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Efficient plant growth promoting bacterium and application thereof in research and development of bio-compound fertilizer

A biofertilizer, a technology for promoting plant growth, applied in plant growth regulators, methods based on microorganisms, biochemical equipment and methods, etc. There are few types of growth-promoting strains to achieve the effect of promoting sustainable agricultural development, improving crop quality, and reducing soil degradation

Active Publication Date: 2015-12-02
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the plant growth-promoting strains have the following problems in the research and development and application of biological fertilizers: 1. The activity is unstable, and the activity drops seriously in actual production; the activity is high in a short time, and the activity decreases in a long time
2. Poor environmental adaptability, unable to effectively colonize, and the actual soil environment is relatively complex, making it impossible for the strain to colonize and show the same activity as under laboratory conditions; 3. The growth-promoting function is single, and some plant growth-promoting strains only have Single growth-promoting function of nitrogen fixation, phosphorus or potassium solution, does not have comprehensive plant growth-promoting properties
4. At present, there are few types of plant growth-promoting strains that can be used to develop bio-fertilizers, and the application range is relatively single and not targeted

Method used

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  • Efficient plant growth promoting bacterium and application thereof in research and development of bio-compound fertilizer
  • Efficient plant growth promoting bacterium and application thereof in research and development of bio-compound fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The screening method of embodiment 1 bacterial strain.

[0019] The specific method is as follows:

[0020] The culture medium formulation (w / v) used wherein:

[0021] Ashubei medium: glucose 1%, sodium chloride 0.02%, potassium dihydrogen phosphate 0.02%, calcium carbonate 0.5%, magnesium sulfate heptahydrate 0.02%, calcium sulfate 0.01%, agar powder 2%. The pH of the medium is 7.2,

[0022] Solid plate LB (Luria-Bertani) medium: sodium chloride 10%, tryptone 10%, yeast extract 5%, agar 2%, pH 7.5.

[0023] Liquid LB medium: 10% sodium chloride, 10% tryptone, 5% yeast extract, pH 7.5. Screening steps:

[0024] 1. Primary screening of solid plate culture medium

[0025] Samples were collected from lake water of Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, and serially diluted. The diluted solution was spread on Asbe solid medium, and cultured at 30°C for 72 hours. When a single colony appears on the Ashubei solid medium, transfer the...

Embodiment 2

[0030] This example illustrates the experimental application of the screened plant growth-promoting bacteria in pot experiments.

[0031] The specific method is as follows:

[0032] The soil in Anshan, Liaoning was selected as the soil for testing. The soil properties: pH6.8; organic matter 2.35%; total nitrogen 142mg / kg; total phosphorus 107mg / kg; total potassium 310mg / kg; available nitrogen 147mg / kg; Available potassium 376mg / kg. The soil to be tested was dried in a cool and dark place, and passed through a 1mm sieve. Mix every 200g of soil with 1.1g of tricalcium phosphate.

[0033] Cucumber Zhongnong No. 8 was used as the test plant. After the cucumber seeds were bred for 7 days, plants with the same size were selected as experimental plants. The nitrogen-fixing bacteria screened were carried out in a pot experiment, so as to screen out the efficient nitrogen-fixing bacteria that can promote the growth of plants. Every 200g of test soil is packed into flowerpots with ...

Embodiment 3

[0037] This example illustrates the growth-promoting properties of the screened plant growth-promoting bacteria Ochrobactrumsp.NW-3.

[0038] The specific method is as follows:

[0039] Determination of indole acetic acid: Inoculate the nitrogen-fixing bacteria into King's medium, and cultivate them on a shaker at 200 rpm at 30°C for 24 hours. Take 3ml of the bacterial solution and centrifuge at 12000rpm for 15min to remove the precipitate. Take 1ml of supernatant and add 2ml of Salkowski's reaction solution. After mixing and reacting in the dark for 30 minutes, observe its discoloration. If the color turns pink, it is considered to produce 3-indoleacetic acid. The darker the color, the stronger the ability to secrete 3-indoleacetic acid; 3-Indoleacetic acid.

[0040] Detection of siderophore: Culture nitrogen-fixing bacteria in beef extract peptone liquid medium for 24 hours on a shaking table, absorb 2 μL of the bacterial solution and spot it on a CAS plate, culture at 30...

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Abstract

The invention provides an efficient plant growth promoting bacterium Ochrobactrum sp.NW-3 which is collected under number of CGMCC No.10858. The efficient plant growth promoting bacterium is used for producing hormone IAA, ACC deaminase and ironophore and is further used for producing products and bio-fertilizer for promoting plant growth. By the microbial fertilizer, soil pollution, water pollution and threat to human health caused by chemical fertilizer can be reduced greatly, soil can be improved, soil fertility can be enhanced, agricultural non-point source pollution can be reduced, green agriculture can be developed, and sustainable development of agriculture can be promoted, so that the bio-compound fertilizer has wide application prospect and huge market potential.

Description

technical field [0001] The invention belongs to the technical field of biological fertilizer research and development and application, and specifically relates to the research and development of a high-efficiency plant growth-promoting bacterial strain and a set of biological compound fertilizer with the bacterial strain as the core and its application in promoting growth of cucumbers in Northeast China. Background technique [0002] The use of chemical fertilizers has played a huge role in increasing agricultural production and income in our country, but with the extensive use of chemical fertilizers, it has also brought some problems. For example, the utilization rate of chemical fertilizers continues to decline. In the past, 1 catty of chemical fertilizers could increase grain production by 20 catties, but now it can only increase grain production by 10 catties, and some can only increase yields by 7-8 catties, or even less. In the last 10 years, the total consumption of ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/88A01P21/00C05G3/00C12R1/01
Inventor 徐松黄志勇王敬敬柳影
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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