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Compound adjuvant for tuberculosis subunit vaccine, tuberculosis subunit vaccine from same and preparation method and application thereof

A subunit vaccine, tuberculosis technology, applied in medical preparations containing active ingredients, pharmaceutical formulations, bacterial antigen components, etc., can solve the problems of difficulty in mass promotion, high price of TDM, and achieve the effect of enhancing immunogenicity

Inactive Publication Date: 2013-07-17
LANZHOU UNIVERSITY
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Problems solved by technology

It has been confirmed that MH has the function of cationic liposome dimethyl hexadecyl ammonium (dimo-thylidioctyl ammonium bromide, DDA) and trehalose dimycolate (Trehalose 6,6-dimycolate, TDM) complex adjuvant Strong immunogenicity and high protection efficiency, but TDM is expensive and difficult to promote in large quantities

Method used

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  • Compound adjuvant for tuberculosis subunit vaccine, tuberculosis subunit vaccine from same and preparation method and application thereof
  • Compound adjuvant for tuberculosis subunit vaccine, tuberculosis subunit vaccine from same and preparation method and application thereof
  • Compound adjuvant for tuberculosis subunit vaccine, tuberculosis subunit vaccine from same and preparation method and application thereof

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Embodiment 1

[0029] 1. Experimental materials:

[0030] The fusion protein MH was prepared by Lanzhou University, and the preparation method is detailed in the patent application "Construction, Expression, Purification and Application of Mycobacterium Tuberculosis Fusion Protein Mtb10.4-Hsp16.3" (application number is CN201010613320.6, publication number is CN102154324A); CpG-ODN was synthesized by Shanghai Sangong;

[0031] Polyinosinic: polycytidylic acid, PolyI: C) was purchased from Sigma-Aldrich Company, product number CAS42424-50-0;

[0032] Dimethyl hexadecyl ammonium (DDA) was purchased from Sigma-Aldrich Company, product number CAS3700-67-2;

[0033] Bacillus Calmette-Guerin (BCG, Danish strain), donated by Lanzhou Institute of Biological Products;

[0034] PBS was prepared by Lanzhou University with conventional preparation methods.

[0035] 2. Experimental animals: C57BL / 6 mice.

[0036] 3. Grouping of experimental animals (8 groups in total):

[0037] A. PBS (200μl each ...

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Abstract

The present invention discloses a compound adjuvant for tuberculosis subunit vaccine, containing cationic liposome dimo-thylidioctyl ammonium bromide (DDA), TLR9 ligand CpG oligodeoxynucleotides 2395 and TLR3 ligand PolyICLC, and also provides the tuberculosis subunit vaccine and preparation method and application thereof. The beneficial effect of the invention is that the compound adjuvant for tuberculosis subunit vaccine provided by the present invention enhances the immunogenicity of the fusion protein of Mycobacterium tuberculosis, contributing to the induction of the tuberculosis fusion protein to Th1-type anti-tuberculosis immune response.

Description

technical field [0001] The invention relates to the field of biological preparations, in particular to a tuberculosis subunit vaccine compound adjuvant, a tuberculosis subunit vaccine constructed using it, a preparation method and application. Background technique [0002] Preventive tuberculosis vaccine—BCG (Bacille Calmette Guérin, BCG) has unstable protection for adults, so it is imminent to develop new tuberculosis vaccines, especially protein vaccines with low cost, high safety, and not affected by environmental mycobacteria. extensive attention. However, the immunogenicity of protein vaccines is weaker than that of attenuated vaccines, so appropriate adjuvants must be selected to enhance the immune effect of vaccines. [0003] For more than 70 years, aluminum adjuvant has been widely used in the world as the only registered adjuvant. Another adjuvant, MF59, has been registered in some countries as an adjuvant for influenza and hepatitis A vaccines. However, the abov...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K39/04A61K9/107A61P31/06
Inventor 李青祝秉东刘宝元牛红霞苏娟唐克峰
Owner LANZHOU UNIVERSITY
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