Micro cell hollow fiber reactor
A reactor, hollow technology, applied in the isotropic direction fixed on/in the organic carrier, can solve the problem of unsatisfactory etc.
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specific Embodiment 1
[0018] Specific example 1: (comparison of hollow fiber reactor and plate culture)
[0019] The silicone tube 1 is 13cm long, with an inner diameter of 0.8cm and an outer diameter of 1.2cm. Silicone stopper 2 The thin end is 0.75cm, and the thick end is 1.35cm. The hollow fiber is made of polyacrylonitrile, the outer diameter is 0.110cm-0.120cm, and the molecular weight cut-off is 1000KDa.
[0020] Rat hepatocytes with a live rate higher than 90% were harvested by 10 6 The cells / ml density was inoculated in a mixture of 4-fold concentrated medium and rat tail collagen solution (1:3; v:v), injected into hollow fiber 3, and placed at 37°C for 10 minutes to solidify. Cut the hollow fiber 3 short, 8cm×12, put it into the silicone tube 1, add 5ml Williams’E medium containing 5% fetal bovine serum, put it in 37°C, 5% CO 2 Culture in an incubator.
[0021] The same batch of rat liver cells was divided into 0.8×10 6 Cells / ml was inserted into 5ml Williams'E medium containing 5% fe...
specific Embodiment 2
[0029] Specific embodiment 2: (hollow fiber reactor is used for the research of drug metabolism)
[0030] Silicone tube 1 is 13cm long, with an inner diameter of 0.8cm and 1.2cm. Silicone stopper 2 The thin end is 0.75cm, and the thick end is 1.35cm. Hollow fiber 3 polysulfone material, outer diameter 0.110cm-0.120cm, molecular weight cut-off 1000KDa.
[0031] Rat hepatocytes with a live rate higher than 90% were harvested by 10 6 The cells / ml density was inoculated in a mixture of 4-fold concentrated medium and rat tail collagen solution (1:3; v:v), injected into hollow fiber 3, and placed at 37°C for 10 minutes to solidify. Cut the hollow fiber 3 short, 8cm×12, put it into the silicone tube 1, add 5ml Williams’E medium containing 5% fetal bovine serum, put it in 37°C, 5% CO 2 Culture in an incubator.
[0032] The same batch of rat liver cells was divided into 0.8×10 6 Cells / ml was inserted into 5ml Williams'E medium containing 5% fetal bovine serum, and cultured on a pl...
specific Embodiment 3
[0037] Specific embodiment 3: (hollow fiber reactor is used for the research of drug hepatotoxicity)
[0038] The silicone tube 1 is 13cm long, with an inner diameter of 0.8cm and an outer diameter of 1.2cm. Silicone stopper 2 The thin end is 0.75cm, and the thick end is 1.35cm. Hollow fiber 3 polysulfone material, outer diameter 0.110cm-0.120cm, molecular weight cut-off 1000KDa.
[0039] Rat hepatocytes harvested with a viability higher than 90% were inoculated at a density of 106 cells / ml in a mixture of 4-fold concentrated medium and rat tail collagen solution (1:3; v:v), and injected into hollow fibers3,37 ℃ for 10 minutes to solidify. Cut the hollow fiber 3 short, 8cm×12, put it into the silicone tube 1, add 5ml Williams'E medium containing 5% fetal bovine serum, put it into a 37°C, 5% CO2 incubator for static culture.
[0040] After culturing for 12-18 hours, each group was replaced with a culture medium containing rifampicin 10mg / ml, isoniazid 15mg / ml and a culture m...
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