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Total flavone of citrus grandis osbeckvar tomentosa hort peridium, and its extracting method and use

A technology of tangerine red and total flavonoids, which is applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, pill delivery, etc., can solve the problems of low yield, cumbersome, unsuitable for industrial production, etc.

Inactive Publication Date: 2005-10-05
HUAZHOU GREEN LIFE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, Jia Dongying and others have reported the extraction process of the total flavonoids of tangerine red, but it is cumbersome, not suitable for industrial production, and the cost is high, and the yield is low; the detailed extraction process of the total flavonoids of tangerine red has not been reported yet.
In addition, due to the similar structure of naringin and rhotropin, it is difficult to separate them, and there is no research report on the separation of the two by common solvent methods.

Method used

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  • Total flavone of citrus grandis osbeckvar tomentosa hort peridium, and its extracting method and use
  • Total flavone of citrus grandis osbeckvar tomentosa hort peridium, and its extracting method and use
  • Total flavone of citrus grandis osbeckvar tomentosa hort peridium, and its extracting method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take 500g of tangerine medicinal material, add 12500ml of water, soak for 0.5 hours, boil for 2 hours, filter, boil the dregs with 10000 g of water for 1.5 hours, filter, combine the filtrates, concentrate under reduced pressure to 500ml, slowly add ethanol, add as you go Stir to make the alcohol content reach 80%, filter, and wash the filter residue with an appropriate amount of 80% ethanol several times, merge into the filtrate, recover the ethanol, concentrate to 2g / ml, add an appropriate amount of distilled water to make the concentration 0.4g / ml, 5mol / ml Adjust the pH value to 1.5 with L hydrochloric acid, refrigerate, take out after 72 hours, filter, wash the precipitate with an appropriate amount of distilled water, dry at 60°C, crush, and sieve to obtain the total flavonoids of Tangerine. The content of total flavonoids should not be low in terms of naringin at 80%.

Embodiment 2

[0023] Example 2: Take 10.0 g of total flavonoids powder of tangerine red, add 35 ml of 70% methanol, and reflux in a water bath at 80 ° C. After it is completely dissolved, filter it while it is hot, and transfer the filtrate to a 50 ml Erlenmeyer flask with a stopper, and seal it. Stand at room temperature for 48 hours, separate the coarse crystals, and recrystallize the coarse crystals with 70% methanol for 1 to 2 times to obtain rhubaricin crystals (purity reaches 98.53%). Separation of the methanol solution (mother liquor) after rhotropin was recovered, methanol was placed at room temperature for 48 hours, the precipitate was separated, and acetone recrystallized 1-2 times to obtain naringin crystals (the purity of which reached 96.85%)

Embodiment 3

[0024] Example 3: The effect of total flavonoids of tangerine red on the secretion of phenol red in the trachea of ​​mice

[0025] 44 NIH mice were randomly divided into 4 groups, i.e. the high, medium and low dose groups of the total flavonoids of tangerine red (respectively; and the normal saline group). Administration was administered by intragastric administration at 20ml / kg, once a day, for four consecutive days, at the last 30 minutes after administration, intraperitoneally inject 5g / dl phenol red 0.1ml / 10g, kill the animal after 30 minutes, separate the tracheal segment, put it into a test tube filled with normal saline, add 1mol / L sodium hydroxide 0.1ml, and use a 721 type spectrometer Photometer, detection wavelength is 546nm, measures OD value, and calculates phenol red content by phenol red standard curve, q test compares the significance of difference between administration group and blank control group.The results are shown in Table 2

[0026] group

D...

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Abstract

The present invention mainly relates to a preparation method of Mao pomelo peel total flavone, Mao pomelo peel total flavone dispersing tablet, naringoside and rhoifolin and their application. The pomelo peel is a high-grade Chinese medicinal material for relieving cough and resolving phlegm. The flavone component in the Mao pomelo peel is its main effective component, in the Mao powder peel total flavone the naringoside and rhoifolin contents are higher, and are above 85% of the total flavone. Said invention provides a solvent method to make extraction, and makes the extract undergo the processes of concentration, precipitation and recrystallization so as to obtain the above-mentioned products.

Description

technical field [0001] The invention belongs to industries such as medicine and sanitation, food chemical industry and the like. The main purpose is to provide a total flavonoids of tangerine and its simple and economical extraction and separation method and application. Background technique [0002] Huajuhong is the immature or nearly ripe dry outer peel of Citrus grandis 'tomentosa' or Citrus grandis (L.) Osbeck of the Rutaceae plant. The former is commonly called "Maojuhong", and the latter is commonly called "Guangjuhong", which has the functions of dispelling cold, drying dampness, benefiting qi, and eliminating phlegm. It is mainly used for diseases such as wind-cold cough, itchy throat with excessive phlegm, food accumulation and alcohol, vomiting and nausea. Among them, Maojuhong is produced in Huazhou, Guangdong, and is an authentic medicinal material for relieving cough and reducing phlegm. [0003] The flavonoids in Maojuhong have anti-inflammatory, analgesic, ...

Claims

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Application Information

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IPC IPC(8): A61K9/20A61P29/00
Inventor 林励
Owner HUAZHOU GREEN LIFE
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