Multiple PCR primer system and detection method for detecting lung cancer circulating free DNA (cfDNA) methylation as well as application

Abstract

The invention discloses a multiple PCR primer system and detection method for detecting lung cancer circulating free DNA (cfDNA) methylation as well as application. The multiple PCR system for detecting lung cancer cfDNA methylation as well as application includes a first round of multiple PCR primer sets and/or a second round of multiple PCR primers, wherein the first round of multiple PCR primersets contains 55 pairs of PCR primers with sequences shown in SEQ ID NO.1-SEQ ID NO.110 in a sequence listing, the sequences of the second round of multiple PCR primers are shown in SEQ ID NO.111-SEQID NO.112. The method for detecting lung cancer cfDNA methylation simultaneously detect 55 methylation sites related to lung cancer at one time, multiple PCR primers have little mutual interference,strong specificity, and high amplification efficiency, besides, the method can reduce the sample loss, reduce detection cost, improve detection efficiency, significantly increase the detection rate ofearly lung cancer by a high-throughput sequencing technology, and is suitable for popularization and application.

Description

technical field [0001] The invention relates to the field of molecular biology detection, in particular to a multiplex PCR primer system, detection method and application for detection of plasma free DNA methylation in lung cancer. Background technique [0002] Lung cancer has always been the cancer with the highest incidence and mortality in the world. The early diagnosis of lung cancer is mainly based on the high-risk factors in the medical history, combined with imaging, endoscopy and molecular biology to carry out early screening, so as to achieve the purpose of early detection and early treatment. However, these detection methods for early diagnosis of lung cancer also have obvious deficiencies: low-dose spiral CT (LDCT) has the risk of radiation; bronchial endoscopy is often invasive, and there are many false negatives; The problem of low precision and many false negatives. There are also methods such as puncture tissue biopsy, which are traumatic and can only be use...

AI Technical Summary

Problems solved by technology

However, these methods for early diagnosis of lung cancer also have obvious deficiencies: low-dose spiral CT (LDCT) has the risk of radiation; bronchial endoscopy is often invasive, and there are many false negatives; The problem of low precision and many false negatives

However, DNA methylation markers of lung cancer have not been widely used in the field of general health screening and companion diagnosis

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