Select a glass chromatography column with a diameter of 2.5 cm;

[0055] (3) Put 40 g of Huaju Hongye powder on the column with petroleum ether ethanol mixture and soak in the column for 1.5 hours;

[0056] (4) Continuously add petroleum ether ethanol mixture to penetrate the material, flow from top to bottom, leach active ingredients, and collect 4 times the volume of eluent;

[0057] (5) Switch to 60% ethanol solution and repeat step (4), and collect 6 times the volume of the eluate;

[0058](6) Combine the eluents from step (4) and step (5), mix well, extract and separate layers, the upper layer contains ...

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Abstract

The invention provides a method for extracting and separating volatile oil and flavonoid from pummelo pee leaves. The method comprises the following steps: crushing and sieving the pummelo pee leaves,so as to obtain powder, and uniformly filling a powder wet sample into a glass chromatographic column; firstly extracting volatile oil with low polarity with a petroleum ether and ethanol mixed solvent with low polarity according to a like dissolves like principle, then extracting flavonoid with solvent ethanol aqueous solution with medium polarity, combining extracting solution, leaving the solution to stand for layering, and ensuring that an upper layer contains the volatile oil and the lower layer contains two types of flavone which are naringin and rhoifolin. According to the method, twomain types of substance are continuously extracted, and target products are respectively dissolved in two liquid phases because petroleum ether is automatically layered in a condition of the existenceof water, so that the purpose of separation is achieved. The method is simple in technical process, small in amount of used solvent and high in extraction efficiency, and can sufficiently use leavesusually used as waste to produce a large amount of volatile oil and flavonoid.

Description

technical field [0001] The invention relates to the field of natural medicinal chemistry, in particular to a method for continuously extracting and separating volatile oil and flavonoids from the red leaves of oranges. Background technique [0002] The original plant of Huajuhong is Huazhou pomelo, a Rutaceae plant. The fruit is a precious traditional Chinese medicine for reducing phlegm and relieving cough. All Rutaceae plants contain volatile oil, and the leaves have transparent oil glands. The content of volatile oil is more abundant than that of its fruit. The volatile oil is widely used in spices and medicine. The traditional method of extracting volatile oil is steam distillation; Contains flavonoids such as Rhodorin and Naringin, which have the effects of scavenging free radicals, anti-oxidation, anti-inflammatory, reducing phlegm and relieving cough, etc. Among them, Rhodoglutin is an inhibitor of xanthine oxidase, which can strongly inhibit the formation of uric aci...

Claims

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Application Information

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IPC IPC(8): C11B9/02A61K36/752A61K127/00
CPCA61K36/752A61K2236/333A61K2236/35A61K2236/55C11B9/022C11B9/025
Inventor 韩寒冰刘杰凤尹爱国李颖周英彪
Owner GUANGDONG UNIV OF PETROCHEMICAL TECH
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