75 results about "Thermolabile" patented technology

A method of treating a coal combustion flue gas, which includes injecting a molecular halogen or thermolabile molecular halogen precursor, such as calcium hypochlorite, able to decompose to form molecular halogen at flue gas temperature. The molecular halogen converts elemental mercury to mercuric halide, which is adsorbable by alkaline solids such as subbituminous or lignite coal ash, alkali fused bituminous coal ash, and dry flue gas desulphurization solids, capturable in whole or part by electrostatic precipitators (ESPs), baghouses (BHs), and fabric filters (FFs), with or without subsequent adsorption by a liquid such as a flue gas desulphurization scrubbing liquor.

A method of treating a coal combustion flue gas, which includes injecting a molecular halogen or thermolabile molecular halogen precursor able to decompose to form molecular halogen at flue gas temperature. The molecular halogen coverts elemental mercury to mercuric halide adsorbable by alkaline solids such as subbituminous or lignite coal ash, alkali fused bituminous coal ash capturable in whole or part by electrostatic precipitators (ESPs), baghouses (BHs), fabric filters (FFs), dry flue gas desulphurization solids, with or without subsequent adsorption by a liquid such as a flue gas desulphurization scrubbing liquor.

A mold is disclosed for forming a freezable and thawable bag of uniform thickness, especially useful for cryopreservation of thermolabile substances. The mold features one or more recessed planar surfaces, including radiused peripheral walls about the recesses, a planar top surface, and means for conforming a blank sheet to the mold. Two mold halves combine to form a completed bag. The mold halves may be complimentary.

A bag, method of manufacture and process are disclosed for the cryopreservation of thermolabile substances. The bag is characterized as having substantially uniform thickness throughout its length and height. The bag features a radiused peripheral edge wall for stress relief and to provide the constant cross-section. A peripheral flashing circumscribes the radiused edge wall and provides a suitable purchase area for sealing so that the thus formed bag is less susceptible to fracture particularly when exposed to cryogenic temperatures. The uniform thickness of the bag promulgates uniform heat transfer to and from the contents of the bag in relation to any surrounding medium at a different temperature. The bag affords more space for efficient storage and reduces heat invasion into the contents of the bag when a plurality of bags are placed with their larger planar surfaces in contact with each other.

A bag, method of manufacture and process are disclosed for the cryopreservation of thermolabile substances. The bag is characterized as having substantially uniform thickness throughout its length and height. The bag features a radiused peripheral edge wall for stress relief and to provide the constant cross-section. A peripheral flashing circumscribes the radiused edge wall and provides a suitable purchase area for sealing so that the thus formed bag is less susceptible to fracture particularly when exposed to cryogenic temperatures. The uniform thickness of the bag promulgates uniform heat transfer to and from the contents of the bag in relation to any surrounding medium at a different temperature. The bag affords more space for efficient storage and reduces heat invasion into the contents of the bag when a plurality of bags are placed, with their larger planar surfaces, in contact with each other.

A system and method for controlled rate freezing and storage of thermolabile substances. The system includes a storage unit for receiving product stored within a bag and an overlying protective canister associated with a robotic arm and reading device which places the canister in the preserving environment. A control system, driven by a computer monitors the ingress, egress and storage location and particularized profiles of the articles being placed in storage.

Disclosed herein are novel targeted drug delivery and controlled release methods and compositions where optically absorbing nanoparticles, such as nanoshells, are functionalized on their surfaces with thermolabile molecules that bind the drug molecules to be delivered. The linkage between the thermolabile moiety on the nanoparticles and the drug is deliberately designed or selected to be temperature sensitive, so that upon illumination of the nanoparticle at a wavelength of light, the drug molecules on the nanoparticles will be released. Targeting molecules, such as antibodies, aptamers or other molecules like folic acid, can be concurrently bound to the nanoparticle surface to deliver the nanoparticle to specifically targeted cells or tissues prior to the photothermally induced drug release. In this way the nanoparticles can be advantageously concentrated on the target prior to illumination, which makes the disclosed compositions both a targeted delivery and a controllable drug release vehicle.

The invention provides a multiplex quantitative PCR (polymerase chain reaction detection kit for vibrio parahaemolyticus toxin gene and a detection method. The kit mainly comprises specific primers, probes and PCR reaction reagent, wherein the specific primers and the probes consist of specific primers and probes of vibrio parahaemolyticus thermostable direct hemolysin gene (tdh), thermolabile hemomysin gene (tlh), toxin expression regulating protein gene (toxR) and thermostable related hemolysin gene (trh). The invention provides the quick, sensitive and specific multiplex fluorescent quantitative PCR detection kit and the detection method aiming at the vibrio parahaemolyticus toxin gene, and provides basis for controlling food poisoning caused by the vibrio parahaemolyticus in time and early diagnosis of the food poisoning caused by the vibrio parahaemolyticus.

The present invention provides parenteral adjuvants comprising detoxified mutants of bacterial ADP-ribosylating toxins, particularly those from pertussis (PT), cholera (CT), and heat-labile E. coli (LT).

The invention relates to compositions, methods, and kits for hot start polynucleotide synthesis, including extension of primed polynucleotide templates and polymerase chain reaction (PCR). Hot start is provided by a thermally inactivated blocking polymerase protein that binds primed polynucleotide templates and prevents their access to a thermostable nucleic acid polymerase. High temperatures employed in the synthesis reaction cause the blocking polymerase to denature, thereby permitting the action of a thermostable processive polymerase. Compositions of the invention include a specific blocking polymerase protein which is a mutant of the Klenow fragment of E. coli DNA polymerase. The mutant is essentially devoid of polymerase activity, processivity, and 3′ to 5′ exonuclease activity. Use of the thermally inactivated blocking polymerase together with a thermostable polymerase reduces non-specific priming and accumulation of unwanted amplification products, increasing the specificity and sensitivity of the synthesis reaction.

The invention relates to a steam sterilizer and a sterilization process, and in particular relates to a negative-pressure and low-temperature steam sterilizer and the sterilization process. The steam sterilizer comprises a sandwich layer and an internal chamber, wherein the internal chamber consists of a space enclosed by a front door, a back door and the sandwich layer; a steam channel is formed in the sandwich layer; the sandwich layer is connected with a sandwich layer steam inlet system through a pipeline; the internal chamber is connected with an internal chamber emptying system through a pipeline; the sandwich layer is communicated with the internal chamber through a pipeline, and a first pneumatic control valve is arranged on the pipeline; the internal chamber is connected with an internal chamber vacuumizing drainage system through a pipeline; and the sandwich layer is connected with a sandwich layer vacuumizing drainage system through a pipeline. The steam sterilization process comprises: (1) vacuumizing; (2) sandwich layer steam inlet; (3) heating; (4) sterilization. The low-temperature steam sterilization of a thermolabile article is realized; by virtue of the internal chamber vacuumizing drainage system, condensed water formed in the sterilization process can be effectively drained, and the steam quality and temperature uniformity of the internal chamber are ensured.

The invention relates to a microencapsulated embedded probiotic and a processing method thereof. The microencapsulated probiotic mainly comprises a wall material and a core material, wherein the wall material embeds the core material to form a microcapsule and the core material is a probiotic. The processing method includes dissolving the probiotic into water, adding the wall material, performing high-pressure homogenization to allow the probiotic and the wall material to form a fully uniform emulsion, and drying the emulsion until the water content is lower than 5% to form the microcapsule. The probiotic is a probiotic easy in moisture absorption and / or non-resistant to heat. Compared with un-embedded probiotics, the microencapsulated embedded probiotic is obviously improved in moisture absorptivity and heat resistance. The probiotic is convenient in storage, transportation and using. In particular, after the heat resistance is improved, the probiotic can be provided to human bodies through food cooking.

The present invention provides a CpG oligonucleotide prodrug that includes a thermolabile substituent on at least one nucleotide thereof. The present invention also provides compositions that include a carrier and a therapeutically effective amount of at least one CpG oligonucleotide prodrug. The present invention further provides therapeutic methods of using such thermolabile CpG oligonucleotide prodrugs and compositions thereof. The present invention further provides a method of inhibiting tetrad formation in a CpG oligonucleotide by functionalizing the CpG oligonucleotide with one or more thermolabile substituents.

This disclosure relates to a method and a system of producing nanoparticles and nanoparticle matrices of thermolabile, biocompatible matrix materials, like lipids and biopolymers with controlled properties. A prototype pulse-heat aerosol system is described for single-step production of free, thermolabile nanoparticles with sufficient control over size, morphology and crystallinity with controlled-release properties, for possible therapeutic, cosmetic or diagnostic use. Nanoparticles of the range 50 to 500 nm are obtained and are found suitable for controlled drugs delivery.

The present disclosure is directed to compositions and methods for making a pharmaceutical composition by thermokinetic compounding, wherein the compositions include one or more thermolabile components, for example one or more active pharmaceutical ingredients (API) with one or more pharmaceutically acceptable excipients. The methods comprise thermokinetic processing of the thermolabile components into a composite by blending certain thermolabile components in a thermokinetic mixer using multiple speeds during a single, rotationally continuous operation. The composite can be further processed into pharmaceutical compositions by conventional methods known in the art, such as hot melt extrusion, melt granulation, compression molding, tablet compression, capsule filling, film-coating, or injection molding.

Provided is a hydroxyl-protected alcohol comprising a thermolabile hydroxyl-protecting group comprising a 2-pyridyl substituent and a precursor of the thermolabile hydroxyl-protected alcohol. An exemplary thermolabile hydroxyl-protected alcohol is represented by the formula Pg-O—R, wherein Pg is a protecting group of the formula: (Formula) wherein: A is a 2-pyridyl; Z is CH2 or NR1; R1, R2, R2′, R3 and R3′ are the same or different and each can be, e.g., H, alkyl, or alkyl comprising an aryl substituent; W is CO, CS, or SO; and R is the organic residue of the hydroxyl-protected alcohol. Also provided is a method of producing an alcohol, which method comprises heating the hydroxyl-protected alcohol, which optionally may be obtained from a precursor, at a temperature effective to cleave the hydroxyl-protecting group. The method can be used to produce oligonucleotides.

Process for producing a product for feeding animals comprising a first step of producing kibbles for therapeutic purpose through a first step of providing at least one thickener component in form of powder, at least one hydrophilic mineral in form of powder and at least one therapeutic substance in form of powder for curing animals, containing thermolabile and / or oxide-reducing active ingredients; a step for mixing such ingredients, aimed at homogenising such ingredients together; and a step for dry-pressing mixed powders to obtain compact agglomerates in form of kibbles, for therapeutic purpose, having lipophile characteristics and comprising a moisture percentage lower than 6%. The process also comprises a step for producing kibbles for nutritional purpose by means of extrusion starting from a mixture, a step for coating with fat, wherein the latter kibbles are coated with fat on the surface, a mixing step, wherein the kibbles for therapeutic purpose are mixed at percentages comprised between 5 and 15% to the kibbles for nutritional purpose, for uniform distribution thereinThe process further comprises a step for absorbing—by the kibbles for therapeutic purpose—part of the surface fats of the kibbles for nutritional purpose, such absorption step being at least partly performed during the mixing step.

The invention discloses a method for detecting vibrio parahemolyticus through combination of unlabelled fluorescent PCR and HRMA, which is characterized by including the following steps: firstly, a primer pair is designed according to a TLH (thermolabile hemolysin) gene of the vibrio parahemolyticus; secondly, after DNA (Deoxyribonucleic acid) samples are extracted, unlabelled fluorescent PCR amplification is performed by utilizing the designed primer pair; and thirdly, an amplification curve of a PCR amplification product and the HRMA are analyzed by application software. The purposes of the method are to overcome defects of the prior art and provide a method, which is simple, convenient and fast to operate, accurate in detection result and low in usage cost, for detecting the vibrio parahemolyticus through the combination of the unlabelled fluorescent PCR and the HRMA.

Provided is a hydroxyl-protected alcohol of the formula R—O—Pg, wherein Pg is a protecting group of the formula:wherein Y, Z, W, R1, R1a, R2, R2a, R3, R3a, R4, R4a, a, b, c, d, e and f are defined herein and R is a nucleosidyl group, an oligonucleotidyl group with 2 to about 300 nucleosides, or an oligomer with 2 to about 300 nucleosides. Also provided is a deprotection method, which includes heating the hydroxyl-protected alcohol at a temperature effective to cleave thermally the hydroxyl-protecting group therefrom.

Provided is a hydroxyl-protected alcohol comprising a thermolabile hydroxyl-protecting group comprising a 2-pyridyl substituent and a precursor of the thermolabile hydroxyl-protected alcohol. An exemplary thermolabile hydroxyl-protected alcohol is represented by the formula Pg-O—R, wherein Pg is a protecting group of the formula: (Formula) wherein: A is a 2-pyridyl; Z is CH2 or NR1; R1, R2, R2′, R3 and R3′ are the same or different and each can be, e.g., H, alkyl, or alkyl comprising an aryl substituent; W is CO, CS, or SO; and R is the organic residue of the hydroxyl-protected alcohol. Also provided is a method of producing an alcohol, which method comprises heating the hydroxyl-protected alcohol, which optionally may be obtained from a precursor, at a temperature effective to cleave the hydroxyl-protecting group. The method can be used to produce oligonucleotides.

A modular construction micro-reactor with parallel microchannels on fluid guide plates for chemical reactions, but not for evaporation purposes is known. A modular construction falling film evaporator is disclosed, comprising a stack of alternate gap-like evaporation chambers and sheet-like evaporator modules with an assembly of parallel microchannels, whereby the evaporation chambers are open above and / or below across the whole width of the module and the stack is arranged in a container. The falling film evaporator is used in a method for obtaining a gaseous phase from a liquid medium on a technical scale and is suitable for the concentration of thermolabile solutions and rapidly adjustable production of a gas stream.

The present disclosure is directed to compositions and methods for making a pharmaceutical composition by thermokinetic compounding, wherein the compositions include one or more thermolabile components, for example one or more active pharmaceutical ingredients (API) with one or more pharmaceutically acceptable excipients. The methods comprise thermokinetic processing of the thermolabile components into a composite by blending certain thermolabile components in a thermokinetic mixer using multiple speeds during a single, rotationally continuous operation. The composite can be further processed into pharmaceutical compositions by conventional methods known in the art, such as hot melt extrusion, melt granulation, compression molding, tablet compression, capsule filling, film-coating, or injection molding.

The invention provides a light-cured addition type organopolysiloxane composition, which comprises the following ingredients (by weight): 80-90 parts of alkenyl netted or chain polysiloxane, 8-19.5 parts of polysiloxane containing a silicon-hydrogen bond, 2-60 ppm parts of an UV photo-initiation catalyst, 0.5-2 parts of a photo-initiation aid, and 2-50 ppm parts of a polymerization inhibitor. According to the invention, a siloxane material is cured by a room-temperature photocuring mode. In comparison with the prior art, long-time high-temperature heating is not required in the invention. Therefore, defects of long heating time and long total reaction time of a traditional material preparation method are eliminated. In addition, the product is applicable to a low-temperature thermolabile substrate. Thus, reaction time is saved, reaction rate is increased, and damage to a thermolabile substrate is reduced.

Disclosed is a method of manufacturing a bag (20) for the cryopreservation of thermolabile liquids. The manufacturing method is characterized by being versatile, simple and inexpensive and allows to manufacture single and multi-compartment bags without modifying the main sealing molds (31, 32). The relative size and the number of the compartments (28) can be modified without change in the molds. Only a closure sealing tool (50) must be adjusted as a function of the number of chosen compartments (28). The process is compatible with standard high frequency sealing processes. This method provides bags (20) with uniformly thick walls and with a predetermined bag volume.

The invention provides a serum preparation method and a serum preparation apparatus with which a large amount of serum capable of exhibiting a high culture efficiency regardless of freshness of blood to be used can be obtained at once. In a serum preparation method in which serum is prepared from blood containing at least platelets, a platelet treatment step in which a treatment of disrupting thecell membrane of the platelets in the blood is performed is provided. It is preferred that after the platelet treatment step, a deposition step in which a heat-labile protein in the blood is deposited and a removal step in which the heat-labile protein deposited by this deposition step is removed are provided.

Provided are a hydroxyl-protected alcohol of the formula R—O-Pg, wherein Pg is a protecting group of the Formulae (I), (II), wherein Y is R1, OR1, or NR1R1a; Z is O, NR2 or CR2R2a; W is CO or SO; R1, R1a, R2, R2a, R3, R3a, R4 and R4a include H, a saturated or unsaturated alkyl, an aryl, and a saturated or unsaturated alkyl comprising an aryl; a, b, c, d, e and f include H, a halogen, a saturated or unsaturated alkyl, a hydroxyl, an alkoxy, an aryloxy, an aralkoxy, a cyano, a nitro, a sulfhydryl, an alkyl or aryl sulfoxy, are alkyl or aryl sulfonyl, a keto, a thioketo, an ester, an amide, an amino, an alkylamino or a dialkylamino; and R represents the organic residue of the hydroxyl-protected alcohol; a hydroxyl-protected alcohol which includes a thermally cleavable 2-amidoethoxycarbonyl hydroxyl-protecting group; and a deprotection method, which includes heating the hydroxyl-protected alcohol at a temperature effective to cleave thermally the hydroxyl-protecting group therefrom.

Chewing gum are obtained by entrapping a liquid within a mass of gum, blast freezing the gum and entrapped liquid, and increasing the temperature to just below the melting point of the liquid, causing the liquid crystals to anneal. By manipulating the size and / or morphology of the frozen liquid crystals, one can thereby manipulate the internal structure of the gum. Optionally, the liquid can contain a drug in solution. If the solvent is then removed, the drug is left in the interstices of the gum. The rate of release of the drug from the gum is thus determined by the distribution of the drug within the gum arising from the annealing step. This method of manufacturing a chewing gum avoids the use of elevated temperatures, and is particularly suitable for entrapping thermolabile substances.

The invention relates to a fly maggot heat-resistant protein extract and a preparation method thereof. The fly maggot heat-resistant protein extract prepared by the steps of rinsing and sterilizing of fresh fly maggots, extraction of crude protein, removal of heat-labile protein, desalination, freeze-drying and the like has remarkable anti-diarrhea effect. According to the preparation method, loss of effective substance of fly maggots during conventional processes such as sun-drying is reduced greatly since fresh fly maggots are used as medicine directly; extracting solution contains essential substances such as buffer salt and antioxidant which can effectively prevent inactivation of the effective substances; fly maggots are homogenized completely by the extracting solution in rational proportion so that cells of fly maggots are ruptured, the effective substances in bodies of the fly maggots are released completely, and dissolution of the effective substances of the fly maggots is improved; and anti-diarrhea effect of the fly maggot heat-resistant protein extract is improved further by procedures of centrifugal degreasing, impurity removal, removal of heat-labile components by water bath and the like after homogenization.