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High-strength collagen gel and preparation method thereof

A technology of collagen gel and collagen hydrogel, which is applied in the direction of pharmaceutical formulations, medical preparations with non-active ingredients, non-effective ingredients of polymer compounds, etc., and can solve biological safety hazards, collagen denaturation, and low mechanical strength of gels, etc. problem, achieve good compatibility, high degree of cross-linking, and easy industrial application

Active Publication Date: 2014-12-24
北京同威典石技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The traditional physical cross-linked collagen gel has the problem of denaturing collagen and the low mechanical strength of the gel caused by insufficient cross-linking, while the chemical cross-linking method enhances the chemical solvent and cross-linking agent residues in the collagen gel process. Potential biosafety hazard

Method used

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  • High-strength collagen gel and preparation method thereof
  • High-strength collagen gel and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] The collagen aqueous solution with a solid content of about 10% is injected into a specific mold, sealed after removing air bubbles, and irradiated with a high-energy electron beam of 25kGy to obtain an initial hydrogel with a compression modulus of 0.3MPa. When the hydrogel was immersed in water at 37°C, the gel would shrink by 40% in 24 hours, and the compressive modulus was 0.8MPa, which was 2.67 times that of the initial hydrogel; in 72 hours, the gel would shrink by 80%, and the compressive modulus was 1.5MPa, which increased to 5 times the size of the initial hydrogel.

Embodiment 2

[0037] Inject an aqueous collagen solution with a solid content of about 0.3% into the mold, defoam and seal it, and use 60 Coγ-rays were irradiated with 5kGy to obtain an initial hydrogel with a compressive modulus of 0.02MPa. The gel is placed in a phosphate buffer solution at a constant temperature of 37° C. for 24 hours, that is, the shrinkage exceeds 80%, and the compressive modulus increases to 0.06 MPa, which is 3 times that of the initial hydrogel.

Embodiment 3

[0039] Aqueous collagen (solid content about 5%) and hyaluronic acid aqueous solution are made into a blend solution with a total mass fraction of 3% according to the collagen / hyaluronic acid mass ratio of 98 / 2 (ultrasonic dispersion of tricalcium phosphate β-TCP), and the mixer is fully stirred , to remove air bubbles and inject into a specific mold, and irradiate 10kGy with electron beam quickly after sealing to obtain a ternary composite hydrogel. Put an electric heating ring (37°C) of appropriate size or a ring with warm water circulating in the center of the gel, keep the temperature for a period of time to induce the middle of the gel to shrink, and obtain a dumbbell-shaped material, which can be used as a tangible scaffold for bone tissue engineering and cultivate bone marrow Stromal cells are used in bone defect repair.

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Abstract

The invention relates to high-strength collagen gel. The collagen gel is characterized in that the high-strength collagen gel undergoes three-dimensional size shrinkage by immersing radiation cross-linked low-density collagen hydrogel in water or an aqueous solution for a period of time and maintaining a certain temperature; the compression modulus is increased by 2-5 times that of the low-density collagen hydrogel after self-shrinkage by solution immersion; and the high-strength collagen gel has a three-dimensional structure or high porosity, and the pore diameter is distributed in a gradient manner. The invention further relates to a method for preparing the high-strength collagen gel.

Description

technical field [0001] The invention relates to the technical field of medical equipment and its preparation, in particular to a collagen gel, and more particularly to a high-strength collagen gel and a preparation method thereof. Background technique [0002] Collagen is the main component of connective tissue and accounts for nearly one-third of the total protein in the human body. Its good biocompatibility and biodegradability make it a biomaterial that has been widely used and studied in clinical practice. Hydrogel is a three-dimensional network formed by cross-linking hydrophilic macromolecules, with high water content, low surface tension and excellent hydrophilicity; and composed of collagen, elastin, aminoglycans, proteoglycans and glycoproteins The extracellular matrix (ECM), a natural tissue scaffold constructed through high hydration, itself assumes the form of a hydrogel. Therefore, collagen hydrogel is considered to be an ideal material to replace ECM, and has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/28C08J3/075C08L89/00C08L5/08C08L5/02A61K47/42A61K47/36A61K47/38A61L27/26A61L27/52A61L27/54
Inventor 许零张雅晴张向梅
Owner 北京同威典石技术有限公司
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