The present invention relates to an application of bacterial in quinoline degradation, wherein the bacterial is Ochrobactrum sp.. According to the present invention, the optimum degradation conditions are screened, wherein the optimum bacterial H1 growth temperature through utilizing quinoline is 30 DEG C, the initial growth pH value is 5.0, and a shaking table rotation speed is 150 r/min; the bacterial H1 meets a first order kinetic model when the initial quinoline concentration is within 250 mg/L, wherein a first order reaction rate is proportional to a quinoline concentration, the reaction that the bacterial grows through utilizing quinoline belongs to the first order reaction within the concentration range, the specific growth rate is increased along with concentration increase, and bacterial growth is not limited by the concentration within the concentration range; after the bacterial H1 is immobilized by using sodium alginate, a 400 mg/L quinoline degradation rate can be 87.5% within 24h; and the crude enzyme solution in the bacterial cell is extracted, wherein the crude enzyme solution can induce catechol 1,2-dioxygenase (C12O), nitrogen heterocyclic ring is firstly oxidized into quinolone during quinoline metabolism, and then the nitrogen heterocyclic ring is subjected to chain opening to form benzaldehyde, xylene, 2-pentanone, hydroxyphenyl propionic acid and other intermediate products.