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Fermentation process for production of welan gum via Sphingomonas sp.WG

A technology of sphingomonas and fermentation technology, which is applied in the field of high-yield welan gum fermentation technology, can solve the problems of limiting the fermentation yield of welan gum, and the lack of understanding of optimal culture conditions and nutritional conditions.

Inactive Publication Date: 2017-06-30
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of understanding of the optimal culture conditions and nutritional conditions required by the strain, the further improvement of the fermentation yield of welan gum is limited.

Method used

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  • Fermentation process for production of welan gum via Sphingomonas sp.WG
  • Fermentation process for production of welan gum via Sphingomonas sp.WG
  • Fermentation process for production of welan gum via Sphingomonas sp.WG

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Experimental program
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Effect test

Embodiment 1

[0022] Initial fermentation medium composition (g / L): glucose 40, yeast powder 2, dipotassium hydrogen phosphate 4, potassium dihydrogen phosphate 2, magnesium sulfate 0.1, deionized water to volume.

[0023] The initial culture conditions are as follows: the inoculum size is 5% (v / v), the filling volume is 50mL (250mL Erlenmeyer flask), the medium pH is natural pH, the culture temperature is 28°C, the shaking table speed is 150rpm, and the fermentation cycle is 72h.

Embodiment 2

[0025] The culture conditions (such as inoculum size, liquid volume, initial pH of medium, temperature, rotation speed) that may affect the yield of welan gum were optimized by single factor experimental design. It is specifically set as: inoculum size 1%, 3%, 5%, 6%, 7%, 8%, 10% (v / v); , 150mL, the culture temperature is 25, 27.5, 30, 32.5, 35°C, the initial pH of the medium is 2-10, and the shaker speed is 125, 150, 175, 200, 225rpm. The culture medium is the initial fermentation medium.

[0026] Detect the yield of welan gum under different fermentation conditions, the results are as follows: figure 1 As shown, the following conclusions can be drawn: 1. With the increase of inoculation amount, the yield of welan gum showed a trend of rising first and then decreasing, and the optimal inoculation amount was 5%. 2. When the liquid volume is 50mL, it is most beneficial to the production of Welan gum. 3. The initial pH of the medium had a great influence on the production of ...

Embodiment 3

[0028] In the initial fermentation medium, 4% (w / v) of different sugar substances were used to replace glucose as carbon source: fructose, mannitol, L-sorbose, D-xylose, D-arabinose, glucose, D - Galactose, maltose, lactose, cellobiose, raffinose, dextran (T5), soluble starch and cellulose. The selected sugars replaced glucose as the single carbon source, and the culture conditions were optimal fermentation conditions. After 72 hours of fermentation, the yield of welan gum in the fermentation broth was measured.

[0029] For the production of welan gum corresponding to different carbon sources, see figure 2 . It can be seen from the figure that when the carbon source is glucose, the yield of welan gum is the highest, so the single factor design is used to optimize the concentration of glucose, the selected glucose concentration is 10-80g / L, and the yield when the glucose concentration is 60g / L Highest.

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Abstract

The invention relates to a fermentation process for production of welan gum via Sphingomonas sp.WG, specifically to a high-yield fermentation production process for welan gum, belonging to the technical field of fermentation engineering. The fermentation process for production of welan gum via Sphingomonas sp.WG is characterized in that a fermentation medium is composed of (g / L) 72.4 of glucose, 3.58 of yeast powder, 3 of K<2>HPO<4>, 0.1 of MgSO<4> and 0.1 of ZnSO<4> and has a pH value of 7.0; and fermentation conditions comprise a culture volume of 50 mL (in a 250-mL Erlenmeyer flask), an inoculation amount of 2.5 ml (5%, v / v), an initial pH value of the medium of 7, a culture temperature of 32.5 DEG C, a shaking-table rotating speed of 200 rpm and fermentation time of 72 h, and fermentation is accomplished under the fermentation conditions. Under the above-mentioned fermentation conditions, the output of welan gum reaches 40.47 g / L, being 2.53 times of the output before optimization (15.98 g / L), and the output reaches a leading level in the world. The fermentation production process for the welan gum is low in cost, simple to operate, high in efficiency, easy to promote and beneficial for realizing large-scale industrial production of the welan gum, and has high economic benefits and good application prospects.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a high-yield welan gum fermentation process. Background technique [0002] Welan gum (welan gum) is a polysaccharide polymer synthesized by microorganisms. The main chain structure consists of four sugar repeating units (D-glucose-D-glucuronic acid-D-glucose-L-rhamna Sugar), the side chain is composed of L-mannosyl or L-rhamnosyl. [0003] Welan gum has good stability, suspending, emulsifying, thickening and unique shear thinning properties, so it can be used as thickener, suspending agent, emulsifier, stabilizer, lubricant, film-forming agent And adhesives are used in various aspects of industry and agriculture, especially in pharmaceuticals, food, chemicals, graphite, concrete and biological oil recovery. [0004] At present, welan gum is mainly produced through microbial fermentation. The American kelco company is now the world's largest producer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04C12R1/01
CPCC12P19/04C12R2001/01C12N1/205
Inventor 李慧朱虎周万龙孙世伟孙亚杰焦学李菁
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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