Portable electrochemical quantitative immunochromatography filtering paper as well as test paper and application thereof

An immunochromatography and electrochemical technology, applied in the field of biosensing, can solve the problems of operator hazards, increased detection costs, and long time consumption, and achieve the effects of good reliability, low cost, and simplified preparation

Active Publication Date: 2014-05-07
SOUTHWEST UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, detection and diagnosis techniques for biochemical samples, such as pathogens in blood and urine, or harmful pollutants in milk and fruit juice in food testing mainly include: radioimmunoassay (RIA), enzyme-linked immunoassay (ELISA), chemiluminescence ( CLIA) and other methods, but these methods have their own characteristics and shortcomings: radioimmunoassay technology is mature, the results are relatively accurate, and the linear range is wide, but the operation is cumbersome and time-consuming, and the radioactive markers will cause harm to the operator and will Environmental pollution has been gradually replaced by other methods
However, this method can only achieve semi-quantitative, can only indicate a general range, and cannot achieve accurate quantification, and because of the limitation of the sensitivity of colloidal gold, free protein cannot be detected, and abnormal samples need to be checked by other methods, which virtually increases the detection cost. It is difficult to promote in the market, and it is difficult to use it on a large scale

Method used

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  • Portable electrochemical quantitative immunochromatography filtering paper as well as test paper and application thereof
  • Portable electrochemical quantitative immunochromatography filtering paper as well as test paper and application thereof
  • Portable electrochemical quantitative immunochromatography filtering paper as well as test paper and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Preparation of portable electrochemical quantitative immunochromatography filter paper (direct method), a hydrophilic one-way channel 1 was formed on the filter paper by a paraffin printer, and the printed filter paper was baked on a hot plate at 120°C for 10 minutes, and then Cool at room temperature (18-25°C), set one end of the channel as the sample loading area 2, and the other end is superimposed with absorbent paper and set as the absorbent area 3 to control the flow of liquid on the filter paper, and set it between the sample loading area and the absorbent area Electrochemical detection area 4, coated with antigens or antibodies that can specifically bind to target substances (proteins, small molecular compounds, toxins) in the electrochemical detection area 4, to prepare immunochromatographic filter paper structures such as figure 1 as shown in a.

[0026] (2) Preparation of portable electrochemical quantitative immunochromatography filter paper (competition...

Embodiment 2

[0031] Portable Electrochemical Quantitative Immunochromatography Test Paper Direct Method Detection

[0032] The portable electrochemical quantitative immunochromatographic filter paper was prepared according to the method of Example 1, and the detection antibody human IgG was coated on the electrochemical detection area, and then detected after assembly. During the detection, 50 μL of the sample diluted with 1:1000 (volume ratio) HRP goat anti-human IgG antibody was dropped into the sample loading area, and entered the electrochemical detection area through the capillary action of the filter paper fiber. After 5-10 minutes, the electrochemical detection area Add 20-50 μL of phosphate buffer (the concentration of phosphate buffer is 0.01-0.05M, pH 7.2-7.4) into the detection reaction area for electrochemical impedance spectroscopy detection. 100,000 to 1, while adding a sample that does not contain HRP sheep anti-human IgG antibody to the sample area as a control, and other c...

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Abstract

The invention discloses portable electrochemical quantitative immunochromatography filtering paper as well as test paper and application of the electrochemical quantitative immunochromatography filtering paper. The immunochromatography filtering paper is formed by arranging a hydrophilic channel on hydrophilic printing filtering paper; a sample feeding region is arranged at one end of the channel and a water absorption region is arranged at the other end of the channel; an electrochemical detection region is arranged between the sample feeding region and the water absorption region; water absorption paper is arranged in the water absorption region; the electrochemical detection region is coated with an antigen or antibody for detecting a target substance; the portable electrochemical quantitative immunochromatography filtering paper is manufactured by immunochromatography filtering paper, a conductive film and a bottom plate; the test paper can be used for quantitatively analyzing immunoreaction and eliminating the non-specific interferences, and has the advantages of accurate result, accuracy in measurement and the like.

Description

technical field [0001] The invention belongs to the technical field of biosensing, relates to a portable electrochemical immunochromatographic filter paper, and also relates to a test paper containing the portable electrochemical immune chromatographic filter paper and an application of the test paper. Background technique [0002] At present, detection and diagnosis techniques for biochemical samples, such as pathogens in blood and urine, or harmful pollutants in milk and fruit juice in food testing mainly include: radioimmunoassay (RIA), enzyme-linked immunoassay (ELISA), chemiluminescence ( CLIA) and other methods, but these methods have their own characteristics and shortcomings: radioimmunoassay technology is mature, the results are relatively accurate, and the linear range is wide, but the operation is cumbersome and time-consuming, and the radioactive markers will cause harm to the operator and will Environmental pollution has been gradually replaced by other methods....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N27/26
CPCG01N33/54386G01N33/558
Inventor 余玲史转转李长明
Owner SOUTHWEST UNIVERSITY
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