The invention relates to a feed additive capable of reducing disease incidence rate of livestock and poultry. The feed additive is mainly prepared from the following raw materials in parts by mass: 10-15 parts of radix isatidis, 15-20 parts of honeysuckle flowers, 20-25 parts of dandelions, 15-20 parts of rheum officinale, 20-25 parts of weeping forsythia capsule, 25-30 parts of astragalus membranaceus, 15-20 parts of purslane herbs, 25-30 parts of Chinese thorowax roots, 15-20 parts of garlic, 15-20 parts of cortex phellodendri and 15-20 parts of radix scutellariae. A preparation method of the feed additive comprises the following steps of mixing the raw materials, crushing the mixed materials to obtain powder of 100 meshes or above, decocting the powder for 2h or above, performing concentration, and performing drying so as to obtain the feed additive. The feed additive disclosed by the embodiment of the invention is simple in formula of raw materials and mild in medicine properties, the remarkable effects of mutually matched specific plant extracts in the aspects of restraining and eliminating medicine resistance of medicine-resistant bacteria are utilized, the mutually matched specific plant extracts are used as a feed additive to be added into feeds for compound use and particularly have the effect of restraining the medicine resistance of escherichia coli in animal bodies, and besides, the incidence rate of related diseases of the livestock and the poultry is reduced.