50 results about "Propionibacterium freudenreichii" patented technology

The present invention discloses one kind of Propionibacterium freudenreichii NX-4 and the production process of propionic acid and vitamin B12 therewith. Propionibacterium freudenreichii NX-4 is preserved in the preservation number of CCTCC No. M 207015. Through inoculating Propionibacterium freudenreichii into culture medium containing carbon source, nitrogen source, inorganic salt and water, and static culturing, propionic acid or coproduced propionic acid and vitamin B12 may be prepared. The strain can utilize several kinds of carbon source and nitrogen source, and has extensive culture condition, simple operation, high propionic acid yield and low production cost.

The invention discloses a production method for fermenting ethylformic acid with immobilization microorganism, which comprises the following steps: proceeding with shake magnification culture fisher Propionibacteriaceae test glass inclined-plane bacteria; proceeding with seed tank magnification culture; centrifuging and collecting bacterial; making bacterial suspension; adding maize ear flour; absorbing; adding 4%-6% sodium alginate; adding 3%-5% polyvinyl alcohol; mixing 1-2 hours; dropping in 2%-4% calcium chloride solution in order to immobilize; filtering the fermentation liquor with 1-2mu m film; absorbing with anion exchange resin in static state; eluting resin; concentrating; crystallizing; getting more than 95% purity ethylformic acid product. The invention removes feedback inhibition of product, which improves the utilization ratio of bacteria, is fit for extraction of ethylformic acid, improves the ethylformic acid production level, and is fit for large scale industry production of ethylformic acid.

The invention provides a method for producing a propionic acid type mildewproof agent by utilizing a microbial fermentation method. The method comprises the following steps: carrying out solid fermentation or liquid fermentation on any one propionibacterium selected from Propionibacterium freudenreichii, a Propionibacterium shermanii or other dairy propionibacteria to; enabling propionic acid produced in the fermentation process to react with CaCO3 in a solid culture medium or a liquid culture medium to obtain a calcium propionate, or enabling the propionic acid to directly react with ammonia water fed in a liquid fermenting process to generate an ammonium propionate; and drying a solid base material to produce a solid propionic acid type mildewproof agent comprising the calcium propionate, or concentrating a fermentation liquor to produce a liquid propionic acid type mildewproof agent comprising the ammonium propionate. Because the used strain belongs to the diary propionibacteria and is publicly known as a safe strain, the propionate produced by the method is not required to be separated from propionic acid bacteria, and can be directly used for preventing feeds and grains from being mildewed. Compared with existing chemical methods, the propionic acid type mildewproof agent provided by the invention has advantages of wide raw material source, mild production condition, simplicity in producing process, low energy consumption, environmental friendliness and the like.

The invention belongs to the technical field of biological active bacterial preparations, particularly relates to a probiotic composition capable of obviously preventing and relieving the Alzheimer'sdisease, and further discloses a preparation prepared from the probiotic composition for preventing and relieving the Alzheimer's disease. The probiotic composition capable of obviously preventing andrelieving the Alzheimer's disease comprises lactobacillus plantarum Lp3a, bifidobacterium lactis Bla019, bifidobacterium longum BL5b, lactobacillus buchneri Lbu3, propionibacterium freudenreichii PrF01, propionibacterium shermanii, pediococcus acidilactici, clostridium butyricum and lactobacillus fermenti. The probiotic composition not only can obviously regulate and maintain intestinal microecology and control an in vivo toxin level but also can generate a certain quantity of short chain fatty acid, including acetic acid, propionic acid, butyric acid, lactic acid and the like to further relieve the related symptoms of the Alzheimer's disease. The probiotic composition has the efficacy of alleviating and relieving the symptoms of the Alzheimer's disease, also has the efficacy of soothingthe nerves and calming down, and also has a certain function for the depression, the anxiety disorder and the insomnia.

The invention provides a glutathione fermented mask formed through fermentation after enzymolysis of yeast paste. Active components in the mask are mainly nutritional components in lactic acid, propionic acid and thalluses which are naturally generated by lactic acid bacteria, propionibacterium freudenreichii and yeast. The aspiration of people for safety and natural beauty of people at present is met, market prospects are wide, and the mask has a certain contribution to improvement of life quality of people.

The invention relates to the use of microbes Lactobacillus rhamnosus LGG, ATCC 53103, Latobacillus rhamnosus LC705, DSM 7061, and Propionibacterium freudenreichii ssp. shermanii PJS, DSM 7067 in inhibiting yeast growth, for preventing and treating diseases caused by yeast and for relieving yeast-related symptoms in animals or humans.

The invention belongs to the technical field of pest control, and particularly relates to a biological control method for peanut root knot nematode. The biological control method for the peanut root knot nematode comprises the steps that a composite microbial agent with the peanut root knot nematode control effect is applied to peanut planting soil, and by means of the good insecticidal effect ofthe composite microbial agent, the peanut root knot nematode is subjected to biological control. According to the composite microbial agent, the selected composite microbial agent and plant extracts serve as effectively ingredients, the synergistic effect of lactobacillus rhamnosus, propionibacterium freudenii and bacillus amyloliquefaciens and the effects of water chestnuts and burdock zymolyte are facilitated, all the ingredients play the insecticidal action through coordination, both an indoor test and a field test prove that the composite microbial agent has a good insecticidal effect on the peanut root knot nematode, and the growth situation of peanuts cannot be affected at all.

The survival of lactic acid bacterial strains such as probiotic bacteria contained in yogurt etc. is improved. A lactic acid bacterium survival improver including a propionic acid bacterium fermentation product to improve the survival of a lactic acid bacterium. The propionic acid bacterium is a bacterium belonging to the genus Propionibacterium. The bacterium belonging to the genus Propionibacterium is Propionibacterium freudenreichii.

Methods and compositions are hereby disclosed for reducing the numbers of E. coli O157:H7, Salmonella or other pathogens in an animal. The methods include administering to the animal a lactic acid producing bacterium at a relatively low dosage in combination with a lactate utilizing bacterium, followed by administration of the lactic acid producing bacterium at a relatively high dosage. The disclosed methods help achieve pre-harvest food safety and enhance feed performance while keeping the total cost relatively low. The preferred lactic acid producing bacterium is Lactobacillus acidophilus / animalis and the preferred lactate utilizing bacterium is Propionibacterium freudenreichii.

The invention belongs to the technical field of microbial fermentation, and particularly relates to mixed fermentation liquor with improved bacteriostatic activity as well as a preparation method andapplication thereof. The mixed fermentation broth with improved bacteriostatic activity is obtained by mixed fermentation of lactobacillus plantarum and propionibacterium freudenreichsii subsp. shermanii. In the mixed fermentation process, two strains are added into a fermentation tank in proportion for anaerobic fermentation, growth of the two strains is controlled by supplementing glucose in thefermentation process, and fermentation is ended when the thallus amount in fermentation liquor reaches the maximum. A supernatant obtained by centrifuging the fermentation broth is the fermentation extract, and the fermentation broth liquid contains a plurality of organic acids and has a good inhibition effect on spoilage microorganisms such as penicillium, aspergillus and cladosporium.

The invention discloses a preparation method of humus for blueberry seedling cultivation and solves the problem that operation is quite troublesome due to the fact that in the existing blueberry cultivation process, acid increasing matter needs to be added regularly. The preparation method includes the steps that 1, water is added to grass wood materials, sandy soil and faeces, the mixture is mixed to be uniform, and no water seeps out of the mixed material when the material is held with hands, wherein the weight ratio of the grass wood materials to the sandy soil to the faeces is (10-30): 50: (10-20); 2, acetobacter pasteurianus and propionibacterium freudenreichii are added to the mixed material and stirred to be uniform; the total adding amount of the strains is at least 1*108 cuf / kg; 3, a film covers the mixed material, and fermentation is conducted for 5-8 days at the temperature of 25-28 DEG C; 4, the mixed material is cooled with cold air till the water content is 40-50%, then piled fermentation is conducted for 3-5 days at the temperature of 25-28 DEG C, and the humus is prepared. The preparation method has the advantages that the step of adding an acidic material is omitted, blueberry cultivation operation steps are simplified, and influences on growth of blueberries due to acidity difference are reduced.

The survival of lactic acid bacterial strains such as probiotic bacteria contained in yogurt etc. is improved. [Solving Means] A lactic acid bacterium survival improver including a propionic acid bacterium fermentation product to improve the survival of a lactic acid bacterium. The propionic acid bacterium is a bacterium belonging to the genus Propionibacterium. The bacterium belonging to the genus Propionibacterium is Propionibacterium freudenreichii. Propionibacterium freudenreichii is Propionibacterium freudenreichii ET-3 (FERM BP-8115).

Four new genes, and the enzymes that they encode, are disclosed, which are present in Propionibacterium freudenreichii, and which are involved in at least five steps in the biosynthetic pathway of vitamin B12. The four enzymes are: A: cobyrinic acid a, c-diamide synthase; B: a bifunctional enzyme, which is a cobinamide kinase and a cobinamide phosphate guanyl transferase; C: a cobalamin 5-phosphate synthase; and D: an adenosyl transferase. Genes encoding the four enzymes can be placed in Propionibacteria shuttle vectors and used to transform Propionibacteria hosts, in order to improve the production of vitamin B12 or a precursor thereof on an industrial scale during fermentation.

The invention discloses humus for blueberry cultivation and solves the problem that in an existing blueberry cultivation process, acid increasing matter needs to be added regularly, so that operation is quite troublesome. The humus for blueberry cultivation comprises grass wood materials, sandy soil, feces and strains, wherein the weight ratio of the grass wood materials to the sandy soil to the feces is (10-30): 50: (10-20); the strains include acetobacter pasteurianus and propionibacterium freudenreichii, and the adding quantity of the strains is at least 1*10<8> cuf / kg. The humus has the advantages that the step of adding the acid matter is omitted, the cultivation and operation steps are simplified, and influences of acidity difference on growth of blueberries are reduced.

The invention provides a probiotic grapefruit passion fruit tea and a preparation method thereof. According to the technical scheme, a propionibacterium freudenreichii-pediococcus pentosaceus fermented product used for blending a beverage is prepared at first, viable bacteria are removed, and meanwhile, the main nutrition effect of probiotic metabolite is basically maintained. The amount of viablebacteria in fermentation liquor approaches to zero, so that the flavor of the beverage is not influenced by the microbial growth and metabolism after the fermentation liquor is mixed with the beverage. Besides, according to the invention, odor ingredients in the fermentation liquor are specifically treated, main acidic odor substances, such as lactic acid and propionic acid, are fully decomposed,and all the fermentation medium components are selected from natural plants, so that the propionibacterium freudenreichii-pediococcus pentosaceus fermented product used for blending the beverage almost has no negative influence on the flavor of the beverage. The probiotic grapefruit passion fruit tea prepared according to the invention keeps an excellent flavor, has a certain intestinal tract probiotic effect and has a bright popularizing prospect.

Disclosed is a polypeptide including or consisting of the amino acid sequence SEQ id no:1, for use in the treatment or prevention of an inflammatory disease. It further encompasses a nucleic acid sequence encoding a polypeptide, a vector including a nucleic acid and a host cell including a nucleic acid sequence and / or a vector, for use in the treatment or prevention of an inflammatory disease. Also disclosed is a pharmaceutical composition or a food composition including a polypeptide, a nucleic acid sequence, a vector or a host cell and a pharmaceutically acceptable carrier or a dairy product, for the treatment of inflammatory disease. Additionally, a method for the screening of bacteria having immunomodulatory properties is disclosed.

The invention belongs to the technical field of pest prevention and control, particularly relates to a composite microbial agent for preventing and controlling Meloidogyne arenria, and further discloses a preparation method of the composite microbial agent. The composite microbial agent for preventing and controlling Meloidogyne arenria, adopting selected composite microbes and plant extract as effective components, facilitates the synergistic effects of Lactobacillus rhamnosus, Propionibacterium freudenreichii and Bacillus amyloliquefaciens and the effects of the enzymatic hydrolysate of water chestnuts and burdocks, all the components coordinate with each other to exert the insecticidal effect, and indoor test and field test of the composite microbial agent prove that the composite microbial agent of the invention has a good killing effect on the Meloidogyne arenria, and does not affect the growth of peanuts at all.

The invention discloses a method for promoting microbial denitrification. The microbial denitrification is promoted through the synergistic effect of paracoccus denitrificans and propionibacterium freundii. The method has the characteristics of shortening the denitrification process, increasing the denitrification rate, reducing the accumulation of intermediate products, avoiding secondary pollution and the like.

The invention includes methods and compositions for treating an animal to inhibit the incidence and growth of E. coli O157:H7 and other pathogenic bacteria. The treatment method comprises administering a therapeutically effective amount of Lactobacillus animalis or one or a combination of a number of other probiotic bacteria to an animal. An alternative treatment method comprises administering a therapeutically effective amount of a lactic acid producing bacterium such as Lactobacillus animalis in combination with a lactate utilizing bacterium such as Propionibacterium freudenreichii.

The invention discloses a deodorant special for a large-scale pig farm and application of the deodorant. The deodorant is prepared from a lactobacillus Raman culture, an ovoid bifidobacterium culture,a bifidobacterium longum culture, an intermediate streptococcus culture, a propionibacterium freudenreichii culture, an aspergillus itaconicus culture and a filamentous fungus fungicide; by adding the lactobacillus Raman culture, the ovoid bifidobacterium culture, the bifidobacterium longum culture, the intermediate streptococcus culture, the propionibacterium freudenreichii culture, the aspergillus aconitum culture and the filamentous fungus fungicide into a brown sugar water solution of which the concentration is 6 to 10 percent, the concentration of a lactobacillus Raman strain is up to 10<5> to 10<7> per ml, the concentration of an ovoid bifidobacterium strain is up to 10<6> to 10<8> per ml, the concentration of a bifidobacterium longum strain is up to 10<4> to 10<6> per ml, the concentration of an intermediate streptococcus strain is up to 10<7> to 10<9> per ml, the concentration of a propionibacterium freudenreichii strain is up to 10<4> to 10<8> per ml, the concentration of anaspergillus itaconicus strain is up to 10<4> to 10<7> per ml and the concentration of a filamentous fungus strain is up to 10<6> to 10<9> per ml. According to the deodorant disclosed by the invention,a live pig breeding house is treated through a spraying way, foul gaseous matters such as ammonia gas, hydrogen sulfide, methane, indole, volatile fatty acid, mercaptan can be fundamentally and quickly degraded, and adverse effects on health of live pigs cannot be generated.

The invention discloses a humus soil preparation method for blueberry cultivation. The problem that acid increase substances are required to be regularly added in the existing blueberry cultivation process and very troublesome operation is caused. The humus soil preparation method comprises the steps that step one, grass and wood materials, sand and excrement are evenly mixed with water, and the standard that no water seep from the mixed materials through hand holding is proper; the weight ratio of the grass and wood materials to the sand to the excrement is 10-30: 50:10-20; step two, acetobacter pasteurianus and propionibacterium freudenreichii are added to the mixed materials and are evenly stirred, wherein the total strain adding amount is at least 1 * 108 cuf / kg; step three, the mixed materials are covered with a film for fermentation, wherein the fermentation temperature is 25-28 DEG C, and the fermentation time is 8-10 days. The humus soil preparation method has the advantages of omitting the step of adding the acid increase substances, simplifying blueberry cultivation operating steps, reducing the blueberry growth influence caused by acidity difference and the like.

The invention relates to functional Daqu for brewing, and belongs to the technical field of Baijiu brewing. The Daqu disclosed by the invention consists of wheat, water, rhizopus oryzae bacterial liquid, microzyme and bacillus licheniformis liquid. The Daqu is characterized by further comprising trehalose and propionibacterium freudenreichii bacterial liquid. The Daqu obtained through steps of selecting materials, moistening the selected materials, and adding the rhizopus oryzae bacterial liquid, the microzyme, the bacillus licheniformis liquid and the propionibacterium freudenreichii liquid,and performing Daqu can increase yield, nucleotide concentration and tetramethylpyrazine content of Baijiu. The invention discloses the novel functional Daqu for a production and processing technologyof the Baijiu, and through solid state fermentation of pure foods with the novel functional Daqu, Baijiu which is healthier and better in flavor can be obtained.

The invention discloses a complex microbial inoculant. The complex microbial inoculant is characterized by comprising propionibacterium freudenreichii, bifidobacterium adolescentis and fallaxsaponin A. The used strains are easy to obtain, the cost is low, the process is controllable, the wound healing speed can be remarkably increased, the wound healing rate is remarkably increased, formation of wound tissue blood vessels can be promoted to accelerate wound healing, and a new method is provided for repairing chronic wounds in clinical treatment.

The invention discloses a preparation method of a pickled pitaya peel food and the pitaya peel pickled food prepared by the preparation method. The pickled pitaya peel food is prepared by the following step: pickling pitaya peels by using a bran pickle seasoning solution prepared from rice bran, water, lactobacillus plantarum, Propionibacterium freudenreichii, yeast and 10-hydroxyoctadecenoic acid, wherein a component gamma-dodecalactone generated by the bran pickle vegetable seasoning solution is mixed with bitter free amino acids and bitter peptides generated by proteolysis of vegetable proteins, and bitter taste of the bitter peptides is covered by aromatic components of the gamma-dodecalactone, so that the purpose of debitterizing is achieved.

The invention includes methods and compositions for treating an animal to inhibit the incidence and growth of E. coli O157:H7 and other pathogenic bacteria. The treatment method comprises administering a therapeutically effective amount of Lactobacillus acidophilus or one or a combination of a number of other probiotic bacteria to an animal. An alternative treatment method comprises administering a therapeutically effective amount of a lactic acid producing bacterium such as Lactobacillus acidophilus in combination with a lactate utilizing bacterium such as Propionibacterium freudenreichii.

The invention discloses a production method for fermenting ethylformic acid with immobilization microorganism, which comprises the following steps: proceeding with shake magnification culture fisher Propionibacteriaceae test glass inclined-plane bacteria; proceeding with seed tank magnification culture; centrifuging and collecting bacterial; making bacterial suspension; adding maize ear flour; absorbing; adding 4%-6% sodium alginate; adding 3%-5% polyvinyl alcohol; mixing 1-2 hours; dropping in 2%-4% calcium chloride solution in order to immobilize; filtering the fermentation liquor with 1-2mu m film; absorbing with anion exchange resin in static state; eluting resin; concentrating; crystallizing; getting more than 95% purity ethylformic acid product. The invention removes feedback inhibition of product, which improves the utilization ratio of bacteria, is fit for extraction of ethylformic acid, improves the ethylformic acid production level, and is fit for large scale industry production of ethylformic acid.