A tooth mineralization solution and its mineralization method

A tooth and phosphate solution technology, applied in tooth protection coating, dentistry, dental preparations, etc., can solve the problems of long processing time, unstable mineralization effect, and difficulty in long-term preservation of mineralized solution, and achieves isolation of irritation and good effect. Beneficial for long-term storage and enhanced penetration

Active Publication Date: 2020-10-30
ZHEJIANG UNIV
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  • Application Information

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Problems solved by technology

[0010] The purpose of the present invention is to overcome the problems of long treatment time, unstable mineralization effect, need to continuously provide calcium and phosphorus sources, and difficulty in long-term preservation of mineralization solution in the tooth and collagen mineralization technology, and provide a tooth mineralization solution and its Methods for application to teeth and collagen mineralization

Method used

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  • A tooth mineralization solution and its mineralization method
  • A tooth mineralization solution and its mineralization method
  • A tooth mineralization solution and its mineralization method

Examples

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Embodiment 1

[0045] Dissolve 30g of calcium chloride and 3g of polyaspartic acid in 20ml of water, and adjust the pH to 7 to prepare Reagent A. Prepare reagent B according to the calcium-phosphorus ratio of 1.67, and adjust the pH to 7.

[0046] Preparation of demineralized dentin slices: prepare dentin slices with a size of about 3*3*2mm, etch with 37% phosphoric acid for 15 seconds, and rinse with deionized water for 30 seconds. Apply reagent A on the surface of dentin for 20 minutes and let it stand for 10 minutes. Then apply reagent B for 20 minutes and let stand for 10 minutes.

[0047] To prepare the model of monolayer recombinant type Ⅰ collagen: take 8 μL of rat tail type Ⅰ collagen fiber stock solution and dissolve it in 0.5 mL buffer solution (50 mM glycine, 200 mM potassium chloride, pH=9.2). Draw 3 μL of collagen solution and drop it on the tweezers, and place it in a 37°C incubator for more than 10 hours. After taking out 0.05% glutaraldehyde and crosslinking for 1 hour, rin...

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Abstract

The invention discloses a tooth mineralization liquid and a mineralization method thereof. The tooth mineralization liquid can be used for mineralizing collagen and teeth. The components of the mineralization fluid of the present invention are two parts placed separately, that is, reagent A comprising a complex of non-collagen analogues and calcium salts, and reagent B comprising phosphate. The pH value of reagent A and reagent B needs to be adjusted to 5-12 during the preparation of tooth mineralization solution of the present invention. The mineralization method of the tooth mineralization liquid of the present invention is to first smear the tooth surface with the reagent A and let it stand, and then use the reagent B to smear the tooth surface and let it stand, and then repeat the coating several times according to the need, so as to realize the tooth mineralization. In addition, the mineralization solution of this invention can also be used to achieve the biomimetic mineralization of collagen in the same way, including single-layer restructured collagen fibers, collagen gel and collagen sponge, soaked or floated in reagent A, then taken out and blotted dry with filter paper, and then Soak or float in reagent B to achieve biomimetic mineralization, and it can also be repeated several times.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to a tooth mineralization liquid and a method for applying it to teeth and collagen mineralization. Background technique [0002] It is well known that the structure of a tooth consists of an inner layer of dentin and an outer shell of enamel. Dentin is composed of a hierarchical, ordered arrangement of mineralized collagen. Hydroxyapatite is deposited orderly on the surface and inner interstitial regions of the collagen template. Minerals within these fibers are particularly important for the mechanical and biological properties of teeth and bones. However, the complexity of the structure makes dentin mineralization has always been a difficulty in biomimetic mineralization. There are also dentine tubules in the dentin, which communicate with the pulp. If factors such as acid erosion, abrasion, or gingival recession cause the loss of enamel or cementum and deminer...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K6/891A61K6/838A61K6/831A61K6/20
CPCA61K6/20A61K6/831A61K6/838A61K6/891
Inventor 傅柏平周子淮吴志芳张雷青赵维家
Owner ZHEJIANG UNIV
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