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Production method of immobilized microorganism fermenting propionic acid

A technology of immobilized microorganisms and production methods, applied in the direction of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., can solve the problems of easy decomposition by microorganisms, influence of cell activity, poor mass transfer performance, etc., and achieve the reduction of acetic acid Existence, usage reduction, and emission reduction effects

Inactive Publication Date: 2010-05-19
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Natural polymer gel carriers are generally non-toxic to organisms, have good mass transfer performance, but low strength, are easily decomposed by microorganisms under anaerobic conditions, and have a short lifespan; synthetic polymer gel carriers generally have higher strength, but lower mass transfer performance. Poor, affects cell viability during cell fixation

Method used

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  • Production method of immobilized microorganism fermenting propionic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Bacterial species and medium used Table 1

[0030] serial number

Bacteria name (Chinese, Latin)

Numbering

Incline medium

1

Propionibacterium freunderi

Propionibacteria

freudenreichii

IFFI.10019

Basic medium (g / L): peptone 10,

Sucrose 20, Yeast Extract 10, Ammonium Phosphate 5

(pH=7.2-7.4)

[0031] The method for producing propionic acid fermented by immobilized propionic acid bacteria comprises the following steps: (1) culture of bacteria (2) immobilized propionic acid bacteria frescheri (3) liquid submerged fermentation (4) extraction process of propionic acid:

[0032](1) Wherein the bacterial classification cultivation procedure: think that the production bacterial classification adopts Propionibacteria freudenreichii test tube slant bacterial classification, shakes the flask amplification cultivation, the seed tank enlargement cultivation method. Incline culture: basic medium (g / L): 10 pep...

Embodiment 2

[0037] Bacterial species and medium used Table 1

[0038] serial number

Bacteria name (Chinese, Latin)

Numbering

Incline medium

[0039] 1

Propionibacterium freunderi

Propionibacteria

freudenreichii

IFFI.10019

Basic medium (g / L): peptone 10,

Sucrose 20, Yeast Extract 10, Ammonium Phosphate 5

(pH=7.2-7.4)

[0040] (1) Strain cultivation process: adopt Propionibacteria freudenreichii test tube slant bacterial classification→shake flask amplification→seed tank expansion cultivation method for production strain. Cultivation of slant strains: use the corresponding culture medium and add 25g agar for the above strains, culture at 35°C for 48 hours, put them in a 4°C refrigerator for storage; the shake flask culture conditions: temperature 40°C, speed 180 rpm, Time 30h; seed tank cultivation conditions: temperature 40°C, ventilation 13m 3 / h, tank pressure 0.09MPa, time 60 hours.

[0041] (2) Pr...

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Abstract

The invention discloses a production method for fermenting ethylformic acid with immobilization microorganism, which comprises the following steps: proceeding with shake magnification culture fisher Propionibacteriaceae test glass inclined-plane bacteria; proceeding with seed tank magnification culture; centrifuging and collecting bacterial; making bacterial suspension; adding maize ear flour; absorbing; adding 4%-6% sodium alginate; adding 3%-5% polyvinyl alcohol; mixing 1-2 hours; dropping in 2%-4% calcium chloride solution in order to immobilize; filtering the fermentation liquor with 1-2mu m film; absorbing with anion exchange resin in static state; eluting resin; concentrating; crystallizing; getting more than 95% purity ethylformic acid product. The invention removes feedback inhibition of product, which improves the utilization ratio of bacteria, is fit for extraction of ethylformic acid, improves the ethylformic acid production level, and is fit for large scale industry production of ethylformic acid.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation engineering, in particular to a method for producing propionic acid by fermentation of immobilized microorganisms. Background technique [0002] Propiornic acid (hereinafter referred to as PA) and its salts are ideal feed antifungal agents, and also have important uses in the fields of food, medicine, spices, herbicides and biodegradable plastics. As a major feed production country in the world, my country has a large demand for propionic acid, and it shows a trend of increasing year by year. [0003] Immobilized cell technology is a biotechnology that emerged in the 1970s. The so-called immobilized cell technology refers to the method of locating free cells in a limited space area by physical or chemical means, and keeping them catalytically active for repeated use. After the cells are immobilized, it has the advantages of high cell density, fast reaction speed, strong poison res...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/52C12R1/01
Inventor 王德培丁友昉高年发
Owner TIANJIN UNIV OF SCI & TECH
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