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Method for quickly screening stichopus japonicus immune enhancers with high throughput

An immune enhancer, high-throughput technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of long cycle, high cost and high risk of sea cucumber immune enhancer

Inactive Publication Date: 2009-11-25
OCEAN UNIV OF CHINA
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Problems solved by technology

However, there are many kinds of immune enhancers used in aquaculture, and the screening of japonicus japonicus immune enhancers through breeding tests takes a long time, costs a lot, and is risky, and may also be affected by the aquaculture environment (such as water temperature, water quality), japonicus seed, etc. Impact

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  • Method for quickly screening stichopus japonicus immune enhancers with high throughput
  • Method for quickly screening stichopus japonicus immune enhancers with high throughput
  • Method for quickly screening stichopus japonicus immune enhancers with high throughput

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Embodiment Construction

[0017] The method of the present invention for high-throughput and rapid screening of immune enhancers of sea cucumbers is characterized by first determining that the immune enhancers to be screened are β-glucan, peptidoglycan, chitosan, mannose oligosaccharides, and CpG-oligodeoxynucleosides. Acid, soy peptide, lactoferrin, levamisole, vitamin C, vitamin E, and then primary culture of the body cavity cells of sea cucumbers. The cultured body cavity cells of sea cucumbers were used to detect the four sensitive immune indexes of the above-mentioned immune enhancers to sea cucumbers. : The effect of phagocytic rate, superoxide anion content, superoxide dismutase activity and nitric oxide synthase activity. The screening results are determined according to the effect of immune enhancers on these four indicators. In the specific implementation, the surface of the sea cucumber is disinfected for a short time, and then the sea cucumber is dissected to obtain the body cavity fluid, which...

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Abstract

The invention discloses a method for quickly screening stichopus japonicus immune enhancers with high throughput, which is characterized by comprising the following steps: firstly determining beta-glucan, peptidoglycan, chitosan, mannan-oligosaccharide, CpG-oligodeoxynucleotide, soy peptide, lactoferrin, levamisole, vitamin C and vitamin E as the screened immune enhancers, then carrying out primary culture for stichopus japonicus coelom cells, detecting four sensitive immune indexes of the immune enhancers on stichopus japonicus by the cultured stichopus japonicus coelom cells, namely effects of phagotrophy rate, superoxide anion content, superoxide dismutase activity and nitric oxide synthase activity, and determining a screening result for the effects of the four indexes according to the immune enhancers. The method can screen the immune enhancers on large scale and in short time, and simultaneously can research the action mechanisms of the various immune enhancers and lay a foundation for the deep study of the immune enhancers.

Description

Technical field [0001] The invention relates to a method for high-throughput and rapid screening of sea cucumber immune enhancers. The method can be applied to high-throughput and large-scale screening of sea cucumber immune enhancers. Background technique [0002] Sea cucumber (Apostichopus japonicas) has high edible and medicinal value. It is a traditional Chinese precious seafood and has been hailed as one of the "eight treasures of seafood" since ancient times. In recent years, northern China has vigorously developed sea cucumber aquaculture, and sea cucumber has become one of the most valuable species in the marine aquaculture industry. However, the disease problems of sea cucumbers caused by large-scale intensive farming and irregular operations have become increasingly prominent, and there have been many obvious diseases and large-scale deaths, which have seriously affected the sustainable development of the industry. [0003] Immune enhancer refers to a class of substance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02
Inventor 谷珉麦康森张文兵马洪明许乐乐王小洁艾庆辉徐玮白楠陈效儒
Owner OCEAN UNIV OF CHINA
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