Dialdehyde carboxymethyl cellulose-collagen frozen gel and preparation method thereof

A dialdehyde carboxymethyl and cryogel technology is applied in the field of preparation of biomedical materials to achieve the effects of high biocompatibility, easy adsorption and growth, and controllable biodegradation and absorption

Inactive Publication Date: 2010-09-29
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Examples

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Embodiment 1

[0024] Using fresh and healthy pigskin as raw material, the collagen with triple helical structure is prepared by enzymatic extraction, and then the dry collagen product is prepared after dialysis and freeze-drying.

[0025] Using sodium carboxymethyl cellulose as the raw material and sodium periodate as the oxidizing agent, the ratio of the two is 1:1, reacting at 35°C and pH 3.0 for about 4 hrs, and making it after alcohol precipitation, washing, drying and pulverization Dialdehyde carboxymethyl cellulose with an aldehyde content of 73.85%.

[0026] Weigh 10 parts of dry collagen product and add to 990 parts of water, and stir continuously for about 8 hrs at about 10°C to obtain a collagen solution with a mass fraction of about 1%. At the same time, 1 part of dialdehyde carboxymethyl cellulose was weighed and added to 999 parts of water, and stirred continuously at about 25°C for about 8 hrs to obtain a dialdehyde carboxymethyl cellulose solution with a mass fraction of abou...

Embodiment 2

[0028] Using fresh and healthy bovine Achilles tendon as raw material, the collagen with triple helical structure is prepared by enzymatic extraction, and then the dry collagen product is prepared after dialysis and freeze-drying.

[0029] Using sodium carboxymethyl cellulose as raw material, sodium periodate as oxidizing agent, the ratio of the two is 1:1, reacting at 35°C and pH value 2.0 for about 4hrs, after alcohol precipitation, washing, drying and crushing, it is prepared Dialdehyde carboxymethyl cellulose with an aldehyde content of 91.37%.

[0030] Weigh 10 parts of dry collagen product and add to 990 parts of water, and stir continuously for about 12 hours at about 10°C to obtain a collagen solution with a mass fraction of about 1%. At the same time, weigh 1 part of purified dialdehyde carboxymethyl cellulose and add it to 999 parts of water, and stir continuously for about 10 hrs at about 25°C to obtain a solution with a mass fraction of about 0.1% dialdehyde carbox...

Embodiment 3

[0032] Using fresh and healthy bovine Achilles tendon as raw material, the collagen with triple helical structure is prepared by enzymatic extraction, and then the dry collagen product is prepared after dialysis and freeze-drying.

[0033] Using sodium carboxymethyl cellulose as raw material, sodium periodate as oxidizing agent, the ratio of the two is 10:9, reacting at 45°C and pH value 3.0 for about 4hrs, after alcohol precipitation, washing, drying and crushing, it is prepared Dialdehyde carboxymethyl cellulose with an aldehyde content of 56.85%.

[0034]Weigh 10 parts of dry collagen product and add to 990 parts of water, and stir continuously for about 12 hrs at about 10°C to obtain a collagen solution with a mass fraction of about 1%. At the same time, weigh 10 parts of dialdehyde carboxymethyl cellulose and add it to 990 parts of water, and stir continuously at about 25°C for about 10hrs to obtain a solution with a mass fraction of about 1% of dialdehyde carboxymethyl c...

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Abstract

The invention provides dialdehyde carboxymethyl cellulose-collagen frozen gel and a preparation method thereof. The method comprises the following steps of: first uniformly mixing 1 to 3 mass percent of the aqueous solution of collagen and 0.01 to 1 mass percent of the aqueous solution of dialdehyde carboxymethyl cellulose; then injecting the mixed solution into a mold and storing the mixed solution in a low-temperature reactor at the temperature of -40 to 0 DEG C for 1 to 7 days; and finally, taking the obtained product out and slowly unfreezing the obtained product to obtain the dialdehyde carboxymethyl cellulose-collagen frozen gel. The dialdehyde carboxymethyl cellulose-collagen freezing gel prepared by the method is improved in mechanical property, thermal stability and enzymatic degradation resistance at the same time of maintaining the biological activity of collagen gel per se, has the advantages of porosity, hydrophilicity, water absorbing and preserving property, good market application prospect and the like, and is widely used in biomedical fields, such as biologic scaffolds, cell culture, drug delivery, tissue engineering, wound and burning treatment and the like.

Description

technical field [0001] The invention relates to the field of preparation of biomedical materials, in particular to a dialdehyde carboxymethylcellulose-collagen cryogel with good porosity, hydrophilicity and water absorption and water retention and a preparation method thereof. Background technique [0002] Hydrogel is a polymer material similar to living tissue. It is composed of insoluble but swellable polymer network and water, so it has formability, hygroscopicity, film-forming property, cohesiveness, large heat capacity, etc. performance, widely used in biomedical fields such as biological scaffolds, cell culture, drug controlled release, tissue engineering and trauma, burn treatment and hemostasis, among which the hydrogels used in tissue engineering are mainly divided into two categories: synthetic polymer hydrogels Glue and natural polymer hydrogel. [0003] Collagen is a structural protein of the extracellular matrix (ECM), and there should be at least one structura...

Claims

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Application Information

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IPC IPC(8): C08L89/00C08L89/06C08L1/26C08J3/24A61L27/26A61L27/24A61L27/20A61L27/56A61L31/04A61L31/14
Inventor 穆畅道李昌朋林炜李宏利李德富
Owner SICHUAN UNIV
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