Fluoroglycofen degrading bacteria and bacterial agent prepared from same

A pesticide residue degradation and bacterial strain technology, which is applied in the field of high biotechnology, can solve the problems of residues, achieve low production and use costs, good removal effect, and protect the ecological environment

Active Publication Date: 2010-12-29
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extensive use of fluorine-containing pesticides leads to the residue of such pesticides in the soil and water environment, which may pose a potential threat to the ecological environment and human health. Therefore, environmental behaviors such as the migration, transformation and decomposition of fluorine-containing pesticides in the environment are increasingly being focus on

Method used

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  • Fluoroglycofen degrading bacteria and bacterial agent prepared from same
  • Fluoroglycofen degrading bacteria and bacterial agent prepared from same
  • Fluoroglycofen degrading bacteria and bacterial agent prepared from same

Examples

Experimental program
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Effect test

Embodiment Construction

[0020] 1. Shake flask degradation experiment

[0021] In the inorganic salt medium containing 100mg / L acifluorfen (the formulation of the inorganic salt medium is: every liter contains 1.5gK 2 HPO 4 , 0.5g KH 2 PO 4 , 0.2g MgSO 4 ·7H 2 O, 1.0g NaCl, 1.0g (NH 4 ) 2 SO 4 , pH 7.0), MBLHY-1 was inoculated at an inoculum size of 5%, cultured with shaking at 30°C, and samples were taken for measurement after 72 hours. Compared with the blank, the absorption peak of the treated sample decreased obviously, and acifluorfen was degraded by more than 90%.

[0022] 2. Soil degradation experiment

[0023] The first group: Take 60g of naturally air-dried vegetable field soil after sieving (2mm), add acifluorfen to 100mg / kg, stir evenly, and spray water to make the water content reach 40% of the saturated water content. Then divide evenly into 6 plastic cups, 10g per cup. Add the centrifuged sterile water resuspension solution (OD 600nm ≈2.0) 1ml, stir evenly; add 1ml of sterile...

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Abstract

The invention provides a degrading bacterial agent for removing fluoroglycofen residue, and belongs to the field of biological high technology. The strain is gram-negative bacteria MBLHY-1 which are found to be sphingomonas sp. through identification; and biologically, the strain is gram-negative, rodlike, sporeless and 0.8-1.3*0.5-0.6mu m long with polarflagella, is catalase-positive, oxidase-negative, voges-proskauer reaction-positive and methyl red reaction-negative, cannot hydrolyze starch or liquefy gelatin, does not generate indole, and can hydrolyze Tween-80. The pesticide residue in soil is reduced by over 90 percent and the problem that the pesticide residue exceeds the standard in agricultural production is solved by directly applying a degrading bacteria product; and nontoxic and pollution-free agricultural products can be produced.

Description

1. Technical field [0001] The invention discloses a fluorinated acetoxyfen residue-degrading bacterium and the bacterium agent produced therefrom, belonging to the field of biological high technology, degrading chemical pesticides by means of microorganisms, and applicable to the production and processing of green and pollution-free agricultural products in modern agricultural production. 2. Background technology [0002] Fluorine-containing pesticides are emerging pesticides that have been used in recent decades. The fluorine atom has the characteristics of small radius and high electronegativity. The introduction of fluorine atoms and fluorine-containing groups (such as: -CF3) into biologically active organic compounds can greatly improve the stability and physiological activity of organic fluorides. So it has become a hot spot of research and development today. However, the extensive use of fluorine-containing pesticides leads to the residues of such pesticides in the so...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/04A62D101/28
Inventor 蔡天明陈立伟钱丽花顾海莎
Owner NANJING AGRICULTURAL UNIVERSITY
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