Process of preparing propionic acid and coproducing vitamin B12 with Propionibacterium freudenreichii NX-4

A technology of Propionibacterium freundii and vitamins, applied in the direction of bacteria, fermentation, etc., can solve the problem of low yield of strains, achieve extensive culture conditions, convenient and simple operation, and improve the effect of product production rate

Inactive Publication Date: 2007-10-03
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also some researches on propionic acid in China, but the yield of strains is generally not high, and the highest fermentation level is 24g / L of propionic acid produced by Liang Huizhen and others from Tianjin University of Science and Technology with strain Propionic bacterium sp TQ-10

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Incline medium: glucose 10g / L, peptone 10g / L, beef extract 3g / L, NaCl 5g / L, agar 20g / L, pH6.9.

[0054] Seed medium: glucose 20g / L, peptone 5g / L, yeast extract 5g / L, NaCl 5g / L, pH6.9.

[0055] Fermentation medium: glucose 50g / L, corn steep liquor 20g / L, (NH 4 ) 2 SO 4 2g / L, K 2 HPO 4 5g / L, NaCl1g / L, pH6.9; 30ml of fermentation medium (ie fermentation medium) in a 25×200mm test tube, CaCO 3 0.5g / test tube, sterilized at 121°C for 15 minutes.

[0056] Inoculate two rings of Propionibacterium fresnerii NX-4 with the preservation number CCTCC No.M 207015 in the seed medium, and culture it at 33°C for 36 hours to obtain the seed liquid, and put the seed liquid into the cooling chamber according to the inoculation amount of 10% of the volume of the fermentation liquid. In the final fermentation broth, after culturing at 35°C for 42 hours, 1 g of dimethyl benzimidazole was added, and the cultivation was continued for 60 hours. The content of propionic acid in the final f...

Embodiment 2

[0058] Fermentation medium: glucose 60g / L, corn steep liquor 20g / L, (NH 4 ) 2 SO 4 2g / L, K 2 HPO 4 5g / L, NaCl1g / L, pH6.9; 300ml of fermentation broth in a 500ml Erlenmeyer flask, CaCO 3 5g / erlenmeyer flask, sterilized at 121°C for 15 minutes.

[0059] Prepare the seed culture medium, formula and culture method are the same as embodiment 1, insert the seed liquid into the fermented liquid after cooling according to the inoculum amount of 10% of the fermented liquid volume, after cultivating at 35 ℃ for 48h, add 1g of dimethylbenzimidazole, Continue to cultivate for 68 hours, and the content of propionic acid in the fermented liquid finally obtained is 28.8g / L; the fermented cells contain vitamin B 12 31mg / L.

Embodiment 3

[0061] Fermentation medium: glucose 20g / L, lactic acid 80g / L, corn steep liquor 40g / L, (NH 4 ) 2 SO 4 2g / L, K 2 HPO 4 5g / L, NaCl1g / L, pH6.9. 30ml of fermentation broth, CaCO 3 1g / test tube, sterilized at 121°C for 15 minutes.

[0062] Prepare seed culture medium, formula and culture method are the same as embodiment 1, seed liquid is inserted in the fermented liquid after cooling by the inoculum amount of 10% of fermented liquid volume, 35 ℃ cultivates 140 hours, contains propionic acid 38.5g / L.

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Abstract

The present invention discloses one kind of Propionibacterium freudenreichii NX-4 and the production process of propionic acid and vitamin B12 therewith. Propionibacterium freudenreichii NX-4 is preserved in the preservation number of CCTCC No. M 207015. Through inoculating Propionibacterium freudenreichii into culture medium containing carbon source, nitrogen source, inorganic salt and water, and static culturing, propionic acid or coproduced propionic acid and vitamin B12 may be prepared. The strain can utilize several kinds of carbon source and nitrogen source, and has extensive culture condition, simple operation, high propionic acid yield and low production cost.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and relates to a microbial strain Propionibacterium freudenreichii NX-4 with high propionic acid production (Propionic Acid), and its use for propionic acid and co-production of vitamin B 12 production. Background technique [0002] Propionic acid is an important fine chemical product and basic chemical raw material, which is widely used in food, feed, rubber, plastic, paint, coating, fragrance, medicine, pesticide, printing and other fields. [0003] The current industrial production method of propionic acid is chemical synthesis. In recent years, oil resources have become increasingly tense, prices have continued to rise, and the cost of producing propionic acid by chemical synthesis has risen. At the same time, the operating conditions of chemical synthesis are harsh, and consumers favor natural and organic products. The research on biological synthesis of propionic acid has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/42C12P7/52
Inventor 徐虹洪厚胜李莎杨丽熊强欧阳平凯
Owner NANJING UNIV OF TECH
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