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Bacterium for degrading chlorpyrifos pesticide residue and produced bacterium formulation

A technology of chlorpyrifos pesticide and degrading bacteria, which is applied in the field of high biotechnology, can solve the problems of narrow degradation spectrum of bacterial strains, use restrictions, etc., and achieve the effects of low production and use costs, protection of the ecological environment, and good removal effect

Inactive Publication Date: 2006-07-12
NANJING AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

In the prior art, our research group already has an invention patent for degrading bacteria (ZL96106317.3, publication number: CN1145408A, publication date: March 19, 1997), which is a strain of Bacillus J5, which can effectively degrade methylamine Phosphorus, but the strain has a narrow degradation spectrum and can only degrade one pesticide, methamidophos, so its use is limited

Method used

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Examples

Experimental program
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Embodiment Construction

[0014] Examples of the present invention are described below.

[0015] In the liquid medium with 100mg / L of the following pesticides as the only carbon source, inoculate 5% of the Dsp-2 grown to the logarithmic phase, and after 24 hours on a shaker, the degradation of the pesticides was detected by high-performance liquid chromatography. The results See the table below.

[0016] substrate

methyl parathion

Profenofos

parathion

Chlorpyrifos

Degradation rate%

100

95.0

100

99.3

[0017] The original seed of the microbial organophosphorus pesticide residue-degrading bacteria Dsp-2 of the present invention is activated on a petri dish, and its degradation performance is measured, and it is inoculated on the inclined surface of a test tube for later use. The test tube seeds were inoculated into 1000ml shake flasks containing 200ml broth medium, cultured with constant temperature shaking until logarithmic phase, and prep...

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Abstract

The invention provides a prodegradant which can dispel the organic phosphorus pesticide residue of chlorpyrifos. The used strain is Gram's stain reaction hysteroptosis Dsp-2 which is Sphingomonas sp. The main biology characteristic is G-; the thaliana is the rod type of asporous whose size is 1.0-1.5um; the scavenger enzyme is male and the oxidative enzyme is female which can not be hydrolysed starched and be gelatin liquidated; the methyl red reaction is female and not to produce the indole with the aquolysis temperature 80 deg. The Genbank landing number of the strain 16S rDNA is AY994060.

Description

1. Technical field [0001] The invention discloses a chlorpyrifos pesticide residue degrading bacterium and a bacterial agent produced therefrom, which belong to the high-tech field of biology, degrade chemical pesticide residues by means of microorganisms, and are suitable for the production and processing of green and pollution-free agricultural products in modern agricultural production. 2. Technical Background [0002] The use of chemical pesticides has greatly promoted the development of modern agriculture, restored a large number of losses caused by diseases and insect pests for agricultural production, and greatly improved the labor efficiency and mechanization degree of agricultural production, playing a role in the modernization of agricultural production. immeasurable effect. But at the same time, pesticides are some toxic and harmful chemicals. After they enter the ecological environment, they not only kill the organisms that are harmful to humans, but also cause s...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/00
Inventor 李顺鹏李晓慧何健洪青贾开志崔中利
Owner NANJING AGRICULTURAL UNIVERSITY
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