Method for synthesizing Welan gum with specific viscosity

A technology of welan gum and viscosity, which is applied in the field of synthesizing welan gum with specific viscosity, can solve the problems such as inability to produce welan gum, and achieve the effect of simple and easy method.

Inactive Publication Date: 2017-03-29
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the production methods of welan gum are mainly focused on increasing the output of welan gum, and it is not possible to produce welan gum with a specific viscosity according to the needs.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1 production viscosity is at the welan gum of 1.5~1.9Pas

[0021] Get 2 loops of the strains activated on the inclined plane, inoculate them into 500mL seed bottle, the liquid volume is 100mL, 200r / min, 30 ℃ constant temperature shaker culture for 24h to obtain seed liquid, inoculate to 7L fermenter ( Bioflo115 fermenter, New Brunswick Company, USA), ventilation rate 1vvm, rotating speed correlated with dissolved oxygen, controlled dissolved oxygen 30%, fermentation cycle 70h, liquid volume 4L, pH value controlled at 7.0. The composition of the fermentation medium is calculated in g / L: 40 sucrose, 2.5 beef extract, 0.5 sodium nitrate, KH 2 PO 4 0.6, MgSO 4 ·7H 2 O 0.2, pH 7.0.

Embodiment 2

[0022] Embodiment 2 production viscosity is at the welan gum of 1.9~2.7Pas

[0023] Get 2 loops of the strains activated on the inclined plane, inoculate them into 500mL seed bottle, the liquid volume is 100mL, 200r / min, 30 ℃ constant temperature shaker culture for 24h to obtain seed liquid, inoculate to 7L fermenter ( Bioflo115 fermenter, New Brunswick Company, USA), ventilation rate 1vvm, rotating speed correlated with dissolved oxygen, controlled dissolved oxygen 35%, fermentation cycle 70h, liquid volume 4L, pH value controlled at 7.0. The composition of the fermentation medium is calculated in g / L: sucrose 40, beef extract 1.5, sodium nitrate 2.0, KH 2 PO 4 0.6, MgSO 4 ·7H 2 O 0.2, pH 7.0.

Embodiment 3

[0024] Embodiment 3 production viscosity is at the welan gum of 2.7~3.8Pas

[0025] Get 2 loops of the strains activated on the inclined plane, inoculate them into 500mL seed bottle, the liquid volume is 100mL, 200r / min, 30 ℃ constant temperature shaker culture for 24h to obtain seed liquid, inoculate to 7L fermenter ( Bioflo115 fermenter, New Brunswick Company, USA), ventilation rate 1vvm, rotating speed correlated with dissolved oxygen, controlled dissolved oxygen 40%, fermentation cycle 70h, liquid filling volume 4L, pH value controlled at 7.0. The composition of the fermentation medium is calculated in g / L: 40 sucrose, 1 beef extract, 4 sodium nitrate, KH 2 PO 4 0.6, MgSO 4 ·7H 2 O 0.2, pH 7.0.

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Abstract

The invention discloses a method for synthesizing Welan gum with specific viscosity and belongs to the field of biochemical engineering. The Welan gum is synthesized by sphingomonas sp. fermentation. The production of the Welan gum with the viscosity scope being 1.5-3.8 Pas is implemented by controlling the condition that a fermentation medium is composed of 40 g / L of sucrose, 0-4 g / L of beef extract, 0-4 g / L of sodium nitrate, 0.6 g / L of KH2PO4 and 0.2 g / L of MgSO4.7H2O at the pH value of 7.0. The method for synthesizing the Welan gum with the specific viscosity is simple and practicable.

Description

technical field [0001] The invention relates to a method for synthesizing specific viscosity welan gum, which belongs to the field of biochemical engineering. Background technique [0002] Welan gum is a microbial exopolysaccharide fermented by Gram-negative coryneform bacteria (Sphingomonas sp.). The structural skeleton of welan gum consists of D-glucose, D-glucuronic acid, D-glucose and Composed of L-rhamnose units. C in glucose and rhamnose-linked glucose residues 3 There are α-L-rhamnose or α-L-mannose branched chains connected to the position, and the probability of connecting rhamnose accounts for 2 / 3; in addition, about half of the tetrasaccharide fragments have acetyl and glycerol groups . Welan gum contains 2.8%-7.5% acetyl groups, 11.6%-14.9% glucuronic acid, and the molar ratio of mannose, glucose and rhamnose is 1:2:2. [0003] Welan gum is a new type of microbial polysaccharide, which has been widely used in many industrial fields as thickener, emulsifier, s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/01
CPCC12P19/04
Inventor 詹晓北许晓鹏郑志永朱莉黄艳艳
Owner JIANGNAN UNIV
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