Gene, expression vector, expression method, expression cell and application of human papilloma virus (HPV) 16 E7E6 fusion protein

A fusion protein and expression carrier technology, applied in the field of medical biology, can solve problems affecting the effect of immunotherapy and achieve strong immunogenic effects
CN102002105AActive Publication Date: 2011-04-06中国疾病预防控制中心病毒病预防控制所
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
中国疾病预防控制中心病毒病预防控制所
Publication Date
2011-04-06

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Abstract

The invention provides a gene, an expression vector, an expression method, an expression cell and an application of a human papilloma virus (HPV) 16 E7E6 fusion protein. In the invention, recombinant adenovirus is adopted to fuse E6 and E7 protein genes which have no converting activity after modification, and the mammalian cell codon optimization and transformation is performed based on the amino acid sequences of the fusion protein to lead the codon to be expressed in the mammalian cells in a high level. In the invention, an E7E6 fusion protein mammalian cell codon optimization gene is designed successfully, the recombinant adenovirus capable of expressing the HPV16 E7E6 fusion proteins in a high level is successfully constructed, has strong immunogenic performance, can induce immunoreaction of specific T cells, and completely protects immune mice from attacking of anti-tumor cells. The invention can be used for searching and developing therapeutic vaccines of cervical cancer.
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Description

technical field

[0001] The invention belongs to the field of medical biotechnology. Specifically, the present invention relates to a gene expressing human papillomavirus (HPV) type 16 E7E6 fusion protein, expression vector, method and immunotherapy application. Background technique

[0002] A large number of research data prove that the high-risk human papillomavirus (humanpapillomavirus, HPV) infection of the reproductive tract is closely related to cervical cancer. HPV DNA can be found in 90% of cervical cancer patients, among which HPV16 accounts for 50%, which is the main pathogenic factor causing cervical cancer. Although the curative effect of surgery and radiotherapy is good for patients with early stage cervical cancer, radiotherapy and certain chemotherapy must be given on the basis of surgery for patients with advanced cervical cancer. Due to the huge side effects of radiotherapy and the insensitivity of cervical cancer to chemotherapy, 35% of patients still have...

Claims

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