The invention discloses a pungent and spicy barbecue sauce. The pungent and spicy barbecue sauce is not added with any spice essence and preservative, is heavy in barbecue flavor, obvious in main flavor, long in quality guarantee period and strong in tenderizing effect. Especially, due to the scientific compounding of the barbecue sauce, the taste, flavor and functional characteristics (bacterial inhibition, oxidation resistance and pharmaclogical property) of the barbecue food are improved, and comprehensive flavor substances of food materials in a barbecue state are collected, so that the barbecue sauce has heavy and attractive distinctive barbecue flavor, and the problem that food materials have no barbecue flavor before roasting is solved; meanwhile, by virtue of scientific compounding of a base sauce and compound enzyme, the cholesterol contents of food materials and auxiliary materials are reduced, and a new functional substance cholest-4olefine-3ketone is generated, so that the possibility that cholesterol generates carcinogens during high-temperature roasting or combustion is completely eradicated, the food safety is improved, the prepared barbecue food is fresh and tender in texture, fresh and delicious in taste, heavy in barbecue flavor, low in cholesterol content and strong in food safety, and therefore, a new shortcut for preparing barbecue foodstuff with distinctive barbecue flavor is explored.