Exosome ecDNA biomarker detection reagent for early diagnosis of lung cancer, and use of exosome ecDNA biomarker detection reagent

A technology of biomarkers and detection reagents, applied in the field of exosome ecDNA biomarker detection reagents, can solve problems such as false positives and false negatives, achieve high accuracy, reduce false negatives, and low sample requirements.

Pending Publication Date: 2020-12-29
3D BIOMEDICINE SCI & TECH CO LTD
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Problems solved by technology

However, in actual use, the requirements for sample collection are relatively high, and it can only be used as a preliminary qualitative diagnosis; since diagnostic cytology mainly observes changes in cell morphology and cannot see changes in tissue structure, there are certain limitations, and it is easy to false negative or false positive

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  • Exosome ecDNA biomarker detection reagent for early diagnosis of lung cancer, and use of exosome ecDNA biomarker detection reagent
  • Exosome ecDNA biomarker detection reagent for early diagnosis of lung cancer, and use of exosome ecDNA biomarker detection reagent
  • Exosome ecDNA biomarker detection reagent for early diagnosis of lung cancer, and use of exosome ecDNA biomarker detection reagent

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Embodiment Construction

[0035] In order to understand the content of the present invention more clearly, it will be described in detail with reference to the drawings and embodiments.

[0036] The present invention uses detection reagents for exosome ecDNA biomarkers in human blood or body fluids to help improve the sensitivity and accuracy of early diagnosis of lung cancer, reduce false negatives, reduce missed diagnosis of early lung cancer patients, reduce additional suffering of patients, relieve family and social burden.

[0037] In the process of actually applying the present invention to the early diagnosis of lung cancer, it can be realized through the following steps:

[0038] S1. Collect blood or body fluid samples suspected of being lung cancer patients, separate and purify exosomes in the samples;

[0039] S2. Separating and purifying ecDNA in exosomes;

[0040] S3. Expression detection and correlation analysis of the combination of ecDNA biomarkers C-myc, EGFR and CyclinD1.

[0041] A...

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Abstract

The invention relates to an exosome ecDNA biomarker detection reagent for the early diagnosis of lung cancer. The biomarker comprises exosome ecDNA biomarkers C-myc, EGFR and CyclinD1, and is used asa biomarker for predicting early lung cancer. The invention provides and verifies that the advantages of rapidness, no harm, high accuracy and high specificity are achieved when the exosome ecDNA is used as a biomarker expression level for the early diagnosis of lung cancer for the first time, and thereby the detection reagent has important significance for the early diagnosis of lung cancer.

Description

technical field [0001] The invention relates to the technical field of early detection of diseases, in particular to an exosome ecDNA biomarker detection reagent for early diagnosis of lung cancer. Background technique [0002] Lung cancer is a malignant tumor with the highest morbidity and mortality worldwide. The early stage of lung cancer is hidden and usually asymptomatic, but most patients are already in the middle and late stages when they are first diagnosed, and have lost the opportunity for surgical resection. The five-year survival rate of advanced lung cancer patients is less than 5%, while the five-year survival rate of early lung cancer patients can reach more than 90%. Therefore, early diagnosis is an important opportunity for lung cancer patients to obtain a good prognosis. [0003] Exosome (exosome) is a 30-150nm size, extremely low-density extracellular nanoscale capsule structure, which is formed by cells through a series of regulatory processes such as "...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6851
CPCC12Q1/6886C12Q1/6851C12Q2600/124C12Q2563/107C12Q2531/113
Inventor 马跃张亚楠
Owner 3D BIOMEDICINE SCI & TECH CO LTD
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