Method utilizing fermentation to improve gamma-aminobutyric acid content of brown rice

A technology of GABA and brown rice, applied in the direction of microorganism-based methods, fermentation, biochemical equipment and methods, etc.

Inactive Publication Date: 2014-11-26
INST OF AGRO PROD PROCESSING SCI & TECH SICHUAN ACAD OF AGRI SCI
View PDF3 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The report uses millet bran as raw material to increase the added value of millet bran, but the scope of use of millet bran is different from that of brown rice.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Pick a ring of Lactobacillus brevis P-14 with an inoculation loop and inoculate it into 5mL MRS liquid medium for activation and culture for 24 hours, then inoculate it into MRS liquid medium with an inoculation amount of 1mL / 100mL and cultivate it until the OD600 value of the bacterial solution is 1.12, 6000 rpm / centrifuge for 5 minutes to collect the thalli, wash 2 times with sterile normal saline, and resuspend with sterile normal saline 1 / 10 times the volume of the original culture solution to prepare a lactic acid bacteria seed starter.

[0040] Brown rice is crushed and passed through a 60-mesh sieve, brown rice flour and water are mixed at a ratio of 10g:90mL, and sodium glutamate is added to the mixture of brown rice and water. After mixing 10g of brown rice with 90mL of water, add 1g of sodium glutamate, and mix well Afterwards, sterilize at 121°C for 30 minutes, inoculate 5mL of lactic acid bacteria seed starter for every 10g of brown rice, 90mL of water, and...

Embodiment 2

[0043] Pick a ring of Lactobacillus brevis P-14 with an inoculation loop and inoculate it into 5mL MRS liquid medium for activation and culture for 24 hours, then inoculate it into MRS liquid medium with an inoculation amount of 1mL / 100mL and cultivate it until the OD600 value of the bacterial solution is 1.12, 6000 rpm / centrifuge for 5 minutes to collect the thalli, wash 2 times with sterile normal saline, and resuspend with sterile normal saline 1 / 10 times the volume of the original culture solution to prepare a lactic acid bacteria seed starter.

[0044] Brown rice is crushed and passed through a 60-mesh sieve. The sieved brown rice flour is mixed with water at a ratio of 10g:90mL. After fully mixing, it is sterilized at 121°C for 30 minutes. Every 10g of brown rice and 90mL of water mixture is inoculated with 5mL of lactic acid bacteria seed starter. ℃ fermentation for 3 days.

[0045] After the fermentation is over, the brown rice has its unique fermented flavor and...

Embodiment 3

[0047] Use an inoculation loop to pick a ring of GABA-free lactic acid bacteria and inoculate it into 5mL MRS liquid medium for activation and culture for 24 hours, then inoculate it into MRS liquid medium with an inoculation amount of 1mL / 100mL and cultivate until the OD600 value of the bacteria solution is 1.21, 6000 rpm / separation Collect the thallus for 5 minutes, wash twice with sterile normal saline, and resuspend with 1 / 10 times the volume of the original culture solution in sterile normal saline to prepare a lactic acid bacteria seed starter.

[0048] Brown rice is crushed and passed through a 60-mesh sieve, brown rice flour and water are mixed at a ratio of 10g:90mL, and sodium glutamate is added to the mixture of brown rice and water. After mixing 10g of brown rice with 90mL of water, add 1g of sodium glutamate, and mix well After sterilizing at 121°C for 30 minutes, inoculate 5mL of GABA-free lactic acid bacteria seed starter for every 10g of brown rice, 90mL of wate...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method utilizing fermentation to improve the gamma-aminobutyric acid content of brown rice, and aims to solve the problems that the gamma-aminobutyric acid content is low in brown rice, and the conventional fermentation technology is complicated, is not suitable for increasing the gamma-aminobutyric acid content in brown rice, and cannot increase the taste and nutritional and healthcare value of brown rice. The method grafts a lactobacillus P-14 strain having a high yield of gamma-aminobutyric acid (GABA) onto brown rice or brown rice, which has been added with monosodium glutamate, to carry out fermentation so as to obtain fermented brown rice rich in GABA. The P-14 strain is isolated from traditional pickled vegetables, the fermentation raw material is sterilized brown rice, and the fermentation products can reach the food safety grade. Through the fermentation treatment, the nutrition and healthcare value of the brown rice are both improved in different levels, and the fermented brown rice can satisfy the nutritional and healthcare requirements on food.

Description

Technical field: [0001] The invention relates to a method for increasing gamma-aminobutyric acid of brown rice by fermentation of lactic acid bacteria. Background technique: [0002] γ-Aminobutyric acid (GABA) is an important non-protein amino acid, which has important physiological functions such as enhancing brain vitality, calming the nerves, regulating hormone secretion, improving lipid metabolism, lowering blood pressure, treating epilepsy and improving menopausal syndrome. GABA is widely distributed and exists in animals, plants and microorganisms, and has trace amounts in various cereals, vegetables, fruits and mushrooms. GABA can be prepared by chemical methods, plant enrichment methods and microbial methods. Compared with the former two, the microbial fermentation method has the advantages of mild conditions and relatively simple equipment requirements. [0003] At present, soaking and germination are mainly used to enrich γ-aminobutyric acid in brown rice in C...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00C12R1/24
Inventor 朱宇董玲姚英政黎剑
Owner INST OF AGRO PROD PROCESSING SCI & TECH SICHUAN ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap