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A strain of Bacillus subtilis and its application in fermentative production of adenosine

A technology of Bacillus subtilis and adenosine, which is applied in the field of Bacillus subtilis and its application in the fermentation and production of adenosine, which can solve the problem that adenosine cannot meet the market demand, the fermentation yield and conversion rate are low, and the raw materials and energy consumption are large and other problems, to achieve the effect of good industrial application prospects, high yield and conversion rate, and improved strain stability

Active Publication Date: 2021-06-15
江苏澳创生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, adenosine fermentation strains have high requirements on raw materials, culture conditions and control process, poor strain stability, relatively low fermentation yield and conversion rate, large raw materials and energy consumption, and high production costs, resulting in There are few adenosine manufacturers at home and abroad, and the adenosine produced cannot meet the market demand

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: A strain of Bacillus subtilis and its application in fermentative production of adenosine

[0026] A strain of Bacillus subtilis ACA301, which is classified as Bacillus subtilis, was preserved in the China General Microorganism Culture Collection Center (CGMCC) on November 19, 2018, and the culture preservation number is CGMCC No. .16753.

[0027] The Bacillus subtilis ACA301 and its application in fermentative production of adenosine comprise the following steps:

[0028] (1) Strain culture, inoculate the preserved Bacillus subtilis ACA301 strain in a sterile activation medium, cultivate at 34°C for 21 hours until fresh bacteria grow, sterilize the seed medium, cool to 40°C, The pH was adjusted to 7, and the activated Bacillus subtilis ACA301 strain was inoculated into the seed medium for cultivation until the logarithmic growth phase. Strain culture conditions: temperature 34°C, pH value 7, dissolved oxygen 12%, tank pressure 0.035MPa.

[0029] (2) Ferme...

Embodiment 2

[0038] Example 2: A strain of Bacillus subtilis and its application in fermentative production of adenosine

[0039] The Bacillus subtilis ACA301 and its application in fermentative production of adenosine comprise the following steps:

[0040] (1) Strain cultivation, inoculate the preserved Bacillus subtilis ACA301 strain in a sterile activation medium, cultivate at 28°C for 18 hours until fresh bacteria grow, sterilize the seed medium, cool to 40°C, The pH was adjusted to 6.5, and the activated Bacillus subtilis ACA301 strain was inoculated into the seed medium for cultivation until the logarithmic growth phase. Strain culture conditions: temperature 28°C, pH 6.5, dissolved oxygen 10%, tank pressure 0.02MPa.

[0041] (2) Fermentation culture, the fermentation medium is sterilized, cooled to 40 DEG C, and the pH is adjusted to 6.5, the logarithmic growth phase bacterial classification obtained in step (1) is inserted into the fermentation medium for fermentation and cultivat...

Embodiment 3

[0050] Example 3: A strain of Bacillus subtilis and its application in fermentative production of adenosine

[0051] The mutagenesis screening method of Bacillus subtilis ACA301 described in the present invention is as follows:

[0052] Using Bacillus subtilis CICC10082 as the starting strain, through multiple times of diethyl sulfate (DES) chemical mutagenesis, ultraviolet mutagenesis, and then separation and purification, the adenosine high-yielding strain with substrate resistance and genetic markers was screened.

[0053] Starting bacteria: Bacillus subtilis CICC10082

[0054] Starting bacteria characteristics: G+, irregular bacteria, movement. The colony is round, with smooth surface, low convex surface, notched edge, off-white, translucent. Liquid cultures are cloudy. Gelatin liquefies. V.P positive, M.R. positive, H2S positive, urease negative, catalase positive, aerobic. The optimum growth temperature is 28-32°C, and the maximum growth temperature is 50°C. Litmus ...

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Abstract

A strain of Bacillus subtilis and its application in fermentative production of adenosine, wherein the Bacillus subtilis is Bacillus subtilis ( Bacillus subtilis ) ACA301, the Bacillus subtilis ACA301 is preserved in China General Microorganism Culture Collection Management Center, and the culture preservation number is CGMCC No.16753. The stability of the Bacillus subtilis ACA301 in the present invention is greatly improved, and a breakthrough has been made in technology. The production of adenosine by fermentation of this strain has a relatively high level of yield and conversion rate, and is significantly advanced. , and the fermentation raw materials are easy to obtain, the cultivation conditions are extensive, and the control process is simple, so it is suitable for industrial production and has good industrial application prospects.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a strain of Bacillus subtilis and its application in fermentative production of adenosine. Background technique [0002] Adenosine (Adenosine), chemical name 6-amino-9-β-D-ribofuranosyl-9-hydropurine, adenosine is an important nucleotide derivative, which is the product of adenine nucleotide dephosphorylation. As an endogenous nucleoside throughout human cells, it can directly enter the myocardium to be phosphorylated to generate adenosine, and participate in myocardial energy metabolism. Adenosine plays important roles in biochemistry, including energy transfer in the form of adenosine triphosphate (ATP) or adenosine diphosphate (ADP), or signal transmission in the form of cyclic adenosine monophosphate (cAMP). In addition, adenosine is an inhibitory neurotransmitter and plays an important role in neurotransmission. Adenosine is widely used in medicine and other industries...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P19/40C12R1/125
CPCC12N1/20C12P19/40C12N1/205C12R2001/125
Inventor 王健周旭波方培新王华萱
Owner 江苏澳创生物科技有限公司
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