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Complex immunity adjuvant, and preparation method and application thereof

A compound immune adjuvant and vaccine technology, applied in the field of medicine and biology, to achieve the effect of improving the level of immune response, concentrating the particle size distribution, and improving the immune effect

Active Publication Date: 2017-06-06
北京康宝利华生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, these adjuvants are only partially used in the research of commercial attenuated vaccines, inactivated vaccines, DNA vaccines, synthetic peptides and recombinant peptide vaccines, but only a small number of adjuvants can improve the protective effect of vaccines. PRRSV There is still a lot of room for development in vaccine immune adjuvants

Method used

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  • Complex immunity adjuvant, and preparation method and application thereof
  • Complex immunity adjuvant, and preparation method and application thereof
  • Complex immunity adjuvant, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: (1) Preparation of Houttuynia cordata polysaccharide

[0035] Houttuynia cordata polysaccharide is prepared from dried Houttuynia cordata by the following process: raw material of dried houttuynia cordata → pulverizer crushing → hot water extraction → centrifugation, suction filtration → filtrate → vacuum concentration to 1 / of the original volume 8 Alcohol precipitation overnight → centrifugal separation, washing → vacuum drying → Houttuynia cordata polysaccharide, the specific process includes the following steps:

[0036] Step 1, taking dried Houttuynia cordata as raw material, using a pulverizer to pulverize it to 120 mesh to obtain Houttuynia cordata powder;

[0037] Step 2, mix Houttuynia cordata powder with warm water at 68°C in a ratio of 20:1 for hot water extraction, keep the extraction temperature at 68°C, stir and extract at a speed of 40rpm for 2.5 hours, and obtain Houttuynia cordata hot water immersion Extract;

[0038] Step 3: centrifuge the...

Embodiment 2

[0041] Embodiment 2: A compound immune adjuvant, consisting of the following components in weight percentage: Houttuynia cordata polysaccharide 2.5%, alanine 0.2%, ginkgo leaf flavonoids 1.4%, polyethylene castor oil 7%, Span80 7 %, polyethylene glycol 3.4%, squalene 8%, soybean oil for injection 32%, water for injection 38.5%.

[0042] The preparation method of described composite immune adjuvant comprises the steps:

[0043] Step 1, each raw material is weighed according to the weight percentage ratio;

[0044] Step 2, mix the weighed Houttuynia cordata polysaccharide and alanine with water for injection, stir until it is fully solvent, and obtain the water phase;

[0045] Step 3, mixing the weighed ginkgo leaf flavonoids with squalene and soybean oil for injection, and stirring evenly to obtain an oil phase;

[0046] Step 4, mix the weighed polyethylene castor oil, Span80 and polyethylene glycol, then add the oil phase prepared in step 3, and stir for 4 minutes at a speed...

Embodiment 3

[0048] Embodiment 3: A compound immune adjuvant, consisting of the following components in weight percentage: Houttuynia cordata polysaccharide 1.5%, alanine 0.55%, ginkgo leaf flavonoids 2.2%, polyethylene castor oil 5%, Span80 8 %, polyethylene glycol 5%, squalene 6%, soybean oil for injection 34%, water for injection 37.75%.

[0049] The preparation method of described composite immune adjuvant comprises the steps:

[0050] Step 1, each raw material is weighed according to the weight percentage ratio;

[0051] Step 2, mix the weighed Houttuynia cordata polysaccharide and alanine with water for injection, stir until it is fully solvent, and obtain the water phase;

[0052] Step 3, mixing the weighed ginkgo leaf flavonoids with squalene and soybean oil for injection, and stirring evenly to obtain an oil phase;

[0053] Step 4, mix the weighed polyethylene castor oil, Span80 and polyethylene glycol, then add the oil phase prepared in step 3, and stir for 5 minutes at a speed...

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Abstract

The invention discloses a complex immunity adjuvant. The adjuvant is prepared from the following components in percentage by weight: 1.5 to 3.5 percent of horttuyia cordala thunb polysaccharide, 0.2 to 0.55 percent of alanine, 0.8 to 2.2 percent of ginkgo leaf flavonoid, 5 to 8 percent of polyethylene castor oil, 5 to 8 percent of Span 80, 2.5 to 5 percent of polyethylene glycol, 6 to 10 percent of squalene, 30 to 35 percent of soybean oil for injection and 35 to 50 percent of water for injection, and the sum of the percentage by weight of the various components is 100 percent. The complex immunity adjuvant is safe and effective, can effectively enhance the immunostimulation capacity of PRRSV vaccines, and is suitable for immunoprophylaxis of porcine reproductive and respiratory syndrome (PRRS).

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and relates to a compound vaccine adjuvant, in particular to a compound vaccine adjuvant capable of specifically enhancing the immunostimulatory ability of porcine reproductive and respiratory syndrome virus (PRRSV) inactivated vaccines. Background technique [0002] porcine reproductive and respiratory syndrome (porcine reproductive and respiratory syndrome) Porcine reproductive and respiratory syndrome, PRRS ) is caused by porcine reproductive and respiratory syndrome virus ( Porcine reproductive and respiratory syndrome virus, PRRSV ) caused by sow abortion and piglet respiratory disorders as the main features of viral infectious disease, and can cause severe immunosuppression. Since the 1990s, the virus has spread widely around the world, causing huge economic losses to the world's pig industry, becoming one of the major diseases in large-scale pig farms around the worl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/12A61P31/14A61P37/04
CPCA61K39/12A61K39/39A61K2039/5252A61K2039/5254A61K2039/55511A61K2039/55583C12N2770/10034
Inventor 陈瑞张磊张志刚
Owner 北京康宝利华生物科技有限公司
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