Immunoenhancer and application thereof

An immune enhancer and an immune enhancement technology are applied in the field of swine foot-and-mouth disease vaccine compositions to achieve good immune effects, broad application prospects, and the effects of enhancing the level of cellular immune responses.

Active Publication Date: 2018-06-05
CHINA ANIMAL HUSBANDRY IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with B cell epitopes and Th cell epitopes of FMD, there are few studies on cytotoxic T cell CTL epitopes. Some studies have confirmed that FMDV-specific CTL epitopes can induce cellular immune responses in the body. Therefore, The development of immune enhancers of CTL epitopes has important application significance for cell-mediated anti-FMDV immunity

Method used

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  • Immunoenhancer and application thereof
  • Immunoenhancer and application thereof
  • Immunoenhancer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1, Screening of CTL epitope polypeptides

[0036] It is predicted that after the polypeptide immunizes the body, the polypeptide antigen presented by MHC class I molecules can stimulate the body to produce memory T cells. Memory T cells induce the production of cytokines when the polypeptide antigen stimulates T cells again. Whether the predicted polypeptide is a CTL epitope polypeptide can be evaluated and analyzed through the detection of cytokine levels and biostatistical analysis.

[0037] 1. Prediction of peptides:

[0038] 10 predicted peptides ZM1501-ZM15010 were predicted and chemically synthesized from the website, and the sequence information is shown in the sequence list. 20mM Tris-HCl (pH 8.0) and 50mM NaCl buffer solutions were used to dissolve the predicted polypeptides respectively, and all 10 predicted polypeptides were prepared into a solution with a concentration of 1mg / ml. Each predicted polypeptide was mixed with 61 adjuvants at a volume...

Embodiment 2

[0065] Example 2, Application of CTL Epitope-containing Polypeptide Vaccine Composition

[0066] 1. Three functional CTL epitope polypeptides obtained by screening in Example 1: ZM1501, ZM1502 and ZM1503 were dissolved in 20mM Tris-HCl (pH 8.0), 50mMNaCl buffer solution. The three CTL epitope polypeptides were prepared into a solution with a concentration of 1 mg / ml. in,

[0067] ZM1501 sequence: SADPVTATV (sequence 1 in the sequence listing)

[0068] ZM1501 sequence: ALDNTTNPT (sequence 2 in the sequence listing)

[0069] ZM1501 sequence: LM QTPSHTL (sequence 3 in the sequence listing)

[0070] According to the immunization dose and immunization group, take each CTL epitope polypeptide or prepare a mixed solution and mix it with the O / Mya98 / XJ / 2010 inactivated antigen containing the immunization dose, incubate overnight at 4°C, and mix the obtained mixed solution with Montanide ISA 206 adjuvant The porcine foot-and-mouth disease vaccine composition is prepared.

[0071...

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Abstract

The invention provides an immunoenhancer, a swine foot and mouth disease vaccine composition containing the immunoenhancer and application. The immunoenhancer is prepared from three swine foot and mouth disease virus CTL epitope polypeptides, i.e., one or a random combination of two or more of a polypeptide shown as a sequence 1 in a sequence table, a polypeptide shown as a sequence 2 in the sequence table and a polypeptide shown as a sequence 3 in the sequence table. The swine foot and mouth disease vaccine composition provided by the invention is prepared from the immunoenhancer and a swinefoot and mouth disease antigen. The immunoenhancer provided by the invention can be prepared in a large batch through a solid-phase chemical synthesis method and is mixed with an inactivation antigenaccording to a certain ratio, so that the vaccine composition can be produced and prepared in a large scale; the vaccine composition can be used for inducing organisms to generate a body fluid immuneresponse level and also can be used for enhancing a cell immune response level of the organisms and obtaining a relatively good immune effect. The immunoenhancer provided by the invention is safe andreliable and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products. Specifically, the invention relates to an immune enhancer, a porcine foot-and-mouth disease vaccine composition containing the immune enhancer and its application. Background technique [0002] Foot and Mouth Disease (FMD) is an acute, febrile, highly contagious and rapid long-distance disease that occurs in pigs, cattle, sheep and other cloven-hoofed animals caused by Foot-and-Mouth Disease virus (FMDV). The spread of severe infectious diseases is known for its strong infectivity, rapid disease transmission, and serious harm. The outbreak of the disease has caused huge economic losses to the animal husbandry in the published area (Chang Huiyun, 2017). The World Organization for Animal Health (OIE) lists it as one of the multiple zoonotic infectious diseases that must be notified, and my country lists it as a first-class animal infectious disease. [0003] Foot-and-mouth d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06A61K38/08A61K39/135A61P31/14A61P37/04
CPCA61K38/08A61K39/12A61K2039/5252A61K2039/5258A61K2039/552A61K2039/572A61K2039/575A61P31/14A61P37/04C07K7/06A61K2300/00
Inventor 肖进王振豹巴利民张欣王楠董春娜张蕾赵洪涛张艳宾
Owner CHINA ANIMAL HUSBANDRY IND
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