3D printed mask and manufacturing technology thereof
A 3D printing and preparation technology, applied in the field of skin care products, can solve the problems of long time-consuming, inactivation, cumbersome process, etc., and achieve the effect of simple method and short time-consuming
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[0049] The present invention has no special limitation on the preparation method of the above-mentioned facial mask liquid, and the preparation method of the facial mask liquid known to those skilled in the art will suffice. In the present invention, preferably adopt following method to carry out the preparation of mask liquid:
[0050] mixing the hyaluronic acid aqueous solution with the freeze-dried powder of the stem cell growth factor, and then mixing with the aqueous solution of the honeysuckle extract and the honey aqueous solution to obtain a mixed solution;
[0051] The mixed solution is sterilized by filtration and concentrated to obtain a mask liquid.
[0052] In the present invention, hyaluronic acid is first prepared into an aqueous solution, and the concentration of the hyaluronic acid aqueous solution is 1-5 μg / ml, preferably 2-4 μg / ml. After obtaining the hyaluronic acid aqueous solution, dissolve the stem cell growth factor freeze-dried powder with the hyaluro...
Embodiment 1
[0075] Preparation of Stem Cell Growth Factor
[0076] Obtain primary mesenchymal stem cells from autologous adipose tissue by enzymatic digestion. The process is as follows: adipose tissue solution obtained by liposuction, rinsed with PBS (phosphate buffered saline) for 2 to 3 times, and added BSA with a final concentration of 1 wt%. (bovine serum albumin) and collagenase type 1 with a final concentration of 0.1wt%, shake in a shaking incubator at 37°C for 30min; then add X-VIVO15 containing 10% (volume percent concentration) FBS (fetal bovine serum) Serum-free medium was used to stop the digestion; after that, the undigested tissue was filtered through an 80-mesh mesh, and the filtrate was centrifuged at 400 g for 10 min; finally, the upper three layers were discarded, and the adipose stem cells in the lower layer were the primary mesenchymal stem cells isolated in this example.
[0077] Primary mesenchymal stem cells were inoculated in LONZA X-VIVO15 serum-free medium conta...
Embodiment 2
[0080] Preparation of honeysuckle extract
[0081] First take honeysuckle powder, add 10 times the mass of 50wt% ethanol, extract twice at 80°C for two hours each time, combine the immersion solution and filter; then the filtrate is concentrated under reduced pressure, ethanol is recovered, and the pH of the concentrated solution is adjusted to about 2 , extracted four times with 4 times the amount of ethyl acetate, combined the extracts; then added an appropriate amount of water to the extracts to wash twice, adjusted the pH to 6-7 with 40wt% NaOH, allowed the layers to stand, and took the water layer; Finally, the water layer is pressurized and concentrated to a thick paste, and air-dried at room temperature to obtain the honeysuckle extract.
[0082] The composition of the above-mentioned honeysuckle extract was analyzed, and the results were as follows: the content of neochlorogenic acid was 4 wt%, the content of chlorogenic acid was 10 wt%, the content of caffeic acid was...
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