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Ionic liquid-graphene nanocomposite, preparation method and electrochemical immunodetection method thereof

A nanocomposite and ionic liquid technology, which is applied in the field of ionic liquid-graphene nanocomposite, its preparation method and electrochemical immunoassay, can solve the problem of inability to accurately measure the content, high requirements for testing instruments, and accuracy of serological methods , low specificity and other issues

Inactive Publication Date: 2012-11-14
THE THIRD AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIV OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the immunohistochemical method has high requirements for testing instruments, and can only detect APE1 qualitatively, but cannot accurately measure the content of APE1. The accuracy and specificity of serological methods are not high.

Method used

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  • Ionic liquid-graphene nanocomposite, preparation method and electrochemical immunodetection method thereof
  • Ionic liquid-graphene nanocomposite, preparation method and electrochemical immunodetection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Preparation of ionic liquid-graphene nanocomposite

[0050] 5mg graphene was uniformly dispersed with 5mL 1wt% cetyltrimethylammonium bromide (CTAB, cetyltrimethylammonium bromide) solution, and ultrasonically treated to obtain CTAB-graphene composite; the 1mL CTAB-graphene composite was (1mg / mL) was added to 0.5mL, 5wt% polysodium 4-styrenesulfonate (PSS, Polysodium 4-styrenesulfonate) solution to prepare graphene nanosheets with negative charges on the surface; the surface was negatively charged The graphene nanosheets were ultrasonically dispersed and mixed with 0.5 mL of 1 mM ionic liquid [bmim]BF4 to prepare room temperature ionic liquid-graphene nanocomposites.

Embodiment 2

[0051] Example 2 Preparation of ionic liquid-graphene nanocomposite

[0052] 5mg of graphene was uniformly dispersed with 5mL of 1wt% dodecyltrimethylammonium chloride solution, and the CTAB-graphene composite was obtained by ultrasonic treatment; the 1mL CTAB-graphene composite (1mg / mL) was added to into 0.5mL, 5wt% sodium polyacrylate solution to prepare graphene nanosheets with negative charges on the surface; ultrasonically disperse the graphene nanosheets with negative charges on the surface, and mix them with 0.5mL of 1mM ionic liquid [bmim] BF4 was mixed to prepare room temperature ionic liquid-graphene nanocomposites.

Embodiment 3

[0053] Example 3 Preparation of ionic liquid-graphene nanocomposite

[0054] 5mg graphene was uniformly dispersed with 5mL 1wt% dodecyltrimethylammonium bromide solution, and ultrasonically treated to obtain CTAB-graphene composite; the 1mL CTAB-graphene composite (1mg / mL) was added into 0.5mL, 5wt% sodium p-methylstyrene sulfonate solution to prepare graphene nanosheets with negative charges on the surface; ultrasonically disperse the graphene nanosheets with negative charges on the surface, and mix with 0.5mL 1mM The room temperature ionic liquid-graphene nanocomposites were prepared by mixing the ionic liquid [bmim]BF4.

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Abstract

The invention relates to the field of electrochemical immunodetection, and especially relates to an ionic liquid-graphene nanocomposite, a preparation method and an electrochemical immunodetection method thereof. According to the immunodetection method, through using a double-antibody sandwich method, an apurinic / apyrimidinic endonuclease / redox factor antibody (anti-APE1) fixedly carried on the surface of an electrode carries out immunoreaction with an apurinic / apyrimidinic endonuclease / redox factor (APE1) in a sample solution, and is then combined with a room-temperature ionic liquid-graphene nanocomposite and an anti-APE1 co-coupling object marked by alkaline phosphatase (ALP) and ferrocene (Fc). Based on the electrochemical activities of the ALP-Fc-anti-APE and the room-temperature ionic liquid-graphene nanocomposite, a CV (cyclic vohammetry) catalytic current value is measured, and then the concentration of the APE1 in a detection sample is detected. The linear response range of the electrochemical immunodetection method provided by the invention is 0.1-80 pg / mL, and the lower detection limit is 0.04 pg / mL, therefore, the electrochemical immunodetection method is good in specificity and high in sensitivity.

Description

technical field [0001] The invention relates to the field of electrochemical immunodetection, in particular to an ionic liquid-graphene nanocomposite, a preparation method thereof and an electrochemical immunodetection method. Background technique [0002] Osteosarcoma is a malignant bone tumor common in children and adolescents. Chemotherapy is currently the main method to improve the survival rate of osteosarcoma patients. However, there are still 10% to 25% of osteosarcoma patients who have poor response to current chemotherapy regimens. It is tumor cells that are resistant to chemotherapy drugs. Apurine apyrimidinic endonuclease / redox factor antibody (anti-APE1) is a typical molecule with great structural and functional characteristics in biological macromolecules. It is composed of two relatively independent functional regions: DNA repair and redox. The key rate-limiting enzyme in the DNA base excision repair (BER) pathway can not only repair DNA damage caused by radia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/531G01N27/48
Inventor 仲召阳王东关伟梁伟戴楠卿毅
Owner THE THIRD AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIV OF PLA
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