Bivalent hand-foot-and-mouth disease inactivated vaccine and preparing method thereof

Abstract

The invention relates to the technical field of biology, and discloses two kinds of hand-foot-and-mouth disease virus strains and bivalent hand-foot-and-mouth disease inactivated vaccine prepared through the two kinds of hand-foot-and-mouth disease virus strains. A human intestinal tract 71 type virus strain CC-09 with the preservation number of CGMCC NO.7753 and a Coxsackie A16 type virus strain CC-16 virus with the preservation number of CGMCC NO.7752 are inoculated to Vero cells for expanding continuous cell culture to be cultivated; two kinds of virus culture solutions are obtained, and concentration, inactivation and purification are carried out after impurity removing to obtain two kinds of virus stock solutions; the two kinds of virus stock solutions are sterilized and mixed, adjuvant is adsorbed, and then the bivalent hand-foot-and-mouth disease inactivated vaccine is obtained. The bivalent hand-foot-and-mouth disease inactivated vaccine is high in immunogenicity, the cross inhibition function does not exist between the two viruses, and the hand-foot-and-mouth disease epidemic in China at present can be effectively prevented. The bivalent hand-foot-and-mouth disease inactivated vaccine is simple in preparing technology, low in production cost and suitable for large-scale production.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a vaccine and a preparation method thereof. Background technique [0002] Hand foot and mouth disease (HFMD) is an infectious disease in children, also known as exanthematous vesicular stomatitis. It mostly occurs in children under the age of 5, and can cause herpes on the hands, feet, and oral cavity. The main clinical symptoms of the disease are herpes or ulcers on the mucous membranes of the hands, feet, and oral cavity. A small number of children can cause myocarditis, pulmonary edema, aseptic meningoencephalitis and other complications. In some severely ill children, if the disease develops rapidly, it will lead to death. [0003] Hand, foot and mouth disease is an infectious disease caused by enteroviruses. Enteroviruses are highly contagious, spread widely, and spread quickly. The epidemic of the disease can occur throughout the year, and has the characteristics of periodic ...

AI Technical Summary

Problems solved by technology

Hand, foot and mouth disease caused by EV71 and CoxA16 has the characteristics of recessive infection and mild cases, the source of infection is difficult to find and control, children are generally susceptible, and there are many transmission routes, which are difficult to effectively block. High-efficiency and broad-spectrum antiviral drugs such as acyclovir, valacyclovir, and famciclovir are commonly used to shorten fever and skin lesion healing time, and relieve oral herpes pain. So far, there is no specific drug for the prevention of this disease

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