Sphingomonas sp.815Y strain for degrading bromate and application of Sphingomonas sp.815Y strain

A sphingomonas and bromate technology, applied in the field of sphingomonas strains and microbial strains, can solve the problems of less progress of bromate, complicated process operation, secondary pollution, etc. Low maintenance cost, reduced carcinogenic toxicity, and fast degradation

Active Publication Date: 2013-05-15
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the bromate removal rate of this method can reach more than 60%, the treatment method needs to control dissolved oxygen, which is difficult to apply in the actual process, and the process operation is complicated, which is easy to cause secondary pollution
[0005] The work on the biodegradation of bromate has little progress at home and abroad

Method used

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  • Sphingomonas sp.815Y strain for degrading bromate and application of Sphingomonas sp.815Y strain
  • Sphingomonas sp.815Y strain for degrading bromate and application of Sphingomonas sp.815Y strain
  • Sphingomonas sp.815Y strain for degrading bromate and application of Sphingomonas sp.815Y strain

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Isolation and purification of embodiment 1 bacterial strain

[0054] 1. Culture medium preparation

[0055] (1) Enrichment medium:

[0056] CaCl 2 5mg, MgCl 2 5 mg, MnCl 2 0.3mg, FeCl 2 0.3mg, ZnCl 2 0.01 mg, H 3 BO 3 0.01mg, CoCl 2 0.005mg, bromate 1mg, sodium acetate 10mg, distilled water 1,000mL, pH 7.0-8.0, and the culture medium was sterilized at 121°C for 20 minutes.

[0057] (2) Separation and purification medium:

[0058] Yeast extract 0.5g, tryptone 0.5g, acid hydrolyzed casein 0.5g, glucose 0.5g, soluble starch 0.5g, K 2 HPO 4 0.3g, MgSO 4 ·7H 2 O 0.05g, sodium pyruvate 0.3g, agar 15g, distilled water 1,000mL, pH 7.0-8.0, and the medium was sterilized at 121°C for 20 minutes.

[0059] (3) LB solid medium

[0060] Tryptone 10g, yeast extract 5g, NaCl 10g, agar 15g, distilled water 1,000mL, pH7.2. The medium was steam sterilized at 15 psi for 20 minutes before use.

[0061] 2. Phosphate buffer

[0062] Phosphate buffer is a mixed solutio...

Embodiment 2

[0069] Research on the microbiological characteristics of embodiment 2 Sphingomonas bacterial strains

[0070] 1. Colony morphology observation

[0071] Streak-inoculate the isolated and purified single-cell strains on LB solid medium, culture at 35°C until colonies grow, and observe the colony morphology. The results are as follows:

[0072] The colony characteristics of the strain are: the diameter of the colony cultivated on the LB plate for 2 days is about 1-2mm, the colony is yellow, moist, drop-shaped, round, raised, opaque, and the edges are neat. Such as figure 1 shown.

[0073] 2. Observation of cell morphology

[0074] The colonies of the strains cultured on LB plates for 2 days were picked, fixed with glutaraldehyde, rinsed with phosphate buffer (pH7.2), dehydrated with gradient ethanol, treated with isoamyl acetate, dried at the critical point of carbon dioxide, sprayed with gold, and After the sample was placed in the observation room, it was scanned by an ele...

Embodiment 3

[0082] 1) Preparation of wet cells of single cell strain Sphingomonas sp.815Y

[0083] Use an inoculation loop to take fresh slant strains of Sphingomonas sp. 815Y, streak and inoculate them on LB solid medium, and cultivate them at 35°C for 2 days to obtain a medium plate with obvious colonies. Obtain fresh Sphingomonas wet cells.

[0084] 2) Bromate removal test

[0085] Pick the wet cells of the strain Sphingomonas sp.815Y and inoculate them in the enriched medium, and cultivate them at 25°C under dark conditions, wherein 0.2g of wet cells are inoculated in every 50mL of the enriched medium; the bromine in the culture medium Salt and bromide ion content adopts ion chromatography to measure according to the method of national standard GB / T 5750.10-2006 (drinking water standard test method disinfection by-product index), and measure the culture medium before inoculation and the 9th day, 15th day after inoculation, The concentration value of bromate and bromide ion in the cu...

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Abstract

The invention discloses a Sphingomonas sp.815Y strain for degrading bromate and application of the Sphingomonas sp.815Y strain, wherein the microbial preservation number of the Sphingomonas sp.815Y strain is CGMCC (China General Microbiological Culture Collection Center) No.4721. The Sphingomonas sp.815Y strain is obtained by screening from the natural world, can be used for effectively degrading the bromate, is high in degrading efficiency, can be inoculated and applied to a biological activated carbon process, has the capabilities of effectively removing the bromate produced in an ozonization process of drinking water and the bromate in polluted drinking water and improving the safety of the drinking water and has a favorable application prospect in terms of drinking water treatment.

Description

technical field [0001] The invention relates to a microbial strain for removing harmful pollutants, in particular to a strain of Sphingomonas sp. for degrading bromate, and belongs to the field of microbial degradation of bromate. Background technique [0002] With the development of water treatment technology and people's attention to the quality of drinking water, the application of ozone oxidation technology in drinking water is becoming more and more extensive. Ozone has an obvious inactivation effect on most bacteria, viruses, fungi, protozoa, and oocysts. At the same time, it can efficiently remove various trace harmful pollutants such as disinfection by-product precursors and odor substances in water. However, some by-products produced in the ozonation process also have a certain negative impact on the safety of drinking water. For example, bromate will be produced during the ozonation process of bromine-containing raw water, which is carcinogenic and difficult to be ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C02F3/34C12R1/01
Inventor 杨敏刘娟于建伟王永京
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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