Sphingomonas sp. and method for preparing diutan gum fermentation liquid by using same

A technology of sphingomonas and fermentation liquid, which is applied in the field of sphingomonas and its use to prepare the fermented liquid of dextrose gum, which can solve the problems of unreasonable production cost and low yield, and achieve good sphingomonas The effect of high-quality rubber production capacity

Active Publication Date: 2015-01-21
张星昊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional production process has the shortcomings of high production cost and low yield (generally about 15-25g / L) due to unreasonable process design (such as culture medium, etc.)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Utilize sphingomonas to prepare the method for diyou gum fermentation broth, the method comprises the following process steps:

[0080] A. Inoculate Sphingomonas sp. (Sphingomonas sp.) CGMCCNo.9531 into a sterile culture solution containing carbon source, nitrogen source and necessary nutrients;

[0081] B, adjust the pH of the nutrient solution, and carry out aeration and stirring fermentation;

[0082] C. When the viscosity of the culture medium no longer increases or the fermentation period is less than or equal to 65 hours, the fermentation ends.

[0083] The carbon source and nitrogen source in the culture solution are selected from waste molasses, corn hydrolyzate and soybean meal hydrolyzate.

[0084] The corn hydrolyzate is produced by the following method: crush the corn to 40-60 mesh, use tap water to prepare a mixed solution with a concentration of 30% by mass, adjust the pH to 8-9, raise the temperature to 75-85°C, and maintain the temperature at 60°C. Min...

Embodiment 2

[0115] The difference between this embodiment and embodiment 1 is:

[0116] The culture solution is composed of the following components in mass percentage:

[0117] Waste molasses 4.5%; corn hydrolyzate 1.5%; soybean meal hydrolyzate 0.5%; dipotassium hydrogen phosphate 0.25%; polyether defoamer 0.15%; the balance is sterile water; pH=8.0-8.5.

[0118] The shake flask seed medium is composed of the following components in mass percentage:

[0119] White sugar 2.0%; Tryptone 0.9%; Thymine 1ppm; The balance is sterile water pH=8.0-9.0.

[0120] The first-level seed culture solution is composed of the following components in mass percentage:

[0121] White sugar 3%; tryptone 0.3%; soybean powder 0.3%; the rest is sterile water; pH=8.0-9.0;

[0122] The amount of inoculum is the shake flask seeds: primary seed culture solution=0.8%-1.0%. The shake flask seeds are inserted into the sterilized primary seed culture solution and made into primary seeds through cultivation. During ...

Embodiment 3

[0127] The difference between this embodiment and embodiment 1 is:

[0128] The culture solution is composed of the following components in mass percentage:

[0129] Waste molasses 4.2%; corn hydrolyzate 1.5%; soybean meal hydrolyzate 0.4%; dipotassium hydrogen phosphate 0.22%; polyether defoamer 0.1%; the balance is sterile water; pH=8.0-8.5.

[0130] The shake flask seed medium is composed of the following components in mass percentage:

[0131] White sugar 1.5%; Tryptone 0.7%; Thymine 1ppm; The balance is sterile water pH=8.0-9.0.

[0132] The first-level seed culture solution is composed of the following components in mass percentage:

[0133] White sugar 2.5%; tryptone 0.25%; soybean powder 0.25%; the rest is sterile water; pH=8.0-9.0;

[0134] The amount of inoculum is the shake flask seeds: primary seed culture solution=0.8%-1.0%. The shake flask seeds are inserted into the sterilized primary seed culture solution and made into primary seeds through cultivation. Duri...

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PUM

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Abstract

The invention belongs to the preparation of microbial polysaccharides, and in particular relates to sphingomonas sp. and a method for preparing a diutan gum fermentation liquid by using the same. The method comprises the following steps: inoculating sphingomonas sp. with the collection number of CGMCC No.9531 into a sterilized culture solution containing a carbon source, a nitrogen source and necessary nutrient substances; adjusting the pH value of the culture solution, and performing aeration stirring fermentation; and performing process steps such as fermentation ending when the viscosity of the culture solution does not increase any more or the fermentation cycle of the culture solution is less than or equal to 65 hours. Sphingomonas sp. and the method for preparing the diutan gum fermentation liquid by using sphingomonas sp. disclosed by the invention can be used for solving the technical problems of high production cost, low yield and the like caused by unreasonable process design in the prior art, and have the advantages of high yield, good temperature resistance of products, low fermentation cost and the like.

Description

technical field [0001] The invention belongs to microbial fermentation, in particular to a sphingomonas and a method for preparing the fermented gum fermented liquid by using it. Background technique [0002] Diutan gum is a microbial polysaccharide with excellent performance, which is a water-soluble biopolymer produced by microbial fermentation. It has excellent thickening properties, pseudoplasticity, high temperature resistance, high salt and a wide range of pH adaptability. It can be widely used in petroleum, cement and other industries, and has broad application prospects. [0003] The traditional diutan gum production process is that the production bacteria use glucose as raw material for aerobic fermentation. During the fermentation, the medium with glucose, nitrate, inorganic salt, etc. as the main raw materials is prepared in proportion, and after sterilization, it is inoculated with Diyou gum production strains, and the fermentation is carried out at a certain te...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04C12R1/01
CPCC12P19/04C12N1/205C12R2001/01
Inventor 张星昊孙文哲
Owner 张星昊
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