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Complex microbial inoculant and application thereof to skin repair

A compound bacterial agent and seed technology, applied in skin diseases, medical preparations containing active ingredients, drug combinations, etc., can solve the problems of limited donor sources, skin tissue loss, immune rejection, etc., to improve the healing rate, promote The formation of blood vessels and the effect of accelerating wound healing

Active Publication Date: 2020-07-28
吉安诺惠诚莘科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Trauma, extensive burns, diabetes or other chronic diseases can cause severe loss of skin tissue. At present, autologous, allogeneic skin transplantation or artificial substitutes through tissue engineering are commonly used in clinical repair, but these methods have new donor areas. Issues such as trauma, limited donor sources, and immune rejection

Method used

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  • Complex microbial inoculant and application thereof to skin repair
  • Complex microbial inoculant and application thereof to skin repair
  • Complex microbial inoculant and application thereof to skin repair

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A kind of composite microbial agent, it comprises:

[0043] Propionibacterium freunderi, Bifidobacterium adolescent, and saponin A of chrysanthemum.

[0044] The method for preparing fermented liquid by said composite microbial agent is as follows:

[0045] (1) Preparation of Propionibacterium frescheri fermented extract:

[0046] Use the inoculation loop to inoculate the preserved strains into the MRS liquid medium for activation, and then inoculate a single clone into the seed medium to prepare the seed liquid: the culture temperature is 30°C, and the culture time is 24 hours, then it can be used as 5.8* 10 7 (CFU / ml) Propionibacterium frescherii seed liquid;

[0047] Take 10% of the seed liquid and inoculate it into the liquid fermentation medium 1, the culture conditions are: temperature 37°C, culture time 20 hours, the formula of the liquid medium 1, according to the mass percentage: 0.5% of chrysanthemum saponin A, 1% of glucose , fructose 1%, trehalose 1%, co...

Embodiment 2

[0057] Comparison: Only Propionibacterium freunderi and chrysanthemum saponin A were used.

[0058] Preparation of Propionibacterium freundii fermentation extract:

[0059] Use the inoculation loop to inoculate the preserved strains into the MRS liquid medium for activation, and then inoculate a single clone into the seed medium to prepare the seed liquid: the culture temperature is 30°C, and the culture time is 24 hours, then it can be used as 5.8* 10 7 (CFU / ml) Propionibacterium frescherii seed solution;

[0060] Take 10% of the seed liquid and inoculate it into the liquid fermentation medium 1, the culture conditions are: temperature 37°C, culture time 20 hours, liquid medium 1 formula, according to the mass percentage: chrysanthemum saponin A5, glucose 1%, fructose 1% %, trehalose 1%, corn steep liquor (corn steep liquor) 6%, K 2 HPO 4 0.8%, (NH4) 2 SO 4 0.2%, Tween 0.1%, the rest is distilled water, pH 7.0, and the fermentation product is obtained after fermentation...

Embodiment 3

[0064] Contrast: Only use the fermentation extract of Bifidobacterium adolescentis and the saponin A of chrysanthemum.

[0065] Preparation of Bifidobacterium adolescentis fermentation extract:

[0066] Use the inoculation loop to inoculate the preserved strains into the MRS liquid medium for activation, and then inoculate a single clone into the seed medium to prepare the seed liquid: the culture temperature is 30°C, and the culture time is 24 hours, then it can be used as 5.3* 10 7 (CFU / ml) Bifidobacterium adolescentis seed liquid;

[0067] Take 10% of the seed liquid and inoculate it into the liquid fermentation medium 2, the culture conditions are: temperature 37°C, culture time 20 hours, the formula of the liquid medium 2: 0.5% of Palisporin A, 5% of glucose, fructooligosaccharides 8%, corn steep liquor (corn steep liquor) 6%, milk 10%, tryptone 5%, yeast powder 10%, ammonium citrate 2%, K 2 HPO 4 1%, (NH4) 2 SO 4 0.2%, Tween 0.1%, the rest is distilled water, pH 6....

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Abstract

The invention discloses a complex microbial inoculant. The complex microbial inoculant is characterized by comprising propionibacterium freudenreichii, bifidobacterium adolescentis and fallaxsaponin A. The used strains are easy to obtain, the cost is low, the process is controllable, the wound healing speed can be remarkably increased, the wound healing rate is remarkably increased, formation of wound tissue blood vessels can be promoted to accelerate wound healing, and a new method is provided for repairing chronic wounds in clinical treatment.

Description

technical field [0001] The invention relates to a composite microbial agent and its application in skin repair, and belongs to the technical field of microbial strain bacterial agents. Background technique [0002] The skin covers the surface of the animal body and is composed of stratified squamous epithelium and hoof tissue, rich in blood vessels, nerves, hair follicles, lymphatic vessels, sweat glands and other tissues, and is the first natural barrier between direct contact with the external environment It can protect the animal body from external stimuli including physical and chemical damage, microbial invasion, ultraviolet rays, etc., and can protect the body's circulation and metabolic stability. It has very important physiological significance for the animal body. The skin is also the largest organ of the body. one. [0003] Trauma, extensive burns, diabetes or other chronic diseases can cause severe loss of skin tissue. At present, autologous, allogeneic skin tran...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61K35/745A61P17/02A61K31/704
CPCA61K35/74A61K35/745A61K31/704A61P17/02A61K2300/00Y02A50/30
Inventor 易修元
Owner 吉安诺惠诚莘科技有限公司
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