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Lipopeptide-producing bacillus pumilus and application thereof

A technology of Bacillus pumilus and lipopeptide, which is applied in the field of lipopeptide-producing Bacillus pumilus, can solve the problems of poor water solubility, long biodegradation cycle, and slow bioremediation process, etc.

Active Publication Date: 2009-10-21
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the poor water solubility of most pesticides (such as synthetic pyrethroid insecticides such as kungfuthrin) and the small contact surface area with microorganisms during the biodegradation process, the bioremediation process is slow. A major factor in the long degradation cycle

Method used

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  • Lipopeptide-producing bacillus pumilus and application thereof
  • Lipopeptide-producing bacillus pumilus and application thereof
  • Lipopeptide-producing bacillus pumilus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Add 20mg·L to LB medium -1 Fe 2+ and Ca 2+ Can significantly promote the growth of Y8A and the production of surfactants; 10g L -1 Lactose can significantly promote the production of surfactants; strain Y8A has a strong ability to grow and produce surfactants in the range of 20-30°C and pH5-12; in a 250mL Erlenmeyer flask, liquid seeds are inoculated at 1% Add the amount to 50mL LB medium (add 10g·L -1 Lactose, 20mg·L -1 Fe 2+ and Ca 2+ , pH7.5), cultured on a shaker at 170 rpm at 30°C for 24h. After the fermentation is over, measure the surface tension value of the bacterial fermentation broth, and the value can reach a minimum of 23.5mN m -1 .

Embodiment 2

[0061] The oil-contaminated soil was air-dried, sieved and mixed, sterilized at 121°C for 30 minutes, and dried. Divided into six groups for comparative experiments, each group designed three parallels: (A) adding lipopeptide-producing strain Y8A bacterial liquid, (B) adding alkane-degrading bacteria Chryseobacterium sp.ATCC 11642), (C) adding Aromatic hydrocarbon-degrading bacteria Sphingomonas chlorophenolica ATCC 39723) bacterial liquid, (D) added Y8A and Chryseobacterium strain bacterial liquid, (E) added Y8A and Sphingomonas chlorophenolic bacteria solution, (F) adding Y8A, Chryseobacterium strains and Sphingomonas chlorophenes bacteria solution. The inoculation amount is 1.1×10 8 a·g -1 , mixed well, and the soil without bacterial solution was used as the control (CK). Cover the surface of the container with a plastic film, pierce the holes for ventilation, incubate at 30°C for 30 days, and measure the residual crude oil in the soil by gravimetric method. The test re...

Embodiment 3

[0065] In 1 / 10LB containing kaufthrin (final concentration is 100mg·L -1 ) into the culture medium of kaufthrin-degrading bacteria Bacillus cereus ATCC7039 and the lipopeptide-producing strain Y8A of the present invention at the same time, the inoculation ratio is 1:1, and the inoculation amount is 1%. Determination of fermentation broth OD 600 and kung fu permethrin residues. Compared with the growth of Bacillus cereus strains and the degradation rate of kaufthrin when cultured alone, the degradation rate of kaufthrin was significantly accelerated in mixed culture, and the maximum degradation amount was reached in 34 hours, which was 7 hours earlier than that without Y8A. hour, the degradation process was shortened by nearly 1 / 6. After 2 days of cultivation, the measurement results showed that when the kaufthrin-degrading bacterium Bacillus cereus was cultured alone, the degradation rate of kaufrin was 38.7%, and when Bacillus cereus and Y8A were co-cultured, the degradatio...

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Abstract

The invention discloses a lipopeptide-producing bacillus pumilus, and classification thereof is named to be Bacillus pumilus Y8A which is preserved by China Center for Type Culture Collection (CCTCC) in Wuhan University with the preservation number of CCTCCM 209108. The invention also discloses an application of the lipopeptide-producing bacillus pumilus in promoting the micro-biological degradation process of petroleum and / or cyhalothrin pesticide. Bacterial strain Y8A of the invention can produce lipopeptide biosurfactant with high efficiency and stability, thereby effectively promoting the micro-biological degradation process of petroleum and / or cyhalothrin pesticide degrading bacteria. Bacterial strain Y8A with the function of efficiently producing lipopeptide is expected to work as a new-generation bacteria and cosolvent for antibiotic production and, is widely used in agriculture, medicine, cosmetic and food industries, and can be used for restoration of pollutant environment together with other organic pollution degrading microbes. The bacterial strain is resistant to a plurality of antibiotics with easy fermentation course and convenient operation.

Description

technical field [0001] The invention belongs to the field of environmental microbial engineering, and in particular relates to a lipopeptide-producing bacillus pumilus and an application thereof. Background technique [0002] Tens of millions of tons of petroleum hydrocarbons enter the environment every year due to oil exploitation, transportation, storage, and accidental leakage, causing serious pollution of soil, groundwater, water systems, and marine environments, which has attracted increasing attention. After petroleum pollutants enter the water body, not only the chemical oxygen demand of the water body increases, but also the oil film on the water surface prevents the oxygen in the air from diffusing into the water, resulting in the anaerobic state of the local water area, causing aquatic animals to die due to lack of oxygen , the photosynthesis of aquatic plants will also be destroyed, causing serious harm to the ecosystem. Oil leakage seeps into the soil. After the...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02B09C1/10C02F3/34C12R1/07C12R1/085C12R1/01A62D101/04A62D101/28C02F101/32C02F101/34
Inventor 沈标冉炜曹娟
Owner NANJING AGRICULTURAL UNIVERSITY
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