Micro cell hollow fiber reactor

A reactor, hollow technology, applied in the isotropic direction fixed on/in the organic carrier, can solve the problem of unsatisfactory etc.

Inactive Publication Date: 2005-03-02
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the large hollow fiber reactors used in industrial production obviously cannot meet this requirement.

Method used

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  • Micro cell hollow fiber reactor
  • Micro cell hollow fiber reactor
  • Micro cell hollow fiber reactor

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0018] Specific example 1: (comparison of hollow fiber reactor and plate culture)

[0019] The silicone tube 1 is 13cm long, with an inner diameter of 0.8cm and an outer diameter of 1.2cm. Silicone stopper 2 The thin end is 0.75cm, and the thick end is 1.35cm. The hollow fiber is made of polyacrylonitrile, the outer diameter is 0.110cm-0.120cm, and the molecular weight cut-off is 1000KDa.

[0020] Rat hepatocytes with a live rate higher than 90% were harvested by 10 6 The cells / ml density was inoculated in a mixture of 4-fold concentrated medium and rat tail collagen solution (1:3; v:v), injected into hollow fiber 3, and placed at 37°C for 10 minutes to solidify. Cut the hollow fiber 3 short, 8cm×12, put it into the silicone tube 1, add 5ml Williams’E medium containing 5% fetal bovine serum, put it in 37°C, 5% CO 2 Culture in an incubator.

[0021] The same batch of rat liver cells was divided into 0.8×10 6 Cells / ml was inserted into 5ml Williams'E medium containing 5% fe...

specific Embodiment 2

[0029] Specific embodiment 2: (hollow fiber reactor is used for the research of drug metabolism)

[0030] Silicone tube 1 is 13cm long, with an inner diameter of 0.8cm and 1.2cm. Silicone stopper 2 The thin end is 0.75cm, and the thick end is 1.35cm. Hollow fiber 3 polysulfone material, outer diameter 0.110cm-0.120cm, molecular weight cut-off 1000KDa.

[0031] Rat hepatocytes with a live rate higher than 90% were harvested by 10 6 The cells / ml density was inoculated in a mixture of 4-fold concentrated medium and rat tail collagen solution (1:3; v:v), injected into hollow fiber 3, and placed at 37°C for 10 minutes to solidify. Cut the hollow fiber 3 short, 8cm×12, put it into the silicone tube 1, add 5ml Williams’E medium containing 5% fetal bovine serum, put it in 37°C, 5% CO 2 Culture in an incubator.

[0032] The same batch of rat liver cells was divided into 0.8×10 6 Cells / ml was inserted into 5ml Williams'E medium containing 5% fetal bovine serum, and cultured on a pl...

specific Embodiment 3

[0037] Specific embodiment 3: (hollow fiber reactor is used for the research of drug hepatotoxicity)

[0038] The silicone tube 1 is 13cm long, with an inner diameter of 0.8cm and an outer diameter of 1.2cm. Silicone stopper 2 The thin end is 0.75cm, and the thick end is 1.35cm. Hollow fiber 3 polysulfone material, outer diameter 0.110cm-0.120cm, molecular weight cut-off 1000KDa.

[0039] Rat hepatocytes harvested with a viability higher than 90% were inoculated at a density of 106 cells / ml in a mixture of 4-fold concentrated medium and rat tail collagen solution (1:3; v:v), and injected into hollow fibers3,37 ℃ for 10 minutes to solidify. Cut the hollow fiber 3 short, 8cm×12, put it into the silicone tube 1, add 5ml Williams'E medium containing 5% fetal bovine serum, put it into a 37°C, 5% CO2 incubator for static culture.

[0040] After culturing for 12-18 hours, each group was replaced with a culture medium containing rifampicin 10mg / ml, isoniazid 15mg / ml and a culture m...

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Abstract

The micro hollow fiber cell reactor consists of silica gel tube of permeable silica gel material, permeable silica gel stopper and hollow fiber filament. Cell is embedded inside the hollow fiber filament with collagen gel, and the micro hollow fiber cell reactor has oxygen transferring and mass transferring capacity suitable for cell to survive and maintains the medicine metabolizing and other functions of cell. The micro hollow fiber cell reactor combines the advantages of miniature orifice plate and 3D bionic artificial liver reactor, maintains the bioactivity of animal cell. It may be used as in vitro mold of liver cell for the research of medicine metabolism and pharmacological and toxicological research and may be used in the lab research of cell culturing condition and cell product. It may be also used as the carrier for the research of animal cell culturing condition and lays the foundation for industrial production.

Description

Technical field [0001] The invention relates to a miniature cell hollow fiber reactor. Background technique [0002] The existing drug pharmacology and toxicology studies are based on animal models, in vitro primary cell models and continuous cell line models. Animal models have disadvantages such as long experimental period, low efficiency, large individual differences leading to large deviations in results, which are not conducive to animal protection, and cannot eliminate species differences, which is the fatal weakness of animal models. Although the continuous cell line has a simple culture method, high efficiency and low cost, there are still large differences in structure and function between the continuous cell line and the primary cultured cells, and cannot fully reflect the metabolism and toxicity of drugs in humans or animals. [0003] At present, researchers at home and abroad have begun to pay attention to the application of in vitro primary cell models in drug ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/04
Inventor 孟琴沈冲
Owner ZHEJIANG UNIV
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