Lung cancer screening associated serum LncRNA marker and application thereof

A marker and lung cancer technology, applied in the direction of DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of insufficient sensitivity, overdiagnosis, lack of lung cancer tissue specificity, etc., to achieve specificity and sensitivity high effect

Inactive Publication Date: 2017-09-15
曹巍
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Problems solved by technology

Low-dose spiral CT has high sensitivity in early lung cancer screening, but its high sensitivity also makes it have a high false positive rate in screening lung cancer, and there is "overdiagnosis", resulting in some unnecessary examinations a

Method used

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  • Lung cancer screening associated serum LncRNA marker and application thereof

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Embodiment Construction

[0013] The technical solution of the present invention will be described in detail below through specific examples.

[0014] The detection of the expression level of LncRNA whose nucleotide sequence is shown in SEQ ID NO.1 in serum is based on qRT-PCR technology, and the operation steps are as follows:

[0015] 1. Plasma RNA extraction (according to the kit instructions, mirVana TM PARIS TM Kit, AM1556)

[0016] 1. Before the experiment, put 2×Denaturing Solution in a 37°C water bath for 30 minutes to completely dissolve it;

[0017] 2. Take an appropriate amount of plasma (if less than 100 μL, make up to 100 μL with Cell Disruption Buffer), add an equal amount of 2×Denaturing Solution, vortex / oscillate to mix, and place on ice for 5 minutes;

[0018] 3. Add phenol / chloroform equal to the total volume, pay attention to absorb the chloroform in the lower layer of the reagent, vortex and mix for 30-60 seconds, room temperature 12000rpm, 5min;

[0019] 4. Carefully pipette th...

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Abstract

The invention relates to the field of biological detection, and concretely to a lung cancer screening associated serum LncRNA marker and an application thereof. The nucleotide sequence of the marker is represented by SEQ ID NO.1. Researches prove that the expression level of the LncRNA marker in lung cancer patients and pulmonary nodule patients is significantly higher that of healthy people, so the LncRNA marker can be used for the screening and the auxiliary diagnosis of early lung cancer, also can be used for the treatment effect evaluation, the drug resistance monitoring and prognosis assessment of the lung cancer, and has a specificity and a sensitivity as a novel lung cancer diagnosis molecular marker.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a serum LncRNA marker related to lung cancer screening and an application thereof. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world, and it has become the main cause of cancer-related death worldwide, accounting for 21.7% of all cancer-related deaths, seriously threatening human health and life. At present, although great progress has been made in the treatment of lung cancer, its pathogenesis is still unclear and there is a lack of effective early diagnosis methods. The vast majority of lung cancer patients diagnosed have already had tumor invasion and metastasis, so the prognosis of lung cancer is still poor , The 5-year survival rate is only about 17%. A large number of studies have shown that early diagnosis and early treatment of lung cancer is an effective way to reduce its mortality. Low-dose spiral CT has hig...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/113
CPCC12Q1/6886C12Q2600/178
Inventor 曹巍代敏常菁吴莉华田夫崔渊博吕朋举龚芬芬
Owner 曹巍
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