Induced sphingomonas paucimobilis as well as preparation method and application of induced sphingomonas paucimobilis

A technology of monosporus and sphingolipids, which is applied in the field of cold gellan gum preparation, can solve the problems of restricting gellan gum production, application and development, difficult to improve product indicators, and lower gel yield, and achieve high viscosity, high gel yield, and high gel yield. The effect of high mutagenicity

Active Publication Date: 2015-05-13
INNER MONGOLIA RAINBOW BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some problems in the large-scale industrial production of gellan gum at present. In the production of gellan gum, with the increase of the number of passages of the gel-producing strains, the bacterial strains will produce extremely obvious degradation, resulting in a high yield of gellan gum. reduce, and the final product index is also difficult to improve, which seriously limits the production application and development of gellan gum; therefore, it is a technical problem that people need to solve urgently to find an improved gel-producing strain to improve the gel yield and the gel index

Method used

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  • Induced sphingomonas paucimobilis as well as preparation method and application of induced sphingomonas paucimobilis

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Experimental program
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preparation example Construction

[0032] Please refer to figure 1 , the preparation method of this mutagenic Sphingomonas pauciformis provided by the invention comprises the following steps:

[0033] Step 101: Inoculate the activated Sphingomonas Paucimobilis strain into a liquid medium for shaking culture to obtain cultured bacterial liquid;

[0034] After activation, Sphingomonas pauciformis is inoculated into a liquid medium for cultivation to ensure that the bacteria can proliferate in large quantities and prepare for subsequent mutagenesis.

[0035] Step 102: centrifuging the cultured bacterial liquid to obtain bacterial cells; and preparing bacterial suspensions from the bacterial cells with an equal volume of physiological saline;

[0036] Using the bacterial liquid cultured in the liquid medium, the bacterial cells are collected by centrifugation, and then the bacterial cells are made into a bacterial suspension with an equal volume of normal saline. Bacteria cells rupture, so that the bacteria from ...

Embodiment 1

[0050] S11: Transfer the Sphingomonas Paucimobilis strain to the activated slant and culture it at a constant temperature of 30°C for 24 hours, then transfer it to a liquid medium, and cultivate it at 30°C at a speed of 200 rpm for 18 hours , to obtain the cultured bacterial liquid;

[0051] S12: Centrifuge the cultured bacterial solution at 4000 rpm for 20 minutes to obtain bacterial cells; add an equal volume of physiological saline to the bacterial cells to prepare a bacterial suspension;

[0052] S13: irradiating the bacterial suspension with a 15-watt ultraviolet lamp 20 cm away from the bacterial suspension for 60 seconds to obtain a mutagenic bacterial liquid;

[0053] S14: adding the primary mutagenizing bacteria solution into a DES solution with a volume fraction of 2%, and incubating on a shaking table for 40 minutes, adding sodium thiosulfate solution to terminate the reaction, and obtaining a secondary mutagenizing bacteria solution;

[0054] Wherein, in this step...

Embodiment 2

[0057] S21: Transfer the Sphingomonas Paucimobilis strain to the activated slant and culture it at a constant temperature of 30°C for 24 hours, then transfer it to a liquid medium, and cultivate it at 30°C at a speed of 200 rpm for 18 hours , to obtain the cultured bacterial liquid;

[0058] S22: Centrifuge the cultured bacterial solution at 4000 rpm for 20 minutes to obtain bacterial cells; add an equal volume of physiological saline to the bacterial cells to prepare a bacterial suspension;

[0059] S23: irradiating the bacterial suspension with a 15-watt ultraviolet lamp 25 cm away from the bacterial suspension for 70 seconds to obtain a mutagenic bacterial liquid;

[0060] S24: adding the primary mutagenizing bacteria solution into a DES solution with a volume fraction of 2%, and incubating on a shaking table for 40 minutes, adding sodium thiosulfate solution to terminate the reaction, and obtaining a secondary mutagenizing bacteria solution;

[0061] Wherein, in this step...

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Abstract

The invention provides an induced sphingomonas paucimobilis and belongs to the field of gellan gum preparation. The induced sphingomonas paucimobilis is collected in China General Microbiological Culture Collection Center, which is located in Institute of Microbiology of Chinese Academy of Sciences, 3#, No.1 Yuan, Western Beichen Road, Chaoyang District, Beijing, with the collection number of CGMCC No.10341 on January 12, 2015. The induced sphingomonas paucimobilis having passage stability can be obtained by inducing the sphingomonas paucimobilis as a starting bacterial strain twice. The induced sphingomonas paucimobilis has passage stability; after the induced sphingomonas paucimobilis is fermented and cultured, the gum-yielding rate of the induced sphingomonas paucimobilis can be 16.25g/L, which is increased by more than 25% in contrast with the gum-yielding rate of the non-induced sphingomonas paucimobilis.

Description

technical field [0001] The invention relates to the field of cold gel preparation, in particular to a mutagenic Sphingomonas paucikinis and its preparation method and application. Background technique [0002] As one of the most superior microbial polysaccharides in the world, gellan gum has excellent quality that animal and plant polysaccharides do not have. It is safe and non-toxic, has unique physical and chemical properties, short production cycle, and is not restricted by climate and geographical conditions. Widely used in more than 20 industries such as food, medicine, chemical industry, agriculture, ceramics, petroleum, etc. [0003] In the food industry, gellan gum can be used as an excellent gelling agent, and the formed gel has high transparency, moderate crispness, and good flavor release. In addition, gellan gum has good compatibility with other food gums, and different food quality requirements can be achieved by adjusting the mixing ratio. In addition, gellan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/01C12N13/00C12P19/04C12R1/01
CPCC12N13/00C12P19/04C12N1/205C12R2001/01
Inventor 古立谦
Owner INNER MONGOLIA RAINBOW BIOTECH CO LTD
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