Fluoroglycofen degrading bacteria and bacterial agent prepared from same
A pesticide residue degradation and bacterial strain technology, which is applied in the field of high biotechnology, can solve the problems of residues, achieve low production and use costs, good removal effect, and protect the ecological environment
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[0020] 1. Shake flask degradation experiment
[0021] In the inorganic salt medium containing 100mg / L acifluorfen (the formulation of the inorganic salt medium is: every liter contains 1.5gK 2 HPO 4 , 0.5g KH 2 PO 4 , 0.2g MgSO 4 ·7H 2 O, 1.0g NaCl, 1.0g (NH 4 ) 2 SO 4 , pH 7.0), MBLHY-1 was inoculated at an inoculum size of 5%, cultured with shaking at 30°C, and samples were taken for measurement after 72 hours. Compared with the blank, the absorption peak of the treated sample decreased obviously, and acifluorfen was degraded by more than 90%.
[0022] 2. Soil degradation experiment
[0023] The first group: Take 60g of naturally air-dried vegetable field soil after sieving (2mm), add acifluorfen to 100mg / kg, stir evenly, and spray water to make the water content reach 40% of the saturated water content. Then divide evenly into 6 plastic cups, 10g per cup. Add the centrifuged sterile water resuspension solution (OD 600nm ≈2.0) 1ml, stir evenly; add 1ml of sterile...
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