Application of ochrobactrum in preparation of microbial fuel cell, and device and method for preparing microbial fuel cell

A pale bacillus and fuel cell technology, applied in the field of biochemical fuel cells, can solve the problems of hindering electricity production capacity, electricity producing bacteria and unclear electricity production mechanism, and achieve the effect of reducing requirements

Active Publication Date: 2014-03-12
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the electricity generation power of the mixed bacteria fuel cell is high, the further improvement of the electricity generation capacity is hindered by the unclear electricity generation bacteria and electricity generation mechanism.

Method used

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  • Application of ochrobactrum in preparation of microbial fuel cell, and device and method for preparing microbial fuel cell
  • Application of ochrobactrum in preparation of microbial fuel cell, and device and method for preparing microbial fuel cell
  • Application of ochrobactrum in preparation of microbial fuel cell, and device and method for preparing microbial fuel cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Take the Ochrobactrum sp.575 preserved in the laboratory, pick a single colony after streak culture and inoculate it into xylose medium, and culture it anaerobically at 37°C for 10 hours to OD 600 =0.8-1.0, then centrifuge the bacterial solution at 6000r / min, 4°C for 5 minutes to collect the bacterial cells, then re-add fresh xylose medium, mix well to make a bacterial suspension, and make the bacterial suspension OD 600 is 1.2.

[0035] Start the microbial fuel cell (Microbial Fuel Cell, MFC). The microbial fuel cell used in this example is two cylindrical pools (the effective volume of each pool is 100mL), with a layer of proton exchange membrane in between, and the two pools are connected by clips. together, and then sealed with a rubber stopper; the carbon brush is used as the anode and the cathode, and the titanium wire is used as the electronic current collector; the prepared bacterial suspension is added to the anode cavity of the microbial fuel cell, and then ni...

Embodiment 2

[0041] After the streak culture of three domesticated Pallidum pallidus obtained in Example 1, a single colony was inoculated into xylose medium, and anaerobic culture was carried out at 37°C for 10 hours to OD 600 =0.8-1.0, then centrifuge the bacterial solution at 6000r / min at 4°C for 5 minutes, collect the bacterial cells, add fresh xylose medium to the bacterial cells, mix well to make a bacterial suspension, and control the bacterial suspension OD 600 is 1.2.

[0042]Start the microbial fuel cell. The microbial fuel cell consists of two cylindrical pools (the effective volume of each pool is 100mL), with a layer of proton exchange membrane in between. The two pools are connected together by clips and sealed with rubber plugs; the carbon brush is used as the anode And cathode, use titanium wire wire as electronic current collector. The above-mentioned bacterial suspension is added into the anode chamber of the microbial fuel cell, and nitrogen gas is passed for 30 minute...

Embodiment 3

[0048] After the streak culture of three domesticated Pallidum pallidus obtained in Example 1, a single colony was inoculated into xylose medium, and anaerobic culture was carried out at 37°C for 10 hours to OD 600 =0.8-1.0, then take the bacterial solution and centrifuge at 6000r / min, 4°C for 5 minutes to collect the bacterial cells, then add the bacterial cells to fresh xylose medium, mix well to make a bacterial suspension, and control the bacterial suspension Liquid OD 600 is 1.2. At the same time, the cells were centrifuged in the same way and added to xylose-free medium to obtain a bacterial suspension as a control, and the fresh xylose medium that was not inoculated with cells was used as a blank control.

[0049] Add the above-mentioned bacterial suspension or xylose medium into the electrolytic cell respectively, and use an electrochemical workstation (CHI660E, Shanghai Chenhua) to perform a constant potential (0.2V) simulated discharge on the bacterial liquid for 12...

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Abstract

The invention discloses application of ochrobactrum in preparation of a microbial fuel cell, and a device and a method for preparing the microbial fuel cell. The ochrobactrum (Ochrobactrum sp.5753rd-generation) is preserved by the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO:M2013549, and the preservation date is November 6, 2013. The ochrobactrum can transfer electrons to an extracellular electron acceptor under an anaerobic condition, and can utilize xylose as a single carbon source to generate electric energy; the ochrobactrum is strong in electricity production activity, and has a good application prospect in recycling utilization of a biological energy source.

Description

technical field [0001] The invention belongs to the field of biochemical fuel cells, and in particular relates to the application of Pallidum bacillus in the preparation of microbial fuel cells, and also relates to the preparation method of the microbial fuel cell and the microbial fuel cell prepared by Pallidal bacillus. Background technique [0002] With the depletion of traditional fuels such as petroleum and coal, it is imminent to find new green and sustainable energy sources. Xylose is a renewable resource due to its widespread presence in agricultural waste such as corn stover. If xylose can be effectively used to generate electricity, it will not only help to deal with agricultural waste, but also help to solve the increasingly serious energy problem. [0003] Microbial Fuel Cell (MFC) oxidizes organic matter through the metabolism of microorganisms, and the electrons generated go through a series of transfers, reach the extracellular electrode, and then transfer to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20H01M8/16H01M4/86C12R1/01
CPCY02E60/527Y02E60/50Y02P70/50
Inventor 李鑫乔琰钟国祯李长明王行国
Owner SOUTHWEST UNIVERSITY
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