Sphingomonas sp. and application in preparation of rhamsan gum

The technology of sphingomonas and rhamnose gum is applied in the field of fermentation engineering, which can solve the problems such as blank research and no industrialized production, and achieve the effects of convenient and simple operation, reduced cost, and extensive culture conditions.

Active Publication Date: 2013-04-03
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, rhamnose gum has not been developed on a large scale. Only Kelco Company of the United States has conducted a small-scale research on it. The research in this field in my country is still blank, and its industrial production has not been seen.

Method used

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  • Sphingomonas sp. and application in preparation of rhamsan gum
  • Sphingomonas sp. and application in preparation of rhamsan gum
  • Sphingomonas sp. and application in preparation of rhamsan gum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Fermentation of Sphingomonas sp.PG-8 in a 500 mL shake flask to produce rhamnose gum.

[0038] Seed medium: glucose 10g / L, beef extract 3g / L, peptone 5g / L, MgSO 4 ·7H 2 O0.5g / L, use NaOH solution to adjust pH6.8.

[0039] Fermentation medium: glucose 40g / L, yeast extract 5g / L, (NH 4 ) 2 SO 4 5g / L, K 2 HPO 4 ·3H 2 O2g / L, MgSO 4 ·7H 2 O0.5g / L, use NaOH solution to adjust pH6.8.

[0040] Sphingomonas Sphingomonas sp.PG-8 (CGMCC NO:6833) is inoculated in the seed culture medium, cultivates 15h under the shaker condition of 30 DEG C, 200r / min, the seed liquid is pressed 5% (v / Seeds of v) were inoculated into shake flasks pre-filled with 500mL sterilized fermentation medium for cultivation. The cultivation conditions were: 30°C, 200r / min, fermentation for 72h, and the rhamnose content reached 20.6g / L.

Embodiment 2

[0041] Example 2: Batch fermentation of Sphingomonas sp.PG-8 in a 3L fermenter to produce rhamnose gum.

[0042] Seed medium and fermentation medium are the same as in Example 1.

[0043] Sphingomonas Sphingomonas sp.PG-8 (CGMCC NO:6833) is inoculated in the seed culture medium, cultivates 15h under the shaker condition of 30 DEG C, 200r / min, the seed liquid is pressed 8% (v / The amount of seeds of v) was inoculated in a fermenter pre-installed with 2.1L sterilized fermentation medium for cultivation. The cultivation conditions: 30°C, 400r / min, ventilation rate 3.0L / min, open the pH automatic during fermentation The control device uses 3mol / L HCl or 10% ammonia water (v / v) to control the pH value of the fermentation broth between 6.5-7.5. After 48 hours of fermentation, the content of rhamnose gum reached 23.5g / L.

Embodiment 3

[0044] Example 3: Batch fermentation of Sphingomonas sp.PG-8 in a 3L fermenter to produce rhamnose gum.

[0045] Seed medium and fermentation medium are the same as in Example 1.

[0046] Sphingomonas Sphingomonas sp.PG-8 (CGMCC NO:6833) was inoculated in the seed culture medium, cultivated for 15h under the shaker condition of 32°C and 200r / min, and the seed liquid was pressed 5% (v / The amount of seeds of v) was inoculated in a fermenter pre-installed with 2.1L of sterilized fermentation medium for cultivation. The cultivation conditions: 30°C, 600r / min, ventilation rate 2.3L / min, open pH automatic during fermentation The control device uses 3mol / L HCl or 3mol / L NaOH solution to control the pH value of the fermentation broth between 7.0-7.5. After 48 hours of fermentation, the content of rhamnose gum reached 28.9g / L.

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Abstract

The invention discloses sphingomonas sp. The sphingomonas sp. is named as sphingomonas sp. PG-8 and was collected in China General Microbiological Culture Collection Center (CGMCC), the registration number is CGMCC NO. 6833, and the collection data is November 15th, 2012. The invention also discloses application of the sphingomonas sp. in preparation of rhamsan gum. The strain is cultured in a culture medium containing carbon sources, nitrogen sources and inorganic salts, and 5 to 50g / L of rhamsan gum can be accumulated under optimized conditions. The strain can be cultured by using multiple carbon sources and nitrogen sources, the culture conditions are extensive, the operation is convenient and simple, the yield of the rhamsan gum is high, the production cost is low, and the rhamsan gum can be widely applied in the fields of food addition, petroleum drilling and the like.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and relates to a high-yielding rhamnose gum microbial strain Sphingomonas sp. and its application. Background technique [0002] Rhamnan Gum (Rhamsan Gum) is an exopolysaccharide produced by Sphingomonas and is a kind of gellan gum polysaccharide. Gellan gum polysaccharides have the same main chain structure of four sugar repeating units: D-glucose, D-glucuronic acid, D-glucose and L-rhamnose, but there are some slight differences in side chains or other structures. The structure of rhamnose gum is shown in (1). With its unique physical and rheological properties and safety in use, rhamnose gum can be used as a suspending agent, stabilizer, thickener, emulsifier, film-forming agent, etc. in food, petroleum, cement, and paint industries. , Also used in ink, food, textile printing and dyeing, pesticides, cosmetics, medicine and other industries. [0003] [0004] At present, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04C12R1/01
Inventor 徐虹徐晓滢董淑浩李莎粱金丰
Owner NANJING UNIV OF TECH
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