Epicoccum nigrum DB3 bacterial strain as well as preparation and application thereof
The technology of E. nigricans and strains is applied in the directions of biochemical equipment and methods, fungi, and chemical methods to produce bast fibers, which can solve the problems of high energy consumption in chemical treatment processes, inability to recycle waste water, and large equipment loss, etc. Achieve the effect of being suitable for large-scale industrial production, low processing cost, and strong anti-pollution ability
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Embodiment 1
[0041] Screening and Obtaining of Flax Degumming Strains
[0042] (1) Sampling rotting seaweed from the Zhoushan sea area of the East China Sea, first put the seaweed and the enrichment medium at a ratio of 1g: 20ml in the enrichment medium and culture it at room temperature for 30 days, then take 0.1ml of the enrichment medium and apply it to the separation medium Culture medium, static culture at 30°C for 1 to 4 days, to obtain the dominant strain of wild-type flax biotreatment;
[0043] Among them, the formula of the enrichment medium is: 5 g of flax powder, 200 ml of tap water, and the pH is natural; the formula of the separation medium is: 5 g of flax powder, (NH 4 ) 2 SO 4 5g, K 2 HPO 4 1g, MgSO 4 0.5g, KCl 0.5g, FeSO 4 0.01, 20g agar, 1000mL water, adjust the pH to 7-7.2 with NaOH;
[0044] (2) Inoculate the bacterial strain obtained in the step (1) into flax lignin nutrient medium, and culture at 28° C. for 72 hours. Flax lignin nutrient medium, its componen...
Embodiment 2
[0047] Preparation of fermentation broth:
[0048] (1) Alternaria DB2 strains were stored in potato dextrose agar medium, cultured at 30°C and 220rpm for 48 hours, and added freezing buffer; freezing buffer consisted of potassium dihydrogen phosphate 0.0627g, dipotassium hydrogen phosphate 0.0177g, sodium citrate 0.0588g, 0.02645g of magnesium sulfate heptahydrate, 10ml of glycerin, add water to make up to 100ml;
[0049] (2) In the sterilized and cooled 50mL potato dextrose medium, insert the bacteria ring obtained in step (1), and shake the flask for 48 hours at a rotation speed of 220r / min and a temperature of 30°C;
[0050] (3) Inoculate the culture medium obtained in step (2) into 5 L of flax lignin fermentation medium, and shake the flask for 48 hours at a rotation speed of 220 r / min and a temperature of 30° C. to obtain a fermentation broth; wherein, the formulation of the flax fermentation medium is: : Flax raw hemp lignin 5g, KCl 0.05g, dipotassium hydrogen phosphate...
Embodiment 3
[0052] The flax degumming process is as follows:
[0053] Put 5g of the original stem of flax into a 250ml conical flask, put the original flax stem and the fermented bacterium liquid of Example 2 into the conical flask according to the bath ratio of 1:20, the liquid volume is 100ml, at 40°C, in a shaker at 200rpm Respectively degumming for 1 day; when the degumming time is up, remove the degumming solution and stop the biological treatment. The biologically treated raw flax stems are inactivated, and the process is as follows: the biologically treated flax raw stems are mixed with the degumming solution at a ratio of 1:10, and treated at 70°C for 3 hours, wherein the peroxide The dosage is 3% of the original flax stem, the dosage of urea is 0.05% of the original flax stem, the dosage of sodium tripolyphosphate is 2% of the original flax stem, and the dosage of disodium edetate is 0.1% of the original flax stem . After degumming, 2130 flax fibers for textile can be produced....
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