Preparation of ELISA kit for detecting deoxynivalenol

Abstract

The invention relates to preparation of an ELISA kit for detecting deoxynivalenol. The kit with sensitive detection, accuracy, fastness, simple operation and strong specificity is suitable for determination of a large number of samples. The kit includes an ELISA plate coated with deoxynivalenol antigens, a deoxynivalenol standard sample, a deoxynivalenol antibody working solution, an enzyme-labeled?second antibody working solution, a substrate solution A, a substrate solution B, a stop solution, a concentrating sample weak solution and a washing concentrate. The principle of the deoxynivalenol detection kit is solid phase indirect competitive enzyme-linked immunosorbent assay. An extracted sample, the enzyme-labeled?standard second antibody working solution and the antibody working solution are added to corresponding enzyme-labeled holes; after incubation for a period of time, the substrate solution A and the substrate solution B are added into a washing plate; under the action of the enzyme, blue color occurs in the holes; and the stop solution adding is added in, and the color changes from blue to yellow. The degree of color is in inverse proportion relationship with the content of deoxynivalenol in the standard substance or sample. The method can be directly used for detecting deoxynivalenol in cereals and cereal products.

Description

technical field [0001] The invention belongs to the technical field of detection, in particular to an ELISA kit for quantitative detection of vomitoxin residues in grains and grain products. Background technique [0002] Mycotoxins are secondary metabolites produced by fungi. There are currently more than 300 known mycotoxins with different chemical structures, among which more than 30 metabolites produced by fungal strains are pathogenic to both animals and humans. According to FAO estimates, about 25% of the world's food crops are polluted by mycotoxins every year, causing economic losses of hundreds of billions of dollars each year. Today, as humans are increasingly concerned about the quality and safety of food hygiene, the contamination of mycotoxins has become a problem that cannot be ignored. The common and relatively harmful mycotoxins in my country's agricultural products and feed mainly include aflatoxin, deoxynivalenol, deoxynivalenol, zearalenone and ochratoxin ...

AI Technical Summary

Problems solved by technology

At present, most of the immunological detection kits for vomitoxin are imported, while the development of domestic kits for vomitoxin is still relatively backward

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