Corynebacterium glutamicum and method for preparing L-ornithine and salts thereof by using same

A technology of Corynebacterium glutamicum and ornithine, applied in the field of fermentation engineering, can solve the problems of low yield, many reaction steps, difficulty in large-scale production, etc., and achieves simple fermentation process, extensive culture conditions, and low production cost. Effect
CN101955901AActive Publication Date: 2011-01-26NANJING UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING UNIV OF TECH
Publication Date
2011-01-26

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Abstract

The invention belongs to the field of fermentation engineering and discloses corynebacterium glutamicum and a method for preparing L-ornithine and salts thereof by using the same. The corynebacterium glutamicum is corynebacterium glutamicum 1006 with the collection number of CGMCCNo.3663. The method comprises the following steps of: culturing the corynebacterium glutamicum in a culture medium containing carbon sources, nitrogen sources and inorganic salts at the temperature of between 28 and 30 DEG C to generate L-ornithine fermentation liquor; removing the thalli of the fermentation liquor by using ceramic membranes of 1 to 15 ten thousand Dalton; separating the fermentation liquor by using cation exchange resins to obtain L-ornithine solution; and decoloring and crystallizing the L-ornithine solution by a hydrochloric acid and ethanol method to obtain L-ornithine hydrochloride. The method has the advantages of simple thallus cultivation and contribution to industrialized mass production. The accumulation of the L-ornithine prepared from the corynebacterium glutamicum reaches 35 to 45g / L.
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Description

technical field

[0001] The invention belongs to the field of fermentation engineering, and relates to a high-yield L-ornithine Corynebacterium glutamicum and a method for preparing L-ornithine and its salt by using the bacteria. Background technique

[0002] L-Ornithine is one of the ubiquitous amino acids in living organisms. It was discovered by Jeffrey in 1877 in the hydrolyzate of bird urine fed with benzoic acid. L-ornithine is an important metabolic compound in living cells. It is a component of bacterial cell membranes and polypeptide antibiotics. It mainly participates in the urea cycle in organisms and is used for citrulline, arginine, proline, The biosynthesis of polyamines plays an important role in the excretion of ammoniacal nitrogen in the body. In recent years, the development of L-ornithine products at home and abroad has gradually heated up, and the market prospect has become increasingly broad.

[0003] L-ornithine hydrochloride is an important metabolic ...

Claims

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