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Sphingobium yanoikuyae with function of degrading triphenyl phosphate

A technology of ammonia-alcoholic bacteria and bacterial agents, which is applied in the field of microorganisms and biodegradation, can solve the problems of few researches on biodegradation and soil remediation, and achieve the effects of rapid degradation, good economic value and application prospects, and strong salt tolerance

Active Publication Date: 2020-01-03
中国农业科学院研究生院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, relatively little research has been done on microbial biodegradation and soil remediation in e-waste contaminated areas

Method used

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  • Sphingobium yanoikuyae with function of degrading triphenyl phosphate
  • Sphingobium yanoikuyae with function of degrading triphenyl phosphate
  • Sphingobium yanoikuyae with function of degrading triphenyl phosphate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Isolation and Identification of Sphingobacterium sphingosinorrhea YC-XJ2

[0030] Soil samples were collected from the soil in the electronic waste pollution area and inoculated into 100mL inorganic salt ion medium containing 50mg / L TPhP, and cultured at 30°C and 180rpm. Every culture 7 days, get 10% of culture medium volume and inoculate in the fresh inorganic salt ion medium, and the concentration of TPhP is increased by 50mg / L each time, transfer continuously 5 times, in the inorganic salt medium, the TPhP concentration improves to 300mg / L.

[0031] Streak the acclimatized bacteria solution onto an inorganic salt medium plate containing 100mg / L TPhP, and culture it statically at 30°C for 4 days. Pick a single colony on the plate for streaking and culture, and repeat until a purified strain is isolated. The strain with good growth, stable passage and good degradation ability was preserved and named as YC-XJ2.

[0032] 1. Morphological characteristics of t...

Embodiment 2

[0039] Example 2 Degradation performance test of Sphingosinosinum serovar YC-XJ2

[0040] 1. Degradation of triphenyl phosphate by strain YC-XJ2

[0041] Inoculate strain YC-XJ2 into liquid LB medium for activation, and cultivate to logarithmic growth phase OD 600 =0.7, according to the inoculum size of 1% by volume, inoculate in the inorganic salt medium containing each 100mg / L mixture of triphenyl phosphate, and use the inorganic salt medium of 100mg / L triphenyl phosphate of the uninoculated strain as a control group , the control group and the treatment group each set up three replicates. The control group and the treatment group were cultured at 30° C., shaken at 180 rpm and protected from light, and samples were taken every 12 hours to detect the concentration of triphenyl phosphate.

[0042] Add an equal volume of acetonitrile to the triphenyl phosphate degradation sample, oscillate up and down to mix well, take 1 mL of TPhP degradation sample, and then filter it with ...

Embodiment 3

[0057] Application of embodiment 3 sphingosine bacteria YC-XJ2 in soil remediation

[0058] The soil used in this study was taken from the garden soil of the Chinese Academy of Agricultural Sciences. After the soil was removed with a 40-mesh sieve to remove large pieces of hard soil and sand, 10 g was weighed and placed in a 50 mL centrifuge tube, and the following two treatments were first performed: sterilization (121 ° C, 30 min) and non-sterilization.

[0059] Then the specific experimental procedure was carried out according to Table 1, YC-XJ2 bacterial solution was connected, and TPhP was added. For each sample, ensure that the sum of the volume of the added TPhP mother solution and the volume of the inorganic salt medium is 5 mL. Vortex to mix. The samples were placed in an outdoor natural environment for cultivation, during which they were shaken and mixed every 6 hours. After 2 days of cultivation, 10 g of soil samples were extracted to determine the concentration ...

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Abstract

The invention provides sphingobium yanoikuyae with a function of degrading triphenyl phosphate. The bacterium is named YC-XJ2, has a preservation number of CGMCC No.18049, and enables 100mg / L TPhP ininorganic salt to be degraded by 99.7 percent in 24 hours. The sphingobium yanoikuyae strain YC-XJ2 has strong adaptability on temperature, can keep the degradation rate on TPhP at 95 percent at 40 DEG C, can keep the degradation rate on TPhP at 88 percent or more when the pH value is 4-10, has a degradation at 74.8 percent or more when the salt concentration is 0-10 percent, and shows extremely strong salt resistance. The strain can be applied to biological repairing of a TPhP polluted environment and has relatively good economic value and application prospect.

Description

technical field [0001] The invention relates to the field of microorganisms and biodegradation, in particular to a strain of Sphingobium yanoikuyae (Sphingobium yanoikuyae) YC-XJ2 capable of degrading triphenyl phosphate. Background technique [0002] Triphenyl phosphate (TPhP) is an organic phosphate, which can be used as a flame retardant in foam, and can also be used as a plasticizer in nail polish and hydraulic oil. TPhP is an additive that does not form chemical bonds with polymer materials, which means it can be easily released into the environment. TPhP is neurotoxic and immunotoxic to organisms. [0003] In recent years, people have carried out some research on the biodegradation of TPhP, but with little success, only a few reports, Bacillus brevis (Brevibacillus brevis) can degrade 92.1% in 5 days at the initial concentration of TPP (3μmol / L) , which is already the fastest degradation rate reported for a single strain. Due to the difficulty in opening the phospha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C09K17/14C12R1/01C02F101/34
CPCB09C1/10C02F3/34C02F2101/34C09K17/14C12N1/205C12R2001/01
Inventor 闫艳春李先军
Owner 中国农业科学院研究生院
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